FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a39.3510000003f4c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a39.3510000003f4c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199626
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC45992.303808121186619No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA33141.6601043952190597No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT31741.589973249977458No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG27051.3550339134180918No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT23811.192730405858956TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA22211.1125805255828398No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT21431.0735074589482332No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG19110.9572901325478645No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT16070.8050053600232434No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14590.7308667207678359No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14360.7193451754781441No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT13890.695801148147035No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13820.6922945908849548No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT8960.4488393295462515No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA8940.4478374560428No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA8540.4277999859737709No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT6960.3486519792011061No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA6550.32811357238035127No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA5990.3000611142837105No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5110.25597868013184655No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA4040.20237844769719374No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA3700.18534659813851903No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG3420.17132036909019865No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA2910.1457725947521866No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG2340.11721919990382015No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT2010.10068828709687114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA20900.0294.294251
GGGTTAT21300.0288.76762
GTTATTA22550.0273.41464
GGTTATT22700.0270.958163
GGGGGGA16650.0269.309331
TTATTAG23150.0266.32835
GGGAGCG12400.0258.1254
TTAGTAG23950.0258.0488
GGAGCGA12450.0257.088385
ATTAGTA24250.0254.247427
TATTAGT24350.0253.20336
GGGGAGC17800.0251.910133
TAGTAGA25300.0243.695659
GAGCGAA14450.0221.50526
AGCGAAA14650.0218.481237
GGGAGCA5900.0217.54
CGAAAGC14750.0216.09
GGGGGAG21200.0212.205182
GATACGA150.0028479402196.66667295
GCGAAAG16500.0193.09098
Produced by FastQC (version 0.11.2)