FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a3.3510000003f416.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a3.3510000003f416.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209969
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATCAATGCACTCAAATGCA97194.628778533974063No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC58872.803747219827689No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT39361.8745624354071317No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG36041.7164438559977901No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG35421.6869156875538769No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC23831.1349294419652425No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA22121.0534888483538045No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT20790.9901461644337974TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT19860.9458539117679277No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA19580.9325186098900314No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG18100.8620320142497226No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT16770.7986893303297153No Hit
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT16610.7910691578280603No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT14400.6858155251489506No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG13820.6581923998304512No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA10390.494834951826222No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT10390.494834951826222No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG10100.4810233891669723No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA9490.4519714815044125No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8630.41101305430801693No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8130.38720001524034503No Hit
GGGGGGAGCAAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT7660.3648157585167334No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT7280.34671784882530277No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT5210.24813186708514115No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA4250.20241083207521113No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG3840.18288414003972014No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA3500.16669127347370327No Hit
CGGGTTATTAGTAGAAACAAGGCCCCAACTTCTTAGAGGGACAAGTGGCG3380.160976144097462No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3120.14859336378227261No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA3020.14383075596873823No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT3000.14287823440603137No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA2410.11477884830617853No Hit
GGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTG2350.1119212836180579No Hit
GGGGGGAGCAAAAGCTATACTAAGAAAAGCAACCAGAAGATTGGTTCAAC2330.11096876205535103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT35950.0283.511842
GGGGGGA25400.0282.80511
CGGGTTA36950.0275.43981
GGTTATT37150.0273.956943
GTTATTA37100.0273.5314
TTATTAG37750.0269.211945
TATTAGT38600.0263.28376
TTAGTAG38700.0262.98458
GGGAGCG20150.0260.595554
GGAGCGA20100.0260.509955
GGGGAGC27600.0260.26273
AAACGTT400.0258.125295
ATTAGTA39700.0256.731727
TAGTAGA40500.0250.932119
CGAAAGC21450.0244.114219
GGGGGAG31050.0231.819662
GAGCGAA22750.0230.164846
GCGAAAG22850.0229.803058
AGCGAAA23000.0227.663067
GGGAGCA8800.0219.573884
Produced by FastQC (version 0.11.2)