FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a29.3510000003f64b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a29.3510000003f64b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237547
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA193738.15543871318097No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG132195.564793493498129No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG99924.206325485061903No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA79183.3332351071577415No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT78953.323552812706538No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA76093.2031555860524445No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC63232.661789035432988No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG41971.7668082526826272No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT41221.7352355533852246TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT40491.7045047927357535No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG38111.604314093631997No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA32121.352153468576745No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA28911.2170223155838635No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA19620.8259418136200415No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT18710.7876336051391936No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT17040.717331728036978No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT15810.6655525011892384No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG15250.641978219047178No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA12940.5447343052111793No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG11420.4807469679684441No Hit
GGGGGGAGCGAAAGCAGGTCAATTAAATTCAGTATGGAAAGAATAAAAGA9620.40497248965467886No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT8450.3557190787507314No Hit
GGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAA6190.26058001153455945No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA6150.258896134238698No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5840.24584608519577178No Hit
GGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGC4690.1974346129397551No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAGCTGAAATGAG4440.186910379840621No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA4110.17301839214976406No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT3970.16712482161424896No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT3790.15954737378287243No Hit
GGGGGGAGCAAAAGCAGGTCAATTAAATTCAGTATGGAAAGAATAAAAGA3570.15028604865563447No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC3190.1342892143449507No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT3090.13007952110529708No Hit
GGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTA2960.12460691989374735No Hit
CGGGTTATTAGTAGAAACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT2770.11660850273840545No Hit
GGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAA2560.10776814693513284No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT2510.10566330031530602No Hit
GGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCC2510.10566330031530602No Hit
GGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAA2500.10524233099134066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA63750.0291.760771
GGGGGGA51250.0290.970731
GGGTTAT64350.0289.04042
TTATTAG64800.0286.805545
TTAGTAG65250.0284.827588
GTTATTA65600.0283.532784
GGTTATT65700.0283.101233
TATTAGT66050.0281.377726
ATTAGTA66450.0279.6847
TAGTAGA67150.0276.76849
GGGGGAG56200.0265.34252
GGGGAGC56100.0264.763823
GGGAGCG40300.0258.76554
GGAGCGA40650.0256.900365
GAGCGAA41850.0249.534046
AGCGAAA42100.0248.052267
GGGAGCA17950.0246.51814
CGAAAGC42700.0244.566749
GCGAAAG43350.0240.899648
CAAAAGC19400.0222.770639
Produced by FastQC (version 0.11.2)