FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a252.3510000003f671.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a252.3510000003f671.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210317
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC94034.470870162659224No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA49142.3364730383183483No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT47862.275612527755721TruSeq Adapter, Index 7 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCCTTAATTGTCGTACTCT47062.2375747086540794No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG38501.830570044266512No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT19710.9371567681167No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG19010.9038736764027635No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT15560.7398355815269332No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA15490.7365072723555395No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG15460.735080854139228No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT13190.6271485424383193No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA12180.5791257958224965No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA10630.5054275213130655No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA9390.4464689017055207No Hit
GGGGGGAGCGAAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATC8500.4041518279549442No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7940.37752535458379494No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7130.33901206274338264No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6260.29764593447034715No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5800.2757741884869031No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG4800.22822691460985084No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT3440.16356262213705977No Hit
GGGGGGAGCAAAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATC3140.1492984399739441No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA3110.14787202175763253No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA2940.13978898519853364No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2830.1345587850720579No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA2450.11649082099877803No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG2390.11363798456615488No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA2210.1050794752682855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA27100.0291.190031
GGGGGGA19150.0287.297641
GGGTTAT27700.0284.882662
GTTATTA28850.0273.52694
GGTTATT29100.0271.176973
TTATTAG29450.0267.954165
ATTAGTA30100.0262.167767
TTAGTAG30100.0262.167768
TATTAGT30300.0260.43736
GGGGAGC21050.0259.964363
GGAGCGA14600.0259.64045
GGGAGCG14600.0259.64044
TAGTAGA31350.0251.714529
CGAAAGC15750.0240.682549
GAGCGAA16150.0234.721366
GGGAGCA7200.0233.541664
AGCGAAA16350.0231.850177
GGGGGAG24050.0228.149692
GCGAAAG17200.0220.392448
AGACCCC150.0028480485196.66666295
Produced by FastQC (version 0.11.2)