FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a20.3510000003f5a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a20.3510000003f5a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244780
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC70362.8744178445951465No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG43801.789361875970259No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCCT43451.7750633221668435No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA40261.6447422175014297No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG39951.6320777841326906No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA34871.4245444889288341No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGAACAAGCTAAATT27941.141433123621211No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT26121.0670806438434512No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT25501.0417517771059728No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA22570.9220524552659531No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT21700.8865103358117493TruSeq Adapter, Index 2 (97% over 37bp)
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG20700.8456573249448485No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTT17950.7333115450608709No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA14100.5760274532233026No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12750.5208758885529864No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11980.4894190701854727No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA11180.45673666149195197No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT9370.38279271182286134No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA6790.27739194378625703No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTT5870.23980717378870825No Hit
GGGGGGAGCGAAAGCAGGAGTGAAGATGAATCCAAATCAAAAGATAATAA4350.17771059727101887No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT3110.12705286379606176No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATGC108.460964E-4295.0295
TACCGCG108.460964E-4295.0295
CGGGTTA28800.0293.463561
GGGTTAT29250.0289.4532
GGGGGGA24850.0284.31591
GTTATTA30050.0282.23794
GGTTATT30450.0278.53043
TTATTAG30750.0275.8135
GGGGAGC26950.0263.803343
GGGGGAG27050.0262.82812
GGGAGCA8300.0261.234954
TATTAGT32550.0259.654366
GGGAGCG19100.0258.704164
TTAGTAG32750.0258.06878
GGAGCGA19150.0258.028725
TAGTAGA33050.0256.172459
ATTAGTA34200.0247.12727
CGAAAGC20400.0242.218129
GCGAAAG21150.0233.628858
AGCGAAA23150.0212.807797
Produced by FastQC (version 0.11.2)