FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a2.3510000003f409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a2.3510000003f409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201747
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA145017.187715306795145No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC84154.171065740754509No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA77743.8533410657903215No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG73323.6342547844577613No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG50692.512552850847844No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT37041.835962864379644No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA35501.7596296351370777No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG29851.4795759044744161No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT29551.4647057948817082TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA24641.2213316678810588No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG19080.9457389700962096No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT15350.7608539408268772No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT13980.6929471070201787No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA13930.6904687554213941No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG10820.536315285976991No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG8040.3985189370845663No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT8040.3985189370845663No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT6140.30434157633075093No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5800.2874887854590155No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5800.2874887854590155No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT5320.2636966101106832No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT4590.2275126767684278No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT4080.2022334904608247No Hit
GGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGC3930.19479843566447083No Hit
GGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAA3850.19083307310641548No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA3310.1640668758395416No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATACAATGCTTCAACCCGAT2970.14721408496780622No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2300.11400417354409235No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA2240.11103015162555081No Hit
GGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCC2160.10706478906749542No Hit
GGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAA2090.10359509682919697No Hit
GGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAA2030.10062107491065543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGC157.2315343E-6294.99997295
CGGGTTA39050.0292.355961
GGGGGGA31350.0284.649141
GGGTTAT40250.0284.006232
GGTTATT41050.0278.112063
GTTATTA41450.0275.428224
TTATTAG41800.0272.769135
TTAGTAG42200.0269.83418
TATTAGT42850.0266.08526
ATTAGTA43050.0264.849037
GGGGAGC34850.0256.061683
GGGAGCG26400.0246.392044
AAACGTT301.5770638E-9245.83331295
GGAGCGA26550.0245.05
CGAAAGC27050.0240.471349
GCGAAAG27650.0235.786628
GGGGGAG38300.0233.766312
GAGCGAA28000.0232.83936
AGCGAAA28100.0232.010687
TAGTAGA49350.0230.739619
Produced by FastQC (version 0.11.2)