FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a16.3510000003f5b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a16.3510000003f5b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229077
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG200908.769976907328104No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA115145.026257546589139No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC63532.773303299763835No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA59262.586903093719579No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA40491.7675279491175457No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT39741.734787866088695No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT38151.665378890067532No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG32691.4270310856175No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA23361.0197444527385988No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA21230.9267626169366633No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT20840.9097377737616609TruSeq Adapter, Index 7 (97% over 38bp)
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT20060.8756880874116564No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA19770.8630285886405008No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT17720.7735390283616427No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG13750.600234855528927No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT10040.43828057814621285No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG10030.4378440437058282No Hit
GGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAA9970.4352248370635201No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA8320.3631966544000489No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7040.3073202460308106No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6680.29160500617696233No Hit
GGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGA5140.2243787023577225No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT4800.2095365313846436No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG4510.19687703261348802No Hit
GGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAA3820.16675615622694553No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAGCTGAAATGAG3200.13969102092309574No Hit
GGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAAC3120.13619874540001833No Hit
GGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGAT2730.11917390222501605No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT2490.10869707565578385No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG108.4606104E-4295.08
AAACGTT206.174196E-8295.0295
CGGGTTA47200.0292.51
GGGGGGA32950.0290.52351
GGGTTAT48550.0284.366642
GGTTATT48900.0282.029663
GTTATTA49100.0280.880864
TTATTAG49450.0278.892825
TTAGTAG50000.0276.128
TATTAGT50250.0274.746256
ATTAGTA51000.0270.70597
GGGGAGC36000.0266.319433
GGGAGCG26350.0264.772284
GGAGCGA26600.0262.283845
TAGTAGA53050.0260.245069
CGAAAGC27050.0256.284679
GCGAAAG27300.0254.478038
GAGCGAA28600.0243.426596
GGGGGAG39450.0243.029172
AGCGAAA28600.0242.910847
Produced by FastQC (version 0.11.2)