FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a132.3510000003f58f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a132.3510000003f58f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225490
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC88673.932325158543616No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT54162.4018803494611736No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG48452.148654042307863No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA42901.9025233934986032No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG34991.551731784114595No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT31061.3774446760388488TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT28011.2421836888553817No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA26911.1934010377400328No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT26821.1894097299215043No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15290.6780788505033483No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA15130.6709831921592975No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG12570.5574526586544858No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA12230.542374384673378No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT11110.4927047762650228No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7810.34635682291897646No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT6740.29890460774313715No Hit
GGGGGGAGCGAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG6300.27939154729699767No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6010.2665306665484057No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4160.18448711694531908No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT3850.1707392789037208No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA3240.14368708146702736No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT3220.14280012417402102No Hit
GGGGGGAGCAAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG2770.12284358508137833No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA2490.11042618297928955No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG2340.103774003281742No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTGC108.460522E-4295.0295
CGGGTTA30500.0292.5821
GGGGGGA17650.0290.821531
GGGTTAT30900.0289.271852
GGTTATT31300.0285.575073
GTTATTA31400.0285.135354
TTAGTAG32650.0274.219028
TTATTAG32950.0272.169985
TATTAGT33150.0270.527926
ATTAGTA33650.0266.069857
GGGGAGC19300.0265.958563
TAGTAGA34000.0263.76479
GGAGCGA15050.0262.65785
GGGAGCG15050.0262.65784
CGAAAGC15300.0259.330089
GGGAGCA4900.0240.816334
GCGAAAG16650.0238.303318
GAGCGAA17300.0229.349726
AGCGAAA17350.0228.688757
GGTCAGC202.279695E-5221.25295
Produced by FastQC (version 0.11.2)