FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a12.3510000003f5c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a12.3510000003f5c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223962
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG142716.372063117850349No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA84003.7506362686527175No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC62992.812530697171842No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA49362.2039453121511685No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT44872.003464873505327No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG34061.5207937060751378No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA32591.4551575713737153No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT28421.2689652708941697No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA26481.1823434332609997No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT25921.1573391914699815TruSeq Adapter, Index 13 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT21090.9416776060224502No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA20350.9086362865128906No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA18150.8104053366196051No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG15600.6965467356069334No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14690.6559148426965289No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12310.5496468150847019No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG10760.48043864584170526No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT9330.4165885284110697No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA8460.3777426527714523No Hit
GGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAA7380.3295201864602031No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6220.2777256856073798No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6110.27281413811271554No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5630.25138193086327143No Hit
GGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGA3250.14511390325144444No Hit
GGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAA2810.12546771327278736No Hit
GGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAAC2590.11564461828345879No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT2530.1129655923772783No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT2490.11117957510649128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAGC108.460486E-4295.0295
GGTATGC206.173832E-8295.0295
GGTCTGG157.2325965E-6295.0295
CGGGTTA42600.0292.230041
GGGGGGA26700.0287.265931
GGGTTAT43500.0285.844852
GTTATTA44550.0279.438844
GGTTATT44700.0278.171143
TTATTAG44900.0277.589085
TTAGTAG45400.0273.882148
TATTAGT46200.0269.778146
ATTAGTA46350.0268.26867
TAGTAGA48100.0258.814979
GGGGAGC29850.0255.963153
GGGAGCG20850.0253.968834
GGAGCGA21050.0251.555825
CGAAAGC21300.0247.91089
GCGAAAG21500.0245.604668
GAGCGAA22900.0231.233636
AGCGAAA22850.0231.09417
Produced by FastQC (version 0.11.2)