FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a1116.3510000003f68e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a1116.3510000003f68e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187430
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA89384.768713653097157No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC71233.8003521314624122No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG55192.9445659712959507No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT44532.375820306247666No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA36731.9596649415781893No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC33771.8017393160113109No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG33421.783065677853065No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG32631.7409166088673105No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT25981.3861174838606414TruSeq Adapter, Index 13 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA23691.2639385370538334No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA21721.1588326308488501No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT19101.0190471109214105No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG16120.8600544203169183No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG12500.6669156485087766No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA11710.624766579523022No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG9670.5159259456863896No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC9380.500453502640986No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6810.3633356453075815No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA5730.3057141332764232No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC5680.3030464706823881No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA5640.30091234060716004No Hit
CGGGTTATTAGTAGAAACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT5380.2870404951181774No Hit
CGGGTTATTAGTAGAAACAAGGGCAGGGACTTAATCAACGCAAGCTTATG4110.21928186522968576No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC3920.20914474737235234No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA3680.19633996692098385No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA3510.18726991410126448No Hit
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT2900.1547244304540362No Hit
CGGGTTATTAGTAGAAACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC2700.14405378007789574No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT2660.14191965000266768No Hit
GGGGGGAGCGAAAGCAGGTGCATTGATTGAAGATCCAGATGAAGGCACAT2580.1376513898522115No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT2560.13658432481459745No Hit
GGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGT2430.1296484020701062No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA2340.124846609400843No Hit
GGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAAC2110.1125753614682815No Hit
GGGGGGAGCGAAAGCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC2090.11150829643066745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA21550.0291.577731
CGGGTTA42000.0286.571441
GGGTTAT42650.0283.24152
GGTTATT43600.0276.731663
ATTAGTA43750.0275.108587
GTTATTA44450.0271.107974
TATTAGT44850.0268.690066
TTATTAG44950.0267.76425
TAGTAGA45550.0263.91339
TTAGTAG45850.0262.18658
GGGGGAG24250.0259.11342
CGAAAGC18300.0246.639349
GCGAAAG18350.0245.96738
GGACTCG301.5770638E-9245.83334295
GAGCGAA18250.0245.698646
GGAGCGA18300.0245.027335
GGGGAGC26500.0236.556613
GGGAGCG19450.0230.539864
AGCGAAA20450.0219.987767
TCCCTAC500.0206.59
Produced by FastQC (version 0.11.2)