FastQCFastQC Report
Thu 25 Feb 2016
000000000-AKKFY_l01n03.3330000004aed1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKKFY_l01n03.3330000004aed1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15357180
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTTGGGCAT493088032.10797815744817No Hit
GGATACCGTGTTACGACTTCTTCATCCTCTAGATGATAAGGCTCACGGTA178894211.648896477087591No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC14034799.138910919843358No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAGGCG7256684.725268571443455No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAG5531373.6018136142182353No Hit
GGCTACCTTGTTACGACTTAGTCCCCCTCACCAGACTCACCTTCGGCCGG1227920.799573880100383No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCTTACCGTGGTCGGC1078820.7024857428251801No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGGCCCTACCGTAGACGGC1006140.6551593456611174No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATTGGGTTCGTATAA965980.6290087112347449No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGATTCACTGTT589910.38412651281029464No Hit
GGCTACCTTGTTACGACTTAGTCCCCCTCACCAGACTCACCTTCGACCGG466720.30390996263636944No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAACTAATCAAGTTTGGGCAT414660.27001050974202295No Hit
AGTTACCTTGTTACGACTTTTACTTCCTCTAAACGGCCAAGTTTGACCAG399830.26035378891176636No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATCGACCCCACCGTGATCGGC380420.24771474971316348No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCAGCCCTACCGTAGACGGC341040.22207202103511192No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACTCCGTGGTAACC294560.19180604772490784No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTTGGCCAT222410.1448247660052171No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGACCAAGTTTAACCAG184840.12036063912775653No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATCAACCTCACCGTGATCGGC184380.12006110496849032No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGACCCTGCCTTGGGCGCC180480.1175215762268854No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTCCAAGCCACTGCGATTAAGGCG178310.11610855638860781No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTACATAATCAAGTTTGGGCAT156990.10222579926783433No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGGCCCTACCGTAGACTGC154450.10057184977971215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCG2406250.093.784292
ACCTTGT11636650.093.779125
TGTTACG14075350.093.77879
CTTGTTA11641650.093.7779167
CCTTGTT11656100.093.777146
ACCGTGT2442950.093.774845
CCGTGTT2447800.093.7595446
CGTGTTA2446000.093.740357
TTGTTAC11639650.093.729168
AAACCTT8663500.093.7025453
AACCTTG8691900.093.644584
GAAACCT8676850.093.637832
GGATACC2436850.093.557311
GGAAACC8677800.093.5481341
GTGTTAC2453250.093.4843448
GGCTACC842800.093.398951
ATACCGT2414650.093.360933
GTTACCT2017850.093.145612
GAGACCT13900.093.1192552
TTACCTT2020950.092.94243
Produced by FastQC (version 0.11.2)