FastQCFastQC Report
Thu 25 Feb 2016
000000000-AKKFY_l01n02.3320000004aed4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKKFY_l01n02.3320000004aed4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15357180
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCGTATTGG13675318.904831485988964No Hit
TGAGAACCTCCA11766267.661732166973363No Hit
CTAACCTCCGCT7875535.12823968983889No Hit
ATTTCGACATGC7813445.087809089950108No Hit
TATCGTTGACCA7427224.836317605185327No Hit
GAGTGAGTACAA7100474.623550677923942No Hit
TTACTGTGCGAT5840983.803419638240875No Hit
GCGGATGTGACT5715563.7217509985557244No Hit
TAATGACCACGC5631763.6671836886720084No Hit
TGCCCATTTAAG5240833.412625234580828No Hit
TTCAGACTACCA4638653.020508973652715No Hit
AACGGATAAACG4366512.8433019603859564No Hit
AATCAGTCTCGT3801992.4757084308447252No Hit
AGTTCAGACGCT3745992.4392434027601424No Hit
AGCAGTCGCGAT3483772.2684959087540815No Hit
TCCGACACAATT3349342.1809603065146077No Hit
CAGGGTGATACC3073942.0016305076843537No Hit
CTTATCAAGGCG2988171.9457804102055196No Hit
TACACGATCTAC2808361.8286951119932175No Hit
TGCCTACAAGCA2799471.82290628878479No Hit
AATCCGTACAGC2765721.8009295977516704No Hit
CCAGTGTATGCA2420071.5758557235117383No Hit
CCTCGTTCGACT2290341.4913805789865067No Hit
ACCGTGCATAGG2214291.441859768525211No Hit
ATCGAATGCGGC2133571.3892980351861475No Hit
GGAGACAAGGGA1939531.2629467128730665No Hit
TGAGTCACTGGT1890791.2312091152151632No Hit
GTATGCGCTGTA1825251.188532009131885No Hit
AGCTGTTGTTTG1377660.8970787605536955No Hit
TGAAAACCTCCA192700.1254787662839141No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGT1303.6470738E-96.00000052
CGGCGCA650.00126196546.00000052
TCGTTGC5650.06.00000053
CCGGGTG1303.6470738E-96.00000051
AGCCATT650.00126196546.00000052
TGTATTG2600.06.00000055
GGTCAGA650.00126196546.00000052
AGGCGTC7300.06.00000052
CACTGGA1303.6470738E-96.00000056
TCAATTG650.00126196546.00000055
ACGATCC650.00126196546.00000054
TCATGGC650.00126196546.00000055
ACTACCG650.00126196546.00000056
GACGGAT1303.6470738E-96.00000051
CAAGAGA650.00126196546.00000056
CAATGGA2750.06.00000056
GTTGGCC650.00126196546.00000055
TGTCGCG650.00126196546.00000054
TATCATG909.152358E-66.03
CAGGTTG1101.8153332E-76.01
Produced by FastQC (version 0.11.2)