FastQCFastQC Report
Thu 25 Feb 2016
000000000-AKKFY_l01n01.3310000004aed7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKKFY_l01n01.3310000004aed7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15357180
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGCGGGCGCGG607770239.575638235665664No Hit
GCTTCAACCGATTGGATGATCCGGCAAACTGTACGGACATTTGGAATCAC225737614.699157006690031No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG180447211.75002181390073No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACCGCTGTTTCGAC9447906.152106050720249No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAAGA4984433.2456674988507004No Hit
ACACCATGGGAGTGGGTTATACCAGAAGGCGGTAGATTAACCTCGCAAGA1369740.8919215637245901No Hit
GCTCCTACCGATTTCGAGTGATTCGGTGAGTCTTTTGGACCTGTCACAAG1104520.7192205860711407No Hit
AAGCCACCCAAGCTGGAAGCACCCGAAGTCCGGCGCCAATTGTCCGGCCG977930.6367900877635086No Hit
GCTCCTACCGATTGAATGATCAGATGAAATGTTCGGATGGAATAGTTGCA723750.47127792993244855No Hit
AAGCCACCCAAGCTGGAAGCACCCGAAGCCCGGCGCCAATTGTCCGGTCG539900.35156194040833016No Hit
ACACCATGGGAGTTGATTTTACCTTAAGTCGGTGAGCTAACCAGCAATGG523370.3407982455112202No Hit
GCTACTACCGATGAATGGTTAAGTGAGGCCTTCGGATTGGCCGACGCGTG508730.33126524531196483No Hit
ACACCACGAAAGTTGGTTTTACCCGACACCGGTGGGCTAACCCGCAAGGG436710.2843686145503276No Hit
ACACCATGGGAGTGGGTTGCTCCAGAAGTAGCTAGGCTAACCTCGTGAGG431420.28092397171876604No Hit
ACACCACGAAAGTTGGTTTTACCCGACACCGGTGAGCTAACCCGCAAGGG334520.21782644990812114No Hit
ACACCATGGGAGTTGATTTTACCTTAAGCCGGTGAGCTAACCAGCAATGG244600.15927403338373322No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGCGGGCGCAG233880.1522935851503987No Hit
ACACCACGAAAGTTGGTTTTACCCGAAGCCGGTGAGCTAACCATTTATGG233220.15186381874797325No Hit
AAGCCACCCAAGCTGGAAGCACCCGAAGTCCGGCGTCAATTGTCCGGCCG227890.14839312946777988No Hit
AAGCCACCCAAGCTGGAAGCACCCGAAGTCCGGCGCCAATAGTCCGGCCG221150.14400430287331398No Hit
GTTACTACCGATTGAATGGCTTAGTGAAGCCTCCGGATGGGATCAGACAA219420.14287779396998668No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGCGGGCGCCG199830.1301215457525405No Hit
GCACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG193270.1258499281769179No Hit
GCCTCAATCGACTGGATGTTTCGGTGAATTATTTGGACCGCGGTCAACGC185670.12090110293686732No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGG171080.11140066079840177No Hit
ACACCACGAAAGTTGGTTTTACCCGAAGCCGGTGAGCTAACCCGCAAGGG168780.10990299000207068No Hit
GCTACTACCGATTGAATGATTTAGTGAGGCCTTCGGACCGGCGGGCGCGG165630.1078518321723129No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGGCCGGCGGGCGCGG161780.10534486149149778No Hit
ACACCACGAAAGTTGGTTTTGCCAGACGTCGCCGGACTAACCCGCAAGGG155680.10137277807514139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG254.623398E-795.07
GCACCTA353.3105607E-1095.01
CGATCGA9400.095.09
ATCTGTG201.7445203E-594.999994
TGGTACC2200.094.999993
CCATGGG2335250.094.987794
AACCGAT2428300.094.9843446
CTAGTAC591200.094.9839252
CATGGGA2327100.094.981635
ACCATGG2293000.094.979293
GCTAGTA585450.094.975661
CTTCAAC2430050.094.974582
CAACCGA2430000.094.972635
CGATTGA7511150.094.9702769
CGGGACT1028200.094.944567
GGGAGTG2199200.094.922248
GCCACCC234550.094.898743
TCCTACC244200.094.8832863
GGGACTG1030550.094.880168
TGGGAGT2331400.094.8370067
Produced by FastQC (version 0.11.2)