FastQCFastQC Report
Mon 30 Nov 2015
000000000-AKKC9_l01n03.3330000003ede3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKKC9_l01n03.3330000003ede3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6499951
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA4147236.380401944568505No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA3655825.624380860717258No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG3060434.7083893401657955No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2610174.015676425868441No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAA2492083.833998133216696No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT2419433.7222280598730664No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA2257763.473503107946506No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA1269551.9531685700399894No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1170211.8003366486916594No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGTTATCCCAGGA919821.4151183601230224No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG910281.4004413264038453No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG814121.2525017496285742No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG748721.1518856065222647No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGTTGGTCCAGGT732321.126654647088878No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG691961.0645618713125684No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGAA679881.0459771158274886No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA499770.7688827192697298No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGGAACCGGCCAGCA417790.642758691565521No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA412130.6340509336147303No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG407560.6270201113823781No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG390650.6010045306495387No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA317930.48912676418637624No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTACCCAGAG303780.4673573693093994No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG296270.4558034360566718No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT290200.4464649041200464No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA284200.43723406530295383No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG264590.4070646071024228No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAATGTTGGTCCAGGT262500.4038491982478022No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG236410.3637104341248111No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG225750.3473103104931099No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT215250.3311563425631978No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGATGGTCCAGTT210320.32357167000182No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGGAAAGGCCCAGAG206720.31803316671156445No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG200790.30891002101400455No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTAATGGCCAGAA169690.26106350647874116No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA155040.2385248750336733No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA152000.23384791669967975No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGAATGAGCCCAGTA144920.22295552689551046No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG140480.21612470617086188No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA136690.21029389298473172No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA129480.19920150167285874No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG124890.1921399099777829No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCCCTTT124260.1911706719019882No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC121270.18657063722480371No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATATCTTGGTA117460.18070905457594988No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG114420.1760320962419563No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCACTGTTAGACCAGAA103700.15953966422208415No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG102440.1576011880704947No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTCAGACCAGAA101350.1559242523520562No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC100350.1543857792158741No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGAG96700.1487703522688094No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG89390.13752411364331824No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA87640.13483178565499956No Hit
CCTGTTTGCTACCCACGCTTTCGAATCTCAGTGTCAATATTATGCCAGGA86730.13343177510107385No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA85920.13218561186076633No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG82140.12637018340599798No Hit
CCTTCTCGCTCCCCAAGGCTTCGTCCCTCAACGTCAGTTTTTAGTAGAAG80030.12312400508865373No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG79360.12209322808741174No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAATAGGCCAGCA79080.12166245560928075No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGTGTCAGTATTAGGCCAGAT76320.11741626975341814No Hit
CCTGTTCGCTACCCATGCTCTCGCTCCTCAGCGTCAGTGGCTGCCCAGAG74020.1138777815401993No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTTAGACCAGAA73600.11323162282300281No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG70290.10813927674224007No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTGTTGCCCAGAG70080.10781619738364182No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG69040.10621618532201244No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG66200.10184692161525526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC2465350.0244.701863
CTGTTCG1361750.0244.109422
TTGGGTC284000.0243.274667
CCTGTTC1356000.0243.256471
TTTGCTC1477900.0243.251075
GTTTGGG283550.0242.407875
TGTTTGA486500.0241.902883
TTTGATC434350.0241.813065
TTTGGGT285600.0241.654426
TTGCTCC1512850.0241.501976
CTGTTTG2988250.0240.953922
GTTTGCT2509200.0240.674534
GATCCCC457300.0238.704938
TGTTCGC1364750.0238.582173
GGGTCTT289700.0238.530389
TGATCCC449900.0237.621157
TTGATCC450800.0237.445666
TTTGCTA1060300.0236.196375
TGCTACC1078950.0236.007927
CTCCCCA2239850.0235.970499
Produced by FastQC (version 0.11.2)