FastQCFastQC Report
Mon 30 Nov 2015
000000000-AKKC9_l01n02.3320000003ede6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKKC9_l01n02.3320000003ede6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6499951
Sequences flagged as poor quality0
Sequence length12
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATCGCAGATC3400325.23130097442273No Hit
GTCTAATTCCGA1748092.6893895046285734No Hit
AGATGTTCTGCT1623892.498311141114756No Hit
GTTGGTCAATCT1571472.4176643793160904No Hit
CTAACCTCCGCT1316942.0260768119636596No Hit
GACTCGAATCGT1268571.9516608663665311No Hit
GAATCTTCGAGC1220941.8783833908901773No Hit
TAAACCGCGTGT1185691.8241522128397585No Hit
ATGTGCACGACT1113491.7130744524074104No Hit
GAACCAAAGGAT1021621.5717349253863606No Hit
ATGTCGAGAGAA1000421.5391192948993No Hit
GTATGCGCTGTA978071.50473442030563No Hit
TATGCACCAGTG965931.4860573564323794No Hit
ACTCACGGTATG952361.4651802759743882No Hit
CTAGATTTGCCA930711.4318723325760456No Hit
CTCTACCTCTAC870881.3398254848382702No Hit
ACACCTGGTGAT847411.3037175203320763No Hit
CTTGTGTCGATA844631.2994405650134901No Hit
CGCAGCGGTATA830141.2771480892702114No Hit
GTCCGAAACACT825311.2697172640224519No Hit
TACACGATCTAC797351.2267015551348004No Hit
TCCCAGAACAAC750711.1549471680632668No Hit
AGGCTACACGAC739411.1375624216244091No Hit
TATCGTTGACCA739321.1374239590421527No Hit
ATACTTCGCAGG735591.1316854542441934No Hit
AACACAAGGAGT710231.0926697755106154No Hit
AATGTCCGTGAC705221.0849620250983432No Hit
AATCCGTACAGC699251.075777340475336No Hit
AGAGCCTACGTT693161.066408039075987No Hit
GCTGATGAGCTG678561.0439463312877282No Hit
AATGGAGCATGA650731.0011306239077802No Hit
CTAGCGAACATC641200.9864689749199648No Hit
ACACGTAAGCCT569260.8757912175030241No Hit
CCTCTCGTGATC551030.8477448522304245No Hit
ATTAGTTCGCGT550510.8469448461996099No Hit
GTACCTAATTGC522160.8033291327888471No Hit
CAAGCATGCCTA518460.7976367821849734No Hit
CAGCGGTGACAT466920.7183438767461477No Hit
TCACGGGAGTTG462760.7119438284996302No Hit
AGCCTAAGCACG461550.7100822760048499No Hit
GACTTTCCCTCG460330.7082053387787077No Hit
TAATCCACAGCG457530.7038976139973978No Hit
AGCTGGAAGTCC452460.6960975551969546No Hit
AATCTTGCTGCA438350.6743896992454251No Hit
TAACACCACATC436670.6718050643766391No Hit
TCTACGGAGAGC425200.6541587775046304No Hit
CCTGAACTAGTT420730.6472818025858964No Hit
TAACGCTTGGGT407870.6274970380545946No Hit
AGCTTGACAGCT406000.6246200932899341No Hit
ACATTCAGCGCA385680.5933583191627136No Hit
ATCGGCGTTACA364380.5605888413620349No Hit
TACTAATCTGCG356700.5487733676761564No Hit
TGGCAAGACTCT336330.5174346698921268No Hit
CCATAGGGTTCA315670.48564981489860465No Hit
GTCTACACACAT312210.4803266978474145No Hit
TCAACAGCATCG296920.45680344359519015No Hit
CCACCTACTCCA291330.44820337876393224No Hit
CTGCTAACGCAA287240.4419110236369474No Hit
GACACATTTCTG252400.38831061957236296No Hit
AGCTGTTGTTTG240550.37007971290860503No Hit
GACTTGGTATTC233510.359248862029883No Hit
TCGTCGATAATC218370.3359563787480859No Hit
ACCACATACATC201010.30924848510396463No Hit
GTACATACCGGT186540.2869867788234096No Hit
TCTGTTGCTCTC156850.24130951141016294No Hit
ATTGGGCTAGGC152290.23429407390917253No Hit
GATTGGTTGCAC146880.22597093424242737No Hit
GCTCAGTGCAGA115230.17727825948226378No Hit
TCCGCGAAATCT107070.1647243186910178No Hit
CTATTTGCGACA101080.15550886460528704No Hit
CCATGCGATAAC94040.14467801372656502No Hit
TCTCGCGCATCT93040.1431395405903829No Hit
CGACTGTCTTAA78710.12109322054889336No Hit
TCTTCCGCTACT78600.12092398850391334No Hit
TAATGCGCATCT77640.11944705429317852No Hit
TGTTATCGCACA76570.11780088803746366No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGG1303.6416168E-96.00000051
CCCAGTG550.0091587566.00000056
CCGTGCC650.00126160126.00000056
GGATCGA550.0091587566.00000051
CGCTGCA550.0091587566.00000056
GCACCCG650.00126160126.00000054
CCATACG550.0091587566.00000051
CGCGTGC650.00126160126.00000056
GTGACCT550.0091587566.00000056
TGATGCG550.0091587566.00000053
GGCTACC650.00126160126.00000052
CCCTGCG751.7506926E-46.01
GCCCCAG751.7506926E-46.04
CTCACGG100450.06.02
GTCTGCG806.5365384E-56.01
TGGCGCA704.695807E-46.03
GGATCAC600.0033957156.01
CATCTGG704.695807E-46.02
GCTTGTG704.695807E-46.05
GCCGCGG1001.2857527E-66.02
Produced by FastQC (version 0.11.2)