FastQCFastQC Report
Tue 1 Dec 2015
000000000-AKK75_l01n03.3330000003f115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKK75_l01n03.3330000003f115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16665524
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAA244810514.689637121521052No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGATCGT13796918.278713588603635No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACGAA10283266.170379041187064No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAG7251224.351030306637823No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGTTCAAGTTTGGGCAT6242043.7454807901629734No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCG5744963.4472123408780906No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA2525891.515637912135256No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATAA2485801.4915822628799429No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTCACAAGA2309381.3857230051692344No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTTACAAGA2256541.3540168313939605No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA2220861.3326073635608457No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA1883631.1302554903164161No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATAACGAGGTTAAAAAAA1843531.106193840649715No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA1495780.8975295346248938No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC1409040.8454819662436056No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATTCA1364590.8188101376230353No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCGCTGCGATTAAAGCG1200880.7205774027867351No Hit
GAAGACCTTGTTACGACTTCTCCTTCCTACAAACAAAAAGGTTCAGTAGG1196480.717937221775925No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA1042410.625488883517854No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGAACTAGGCGATAGATCAA1002580.6015892449586344No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGTTTCA929960.5580142574574912No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGAAAATTCGATTTGAGCAT921410.5528839057205761No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAA915370.5492596572421006No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGGGTCA821270.49279578607909363No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAG819190.491547700510347No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGTTCTC797940.47879682631041187No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCGA767350.46044156787389345No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAAGCAA750010.45003685452674635No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATAAGGTTTACTTAT721120.43270166602622273No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGTTAAAAAGATTTGAACAA689240.41357235452062596No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATGA602420.36147678284823204No Hit
TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG602000.36122476556992744No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGTAAGGTTTGAACAA599520.3597366635456527No Hit
GGAAACCTTGTTACGACTTCTTCTTCCTCTAGACGTCCAAATTTACAAAG580480.34831188026251075No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGGCCGAGTTCGAAGGT569420.34167542526715633No Hit
GGATACCGTGTTACGACTTCTTCATCCTCTAGATGATAAGGCTCAGGGGA534090.3204759718326288No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTCAATCAA520490.3123154123446703No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG507620.3045928828880508No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCAG495310.2972063764691707No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAACGTAGTTCGATCAA490340.29422417200923295No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGACCAAGTTTGATCAA482070.2892618317911876No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGACTTAGTTCGTTTCA470240.28216334511894137No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCCA439400.2636580763977178No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGTCCCA411770.24707893973210804No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGACGAA383580.23016378002875876No Hit
AGATACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTCAA368120.22088714402259418No Hit
GGTAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGATTTGAACAA348080.20886231960063184No Hit
GGAGACCTTGTTACGACTTCTCCTTCCTACAAACAAGAAGGTTCAGTTAA332330.1994116716642093No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACGAA326610.19597943635015616No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAATCAA287490.17250582699949907No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAGCCAGTTTGGACGT283830.17030967643141612No Hit
GGAAACCTTGTTACGACTTCTCCTTCTTCTAAATACTACAATTTAAAGAA282010.16921760155876286No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCAA276720.16604338393440254No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGACCAAGTTTGACCAA268940.16137506387437922No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGACTAA268530.16112904700746283No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAATTTGTACCA261310.15679674998517898No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAAGCAA255670.1534125179622315No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATGCGCAAGTTTGTATTG248820.14930223616131122No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTAAAGCGA239770.14387186385498588No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAGCGAGTAGATTCACATCA236810.1420957420840773No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGTTCCA234430.14066764417368455No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGATCGA226720.13604132699337868No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGATCAA224750.13485924594990234No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA222710.13363516202670855No Hit
GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT217980.1307969674400877No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCAT217450.13047894563651283No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAGTGAGGTTAAATCAG217200.13032893535180773No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAAATAA216250.12975889626992826No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTACCCAA216180.12971689339021084No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAAAAAAA212290.12738273336019917No Hit
GGAAACCTTGTTACGACTTCTTCTTCCTCTAGTCGTCCAAATTTACAAAA211740.1270527107338479No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGACCAAGTTTGATCGT208830.12530659101988034No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCCAA205630.12338645937565479No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGTTAAGGTTTACACAA204800.12288842523043379No Hit
AGCTACCTTGTTACGACTTTTACTTCCTCTATTGAAGAAATATCGGTGAA202470.12149032937698208No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAGCAAGTTTGATTCA197090.11826210805012793No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATGAGGTTTAAATAA195580.117356045930509No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTACTGAT183750.11025755925826275No Hit
GGAGACCTTGTTACGACTTTTACTTCCTCTAAATGAGCAAGTTTGATTCA174290.10458117008502103No Hit
GGATACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA173360.10402313182591798No Hit
GGAGACCTTGTTACGACTTCTCCTTCCTACAAACAAGAAGGTTCAGTTGA172950.1037771149590016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG15689750.094.9766859
CTTGTTA15087400.094.970727
CCTTGTT15107250.094.964166
TTGTTAC15086500.094.933888
CATGTTA542400.094.75487
AAACCTT13358700.094.740433
GAAACCT13316500.094.732122
AGATACC624250.094.688031
AACCTTG13507450.094.680344
ATGTTAC542800.094.6674658
ACCTTGT15144250.094.601675
ACCATGT544250.094.450165
GGAAACC13287250.094.4054951
GATACCT392100.094.3700562
CCATGTT545000.094.311466
CGTGTTA73100.094.220257
GTTACCT148200.092.692312
GACCTTG484100.092.46854
GTGTTAC74750.092.2675558
GCTACCT428700.092.218922
Produced by FastQC (version 0.11.2)