FastQCFastQC Report
Tue 1 Dec 2015
000000000-AKK75_l01n02.3320000003f118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKK75_l01n02.3320000003f118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16665524
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCGTTGACCA7758924.65567119281698No Hit
AGCAGTCGCGAT6456303.874045604566649No Hit
TAATGACCACGC6111803.667331432242994No Hit
GCGGATGTGACT5652393.391666532657479No Hit
TGAGTCACTGGT5596543.358154235054355No Hit
CCTCGTTCGACT5575973.3458113888288183No Hit
GGAGACAAGGGA5324973.195201062984878No Hit
GACTTGGTATTC5318143.1911027820067344No Hit
TCCGACACAATT5226203.1359349997035797No Hit
GAGTGAGTACAA5016373.010028367544879No Hit
AGTTCAGACGCT5009533.0059240861553467No Hit
TTCAGACTACCA4976712.9862307359792584No Hit
ATTTCGACATGC4886372.932023019498217No Hit
CACGCCATAATG4805252.8833476823171No Hit
TACACGATCTAC4690862.814708976447425No Hit
TGCCCATTTAAG4557622.7347594951109846No Hit
ACCGTGCATAGG4473022.6839960147667723No Hit
CTTATCAAGGCG4429192.657696211652271No Hit
AACGGATAAACG4378022.626992106578827No Hit
TGCCTACAAGCA4186952.512342246184398No Hit
AATCAGTCTCGT4144572.4869125027211867No Hit
CCAGTGTATGCA4071532.443085497941739No Hit
AATCCGTACAGC3926062.355797513477524No Hit
ATCGAATGCGGC3710792.2266266575236395No Hit
GCACACACGTTA3396242.0378837173076585No Hit
TTACTGTGCGAT3304791.983009955162526No Hit
AGGCTACACGAC3290441.9743993648204519No Hit
TGAGAACCTCCA3240001.9441332897783472No Hit
CTAACCTCCGCT3165921.8996822422145263No Hit
CAGGGTGATACC2653191.5920231491071029No Hit
GTATGCGCTGTA2072281.2434532511548992No Hit
ACACCTGGTGAT1918821.1513709379915087No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATCA2050.06.00000053
GTTTCGT1750.06.00000056
CAGTGCA2050.06.00000052
GGTGCTA1750.06.00000054
TCAAGGG2050.06.00000055
GCATCGG1750.06.00000056
AGAGAGT2050.06.00000052
AGGTCAG2000.06.01
ACAACGG1351.375156E-96.05
GTGTTAC1101.8154242E-76.05
CGGGTGT1259.677024E-96.02
AGCGTGA1202.5695044E-86.02
TGTCAAG1259.677024E-96.03
ATGGGAC953.4299337E-66.05
TGATACT1054.830963E-76.06
GCCTTGT550.0091607966.01
GTCGCGC5500.06.05
CTCACGG704.697455E-46.04
CCGGGTG1701.8189894E-126.01
GTACCGC1259.677024E-96.06
Produced by FastQC (version 0.11.2)