FastQCFastQC Report
Tue 1 Dec 2015
000000000-AKK75_l01n01.3310000003f11b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKK75_l01n01.3310000003f11b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16665524
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCTTTGGGGA224680813.481772310309594No Hit
GCTACTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACTCGAGTC11782827.070176731316699No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGAG9657735.79503530762069No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGCTGTCGG5745713.447662371732206No Hit
GCTACTACCGATTGAATGTTTTAGTGAGGTCTTCAGACTGGCCGGCGCAG5710173.4263369096585263No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGACTTTGGGAA5065773.0396703998026102No Hit
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACCGGAAATATGTT2342971.4058783870222142No Hit
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACTGGAGATATGTT2307301.384474919600488No Hit
GCTCCTACCGATGGAACGCAGAGGCAAATTAGGTGGAGACTCGCTCCGGT1071320.642836072841154No Hit
GCTACTACCGATTGGAAGTAGACGAGAGGTCTCGGGATCGATTCCTGGGC1018810.6113279126416907No Hit
GCACCTACCGATTGAATGACTCGGTGAAAAATTGGGACTGTTGACTCGTT948930.5693970378609158No Hit
GCTACTACCGATTGGAAGATTCGATGAGAACTTTGGATTGGGCGCGAAGT907380.5444653285429248No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGACTTTGGGGA893450.5361067554791556No Hit
GCACCTACCGATTGAATGACTCGGTGAAAAATTGGGACTGTTGATTCGTT848930.509392923978868No Hit
GCTACTACCGATCGAATGGCTTAGTGAGGTCTCCGGATTGGCTTTGGGGA813480.48812146560768205No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATCTGAGCA813210.48795945450020056No Hit
GCTACTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACTCGAGGC774190.4645458492634255No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGTTCACGGACTGTTGCCTCTGG721970.43321170099422013No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTTCGGATTGGCTTTGAGGA688800.4133083364195449No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTTCGGATTGGCTTTGGGGA670710.4024535922182825No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG640710.3844523580536682No Hit
GCACCTACCGATTGAATGGTCCGGTGAAATCTTCGGACTGTGACTTTGAC603290.3619988186390059No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGAACTAGGCA598780.35929263310292553No Hit
GCTTCTACCGATTGGACGTCCCGGTGAACAATCGGGAGGCCGTACTCATG584590.35077804934306295No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTTTGGCA575720.3454556844417253No Hit
GCTCCTACCGATTTTGAGTGATCCGGTGAACCTTCTGGACTGTGAGCAGG556070.33366487606390294No Hit
GTTATTTCCGATCGAATGGCTCGGTGAGGGGGTAGGAGCTCCGCGCGCGC548880.32935058027578373No Hit
GCTCCTACCGATGGGACGAGAAGGCAAATTTGATGGACTGGGTCTGCTAA537050.32225209360353746No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACTGCTGTCTTGAG524900.31496159376686866No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCTCACGGATTGTTGCGCTTGG519510.31172737202862627No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCGATGGGGA512630.3075990889935414No Hit
GCTCCTACCGATTTTGAGTGATCCGGTGAACCTTCTGGACTGTGGCCAGG457630.2745968263584151No Hit
GCTGCCCGGAACTGAGCCGTTTCGAGAAACATGGGGACTGTCTGCTTGCG449690.26983249971618056No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTCTCGTA440710.2644441302895727No Hit
GCTACTACCGATTGGATGGCTTAGTGAGATCCTCGGATTGGACTCGAGTC435290.26119190731716563No Hit
GCTACTACCGATGGATGTTTTAGTGAGGTTCACGGACTGACGCGTTTGAT434270.26057986535556876No Hit
GCTACTACCGATTGAACGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGAG433230.2599558225711955No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGATATGGAC415800.24949710552155455No Hit
GCTACTACCGATCGGATGGTTTAGTGAGATCCTCGGATTGGACTCGAGTC403500.2421165995140627No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGTGTTTCA402480.2415045575524658No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGTTTGGAC400980.24060449584423507No Hit
GTTGTTTCCGATGATGGTGCAATACAGGTGATCGGACAGGCGAGTGTTTC387430.23247393841321762No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATGTTG379740.22785962205568813No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCCTCCGGACTGTTGCATTTGG377180.22632351674030773No Hit
GCTCCTACCGATTGAATGATCCGGTGAAATCTCCGGATGGTGGCGTACAT373200.2239353530078022No Hit
GCTCCTACCGATTTCGAGTGATGAGGTGAATCCTCCGGACCGCACACCTC372900.22375534066615604No Hit
GCTCCTACCGATGGTTGAACCGATGAGGACTCGGGATGATCTTATTAGAT371760.22307129376790072No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATTTTGGCA358890.21534876431128117No Hit
GCTACTACCGATCGAATGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGAG357970.21479672646356635No Hit
GCTACTACCGATTGAATGGTTTAGTGAGATCTTTGGACTGACGCACTGCA346750.20806426488600058No Hit
GCTCCTACCGATCGAATGGTCCGGTGAGAATTCGGGATTTTTGGGTTGTT346150.20770424020270828No Hit
GCTCCTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACCGCGCCTAGC324270.19457534008531624No Hit
GCTGCCCGGAACTGAGCCGTTTCGAGAAACATGGGGACCGACTGCTTGTG300160.18010834822835453No Hit
GCTCCTACTGATTGAATGCTCCGGTGAAAAGTCGGGAGAATTCTTGTGCA276670.1660133818774615No Hit
GCTACTACCGATTGAATGAATTAGTGAGGACTTCGGACCGGACATCGCGG273280.1639792424168601No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGACTGGCTCAGAGAG272430.1634692074488627No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGGGCCCGAAGA264380.15863887628135787No Hit
GCTACTACCGATTGAACGGCTTAGTGAGGCTTTCGGATTGGCCCGGGGAG262940.15777481704145638No Hit
GCTACTACCGATTGGATGACTTAGTGAGGTCTTAGGACCGACGTAAAGGA260730.15644872612466312No Hit
GCTTCAACCGATTGGATGATCCGGCAAAGTGGATGGACAAGGTGCTTGCC259560.15574667799224315No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCGCCAGGAG250310.15019629745815374No Hit
GCTCCTACCGATACCGGGTGATACGGTGAACCTTTTGGACCGTATTACGG247080.14825816457976357No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGTCCAGAGAG244550.14674006049854776No Hit
GCTCCTACCGATTGAATGATCCGGTGAAATCTTCGGATGGTGGCATATAG237920.142761787748168No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCCCACGGACTGTTGCTCTTGG233090.1398635890476651No Hit
GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACCGCGCCAGGC232290.13938355613660872No Hit
GCTACTACCGATTGAATGTTTTAGTGAGATCTTCGGCTTGGTTTTCCGAG227560.13654536154998786No Hit
GCTGCCCGGAACTGAGCCGTTTCGAGAAACATGGGGACTGTCTACTTGAG221640.13299311800817065No Hit
GCTGCCCGGGACCGAGCCGTTTCGAGAAAAGCGGGGACTGCTGCTGTCGG220430.13226706823019785No Hit
GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACCGCATGGGCT216230.12974689544715184No Hit
GTTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGTGCCTTTTCA215070.1290508477261201No Hit
GCACCTACCGATTGAATGTTTCGGTGAAACTTTCGGACCGTGGTTTGGAC210720.12644066877225102No Hit
GCTTCTACCGATTGGTCGTCCCGGTGAACAATCGGGAGGCCGTGCTCATG209610.1257746231081603No Hit
GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACTGCGCGGGGC208280.12497656839352907No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGATCCCGT204310.12259440507241177No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCCTCCGGACTGTTGCATTCGG200710.12043425697265805No Hit
GCTGCTACCGATTGAATGCATTAGTGAGTCTTGGGGATCGTTTCTCTTGA193980.11639598010839625No Hit
GCTACTACCGATCGAATGGCTTAGTGAGGTCTCCGGATTGACTTTGGGAA180690.10842143337347208No Hit
GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACCGTTTGGGTT178670.10720935027305471No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGTCCACGGATTGTTGCTCTTGG178370.10702933793140858No Hit
GCTCCTACCGATTGAATGATCCGGTGAAATCTTGGGATCGAGAGGCACAC177090.10626128527371836No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTA174110.10447316268003333No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAATGTTCGGATGTTGGAGACCAC166680.10001485701859719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACC10088500.094.9905853
ACTACCG10091050.094.9891744
CTACTAC10022400.094.943142
GCTACTA9998650.094.930161
CTACCGA13309250.094.927555
TACCGAT13343300.094.878966
ACCGATT12299850.094.840897
CCGATTG11719500.094.822078
CGATTGA8915650.094.795959
TCCTACC2140950.094.722673
GCTCCTA2195350.094.681941
AATCGAC531600.094.633656
CAATCGA542450.094.623475
CCTACCG2787550.094.5654754
CGATTGG2820250.094.5014659
CCGGGAC770500.094.45756
GCACCTA690800.094.298641
TCAATCG541300.094.280434
ACCGATC538800.094.268287
CTCCTAC2210550.094.260812
Produced by FastQC (version 0.11.2)