FastQCFastQC Report
Wed 22 Mar 2017
000000000-AKF34_l01n02_dna_pool.3520000003e98b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKF34_l01n02_dna_pool.3520000003e98b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643739
Sequences flagged as poor quality0
Sequence length26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTACACTGACTTGAGTTAAC390556.066899783918638No Hit
GCCGCCTTACACTGACTTGAGTACGC318134.941909687000477No Hit
GCCGCCTTACACTGACTTGAGTTCGA314324.882724209656398No Hit
GCCGCCTTACACTGACTTGAGTCCTT272634.235101492996385No Hit
GCCGCCTTACACTGACTTGAGTCTAG212343.2985418003259084No Hit
GCCGCCTTACACTGACTTGAGTATCG179482.788086476040756No Hit
CCCGCCTTACACTGACTTGAGTCCTT162932.530994704375531No Hit
GCCGCCTTACACTGACTTGAGTTCTT160242.4892075825761686No Hit
CCCGCCTTACACTGACTTGAGTCTAG122441.9020130829419997No Hit
ACCGCCTTACACTGACTTGAGTACGC92721.4403352911661405No Hit
GCCGCCTTACACTGACTTGAGTTTAG64070.9952791426338935No Hit
GCCGCCTTACACTGACTTGAGTGTCC54030.8393153125723314No Hit
GCCGCCTTACACTGACTTGAGTTGTT49840.7742268217398666No Hit
GCCGCCTTACACTGACTTGAGTTCAT48550.754187644371399No Hit
GCCGCCTTACACTGACTTGAGTTTAC39940.6204377861213939No Hit
GCCGCCTTACACTGACTTGAGTTAGG39520.6139134027921254No Hit
GCCGCCTTACACTGACTTGAGTATGT39330.6109618960479324No Hit
GCCGCCTTACACTGACTTGAGTTTAT36040.5598542266353289No Hit
GCCGCCTTACACTGACTTGAGTACGG33230.5162029953133179No Hit
GCCGCCTTACACTGACTTGAGTTTCT31310.48637724295094753No Hit
GCCGCCTTACACTGACTTGAGTACAC28100.4365123132201094No Hit
GCCGCCTTACACTGACTTGAGTACTT26050.4046671088748701No Hit
GCCGCCTTACACTGACTTGAGTTCGT24970.3878901231710367No Hit
GCCGCCTTACACTGACTTGAGTTCCC24480.3802783426202234No Hit
GCCGCCTTACACTGACTTGAGTTCTA24220.3762394386544857No Hit
GCCGCCTTACACTGACTTGAGTACCC23710.3683169731832311No Hit
GCCGCCTTACACTGACTTGAGTTCCA23260.36132656247330047No Hit
GCCGCCTTACACTGACTTGAGTTCCG23180.3600838227915351No Hit
GCCGCCTTACACTGACTTGAGTCCTC23040.35790902834844557No Hit
GCCGCCTTACACTGACTTGAGTTATC22090.343151494627481No Hit
GCCGCCTTACACTGACTTGAGTTTCG21760.3380251934401986No Hit
GCCGCCTTACACTGACTTGAGTAACG21610.3356950565368884No Hit
GCCGCCTTACACTGACTTGAGTACTC20880.32435505694077876No Hit
GCCGCCTTACACTGACTTGAGTTAGA20520.31876272837283437No Hit
GCCGCCTTACACTGACTTGAGTAAAC20230.31425779702643464No Hit
GCCGCCTTACACTGACTTGAGTACGA19820.30788875615738676No Hit
GCCGCCTTACACTGACTTGAGTTCTC19820.30788875615738676No Hit
GCCGCCTTACACTGACTTGAGTGCTC19020.2954613593397324No Hit
GCCGCCTTACACTGACTTGAGTACCG17800.2765095791928095No Hit
GCCGCCTTACACTGACTTGAGTAACC17510.2720046478464098No Hit
GCCGCCTTACACTGACTTGAGTTATA17490.2716939629259685No Hit
GCCGCCTTACACTGACTTGAGTTAAT17470.2713832780055271No Hit
ACCGCCTTACACTGACTTGAGTATCG17180.2668783466591274No Hit
GCCGCCTTACACTGACTTGAGTATAG16850.26175204547184494No Hit
CCCGCCTTACACTGACTTGAGTCCTC15800.24544108714867363No Hit
GCCGCCTTACACTGACTTGAGTACCT15770.24497505976801157No Hit
GCCGCCTTACACTGACTTGAGTTATG15570.24186821056359797No Hit
GCCGCCTTACACTGACTTGAGTTCCT15180.23580985461499146No Hit
GCCGCCTTACACTGACTTGAGTGTTT15040.23363506017190197No Hit
GCCGCCTTACACTGACTTGAGTCAAC14880.23114958080837109No Hit
GCCGCCTTACACTGACTTGAGTTTGA14590.22664464946197138No Hit
GCCGCCTTACACTGACTTGAGTATGC14450.2244698550188819No Hit
GCCGCCTTACACTGACTTGAGTAGAT14300.22213971811557168No Hit
GCCGCCTTACACTGACTTGAGTACGT14080.21872218399071672No Hit
GCCGCCTTACACTGACTTGAGTTACC13890.21577067724652382No Hit
GCCGCCTTACACTGACTTGAGTTCAC13520.21002300621835868No Hit
GCCGCCTTACACTGACTTGAGTTTTT13160.20443067765041423No Hit
GCCGCCTTACACTGACTTGAGTATTT12640.19635286971893887No Hit
GCCGCCTTACACTGACTTGAGTTCGC12540.19479944511673208No Hit
GCCGCCTTACACTGACTTGAGTCTTT12270.19060519869077375No Hit
GCCGCCTTACACTGACTTGAGTTACA12000.1864109522648154No Hit
GCCGCCTTACACTGACTTGAGTTAAA11950.185634239963712No Hit
GAACGGGAGAGGGGCGGGGAGGGGAA11950.185634239963712No Hit
GCCGCCTTACACTGACTTGAGTATCT11830.18377013044106383No Hit
GCCGCCTTACACTGACTTGAGTCTAT11080.1721194459245129No Hit
GCCGCCTTACACTGACTTGAGTAGAC10870.1688572542598786No Hit
GCCGCCTTACACTGACTTGAGTTCGG10860.16870191179965793No Hit
GCCGCCTTACACTGACTTGAGTTACG10750.16699314473723048No Hit
GCCGCCTTACACTGACTTGAGTTGAT10730.16668245981678909No Hit
GCCGCCTTACACTGACTTGAGTGCTT10680.1659057475156857No Hit
GCCGCCTTACACTGACTTGAGTGCGA10330.16046876140796193No Hit
GCCGCCTTACACTGACTTGAGTTCTG10060.1562745149820036No Hit
GCCGCCTTACACTGACTTGAGTTTGT9930.15425506299913475No Hit
GCCGCCTTACACTGACTTGAGTAACT9860.15316766577758997No Hit
GCCGCCTTACACTGACTTGAGTCTTA9790.15208026855604523No Hit
ACCGCCTTACACTGACTTGAGTACGG9610.149284104272073No Hit
GCCGCCTTACACTGACTTGAGTGAAT9200.14291506340302515No Hit
GCCGCCTTACACTGACTTGAGTATAT9080.141050953880377No Hit
GCCGCCTTACACTGACTTGAGACACT9070.1408956114201563No Hit
GCCGCCTTACACTGACTTGAGTATTC9050.14058492649971494No Hit
GATCGGGAGAGGGGCGGGGAGGGGAA8760.13607999515331523No Hit
GCCGCCTTACACTGACTTGAGTCGTT8750.13592465269309456No Hit
GCCGCCTTACACTGACTTGAGTTGCG8700.13514794039199116No Hit
GCCGCCTTACACTGACTTGAGTTCAA8690.13499259793177049No Hit
GCCGCCTTACACTGACTTGAGTATAC8650.13437122809088778No Hit
GCCGCCTTACACTGACTTGAGTCCAA8600.13359451578978437No Hit
GCCGCCTTACACTGACTTGAGTATTA8450.1312643788864742No Hit
CCCGCCTTACACTGACTTGAGTCTTT8350.12971095428426738No Hit
ACCGCCTTACACTGACTTGAGTACAC8340.1295556118240467No Hit
GCCGCCTTACACTGACTTGAGTCTAC8320.12924492690360534No Hit
GCCGCCTTACACTGACTTGAGTTGGA8230.12784684476161923No Hit
GCCGCCTTACACTGACTTGAGTTTTC8110.12598273523897108No Hit
GCCGCCTTACACTGACTTGAGTCCGT8030.12473999555720564No Hit
CCCGCCTTACACTGACTTGAGTCAAC7980.12396328325610223No Hit
GCCGCCTTACACTGACTTGAGTTTGG7980.12396328325610223No Hit
GCCGCCTTACACTGACTTGAGTTTAA7700.11961369436992322No Hit
CCCGCCTTACACTGACTTGAGTCTTA7670.11914766698926117No Hit
GCCGCCTTACACTGACTTGAGTACAT7660.1189923245290405No Hit
GCCGCCTTACACTGACTTGAGTGAAC7640.11868163960859913No Hit
GCCGCCTTACACTGACTTGAGTTACT6930.10765232493293089No Hit
CCCGCCTTACACTGACTTGAGTCTAT6830.1060989003307241No Hit
CCCGCCTTACACTGACTTGAGTCTAC6660.10345807850697254No Hit
GCCGCCTTACACTGACTTGAGTGCTA6570.10205999636498644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAC250.004481373420.0000025
GCCCCCG305.462972E-420.0000021
CGGGTAG408.241988E-620.00000215
GAGTGAG501.2633245E-720.00000219
AGTCCTA408.241988E-620.00000220
CCTTACC4800.020.0000025
AGTCCGA800.020.00000220
AGTCCCT250.004481373420.00000220
AGTCCCA408.241988E-620.00000220
AGTCCAT501.2633245E-720.00000220
TACCCCG250.004481373420.0000028
AGTTTTG501.2633245E-720.00000220
CTCCGGA800.020.0000022
TTTACAC250.004481373420.0000026
GCGGTGA601.9590516E-920.00000214
AACCCGA250.004481373420.0000022
CGCATTA1000.020.0000023
GGGTAGG408.241988E-620.00000216
ATTACAC800.020.0000026
TCTTACA250.004481373420.0000025
Produced by FastQC (version 0.11.2)