FastQCFastQC Report
Wed 22 Mar 2017
000000000-AKF34_l01n02_cdna_pool.3520000003e998.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKF34_l01n02_cdna_pool.3520000003e998.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758004
Sequences flagged as poor quality0
Sequence length26
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTACACTGACTTGAGTAGAT281853.718318109139266No Hit
GCCGCCTTACACTGACTTGAGTATTG281723.716603078611722No Hit
GCCGCCTTACACTGACTTGAGTAGAA275843.639030928596683No Hit
GCCGCCTTACACTGACTTGAGTTACG250363.3028849451981785No Hit
GCCGCCTTACACTGACTTGAGTGACC233653.082437559696255No Hit
GCCGCCTTACACTGACTTGAGTGCTA211342.78811193608477No Hit
ACCGCCTTACACTGACTTGAGTAGAA167172.2053973329956045No Hit
GCCGCCTTACACTGACTTGAGTTGCA136421.7997266505189946No Hit
ACCGCCTTACACTGACTTGAGTACTC84211.11094400557253No Hit
GCCGCCTTACACTGACTTGAGTCAGA79771.0523691167856635No Hit
GCCGCCTTACACTGACTTGAGTATCC66630.8790191080785853No Hit
ACCGCCTTACACTGACTTGAGTAGAT66120.8722909113936075No Hit
ACCGCCTTACACTGACTTGAGTATTG57420.757515791473396No Hit
CCCGCCTTACACTGACTTGAGTCAGA57230.7550092083946787No Hit
GCCGCCTTACACTGACTTGAGTTATA47990.6331101155139023No Hit
GAACGGGAGAGGGGCGGGGAGGGGAA46330.6112104949314251No Hit
GCCGCCTTACACTGACTTGAGTACTC43240.5704455385459707No Hit
GCCGCCTTACACTGACTTGAGTTTCT38530.5083086632788217No Hit
GAATCGGAGAGGGGCGGGGAGGGGAA36290.47875736803499713No Hit
GCCGCCTTACACTGACTTGAGTTTTG35390.4668840797673891No Hit
GAATGGGAGAGGGGCGGGGAGGGGAA33360.4401032184526731No Hit
GAACCGGAGAGGGGCGGGGAGGGGAA32710.4315280658149561No Hit
GCCGCCTTACACTGACTTGAGTTTGC30640.40421950279945756No Hit
GCCGCCTTACACTGACTTGAGTCACC30620.4039556519490662No Hit
GCCGCCTTACACTGACTTGAGTATCA25840.3408952987055477No Hit
GCCGCCTTACACTGACTTGAGTCAAA23740.3131909594144622No Hit
GCCGCCTTACACTGACTTGAGTATGC21700.2862781726745505No Hit
ACCGCCTTACACTGACTTGAGTATCC21480.28337581332024636No Hit
GCCGCCTTACACTGACTTGAGTGTTA21150.27902227428879No Hit
GAACCGGAGAGCGGCGGGGAGGGGAA20920.2759879895092902No Hit
GCCGCCTTACACTGACTTGAGTGATC19810.26134426731257354No Hit
CCCGCCTTACACTGACTTGAGTCAAA18560.24485358916311786No Hit
GCCGCCTTACACTGACTTGAGTACTA18130.2391807958797051No Hit
GCCGCCTTACACTGACTTGAGTACCT18100.2387850196041182No Hit
GCCGCCTTACACTGACTTGAGGTCCG17370.22915446356483607No Hit
GAATCGGAGAGCGGCGGGGAGGGGAA17210.22704365676170574No Hit
GCCGCCTTACACTGACTTGAGTCTAT16690.22018353465153218No Hit
GCCGCCTTACACTGACTTGAGTTGAA15390.20303322937609827No Hit
GCCGCCTTACACTGACTTGAGTTACC15350.20250552767531568No Hit
GCCGCCTTACACTGACTTGAGTTCCA15060.198679690344642No Hit
GCCGCCTTACACTGACTTGAGTAGCA14270.188257581754186No Hit
GAACGGGAGAGCGGCGGGGAGGGGAA14220.18759795462820775No Hit
GCCGCCTTACACTGACTTGAGTATTC13380.17651621891177355No Hit
GCCGCCTTACACTGACTTGAGTTAAC13090.17269038158109984No Hit
GCCGCCTTACACTGACTTGAGTATAG12980.17123920190394773No Hit
ACCGCCTTACACTGACTTGAGTACTT12630.16662181202210014No Hit
GCCGCCTTACACTGACTTGAGTGTAT12210.16108094416388305No Hit
GCCGCCTTACACTGACTTGAGTTGAC12120.1598936153371222No Hit
GCCGCCTTACACTGACTTGAGTACTT11870.1565954797072311No Hit
GCCGCCTTACACTGACTTGAGTTTTT11850.1563316288568398No Hit
GAACCGGAGAGCGGCGTGGAGGGGAA10840.1430071609120796No Hit
GCCGCCTTACACTGACTTGAGTTTCC10500.1385216964554277No Hit
GCCGCCTTACACTGACTTGAGTCGTG10280.13561933710112348No Hit
GCCGCCTTACACTGACTTGAGTATTA10150.1339043065735801No Hit
GCCGCCTTACACTGACTTGAGTTCGC9630.12704418446340654No Hit
GCCGCCTTACACTGACTTGAGTAATC9260.12216294373116764No Hit
GCCGCCTTACACTGACTTGAGTTCAA9140.12057983862881991No Hit
ACCGCCTTACACTGACTTGAGTATGC9080.11978828607764602No Hit
GCCGCCTTACACTGACTTGAGTTACA8760.11556667247138537No Hit
GCCGCCTTACACTGACTTGAGTGATA8750.11543474704618974No Hit
AGAAACAAGGAGTTTTTTGAACAGAC8540.11266431311708117No Hit
GCCGCCTTACACTGACTTGAGTATTT8260.1089704012116031No Hit
ACCGCCTTACACTGACTTGAGTACTA8080.10659574355808149No Hit
GAATCGGAGAGCGGCGTGGAGGGGAA7640.1007910248494731No Hit
GCCGCCTTACACTGACGTGAGTCACC7600.1002633231486905No Hit
GATCGGGAGAGGGGCGGGGAGGGGAA7600.1002633231486905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAGG1600.020.00000216
CGGCGGT1600.020.00000212
CCTTACC11600.020.0000025
TCCTGGC551.5723344E-820.0000024
ACACCTT408.2451625E-620.0000022
CTGAGTG551.5723344E-820.00000217
GGACTCT551.5723344E-820.00000213
GAATCCG408.2451625E-620.0000021
TCCTAAA408.2451625E-620.0000024
AGCGGCC7650.020.00000210
TGTCTTG551.5723344E-820.00000213
ACGGCCC408.2451625E-620.00000211
GAGGTCC1600.020.00000219
CAGGATC551.5723344E-820.00000213
GTCTTGA551.5723344E-820.00000214
TGAGCCA800.020.00000218
CCCATTA408.2451625E-620.0000023
GAGATAC1100.020.00000219
GTGCCGG551.5723344E-820.0000025
TCAGTCT408.2451625E-620.00000218
Produced by FastQC (version 0.11.2)