FastQCFastQC Report
Wed 22 Mar 2017
000000000-AKF34_l01n01_dna_pool.3510000003e98e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKF34_l01n01_dna_pool.3510000003e98e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643739
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTC339065.267041456242359Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCTTACACTGACTTGAGTCCTTA181932.826145378794822No Hit
CCGCCTTACACTGACTTGAGTTAACC145292.2569706045462525No Hit
ACACTCTTTCCCTACACGACGCTCTT130212.022714174533468Illumina Single End PCR Primer 1 (100% over 26bp)
CCGCCTTACACTGACTTGAGTACGCA128982.0036070519263243No Hit
CCGCCTTACACTGACTTGAGTCTAGG124201.9293533559408393No Hit
CCGCCTTACACTGACTTGAGTTCGAG120501.871876645659188No Hit
CCGCCTTACACTGACTTGAGTATCGA73991.1493788631728075No Hit
CCGCCTTACACTGACTTGAGTTCTTA33020.5129408036486838No Hit
ACGCTGATCCACGTGGCCACGTCTCA30550.4745712159741759No Hit
TCATCCCTTGTTGTTCTTCTTTCAAC19950.3099082081402556No Hit
CCTCCCGCTTTTAAAAACACGGCGAG17860.2774416339541336No Hit
CCGCCTTACACTGACTTGAGTGTCCA17820.27682026411325084No Hit
GCCGCCTTACACTGACTTGAGTCCTT17540.2724706752270718No Hit
CCGCCTTACACTGACTTGAGTACGCT17420.2706065657044237No Hit
CCGCCTTACACTGACTTGAGTATGTT14710.22850875898461956No Hit
GCCGCCTTACACTGACTTGAGTTAAC14650.22757670422329546No Hit
CCGCCTTACACTGACTTGAGTCCTCC13200.20505204749129693No Hit
CCGCCTTACACTGACTTGAGTTGTTA12900.20039177368467656No Hit
CCGCCTTACACTGACTTGAGTTCTTT12450.19340136297474597No Hit
CCGCCTTACACTGACTTGAGTTTAGG11920.18516821258304997No Hit
CCGCCTTACACTGACTTGAGTTTATG10630.1651290352145823No Hit
CCGCCTTACACTGACTTGAGTTAGGT10550.16388629553281686No Hit
CCGCCTTACACTGACTTGAGTTCATC9800.1522356110162659No Hit
GCCGCCTTACACTGACTTGAGTCTAG9710.15083752887427979No Hit
CCGCCTTACACTGACTTGAGTACGGT9610.149284104272073No Hit
CCTTATGCTGCCAGGGAAAAGCAATT9410.1461772550676594No Hit
GCCGCCTTACACTGACTTGAGTTCGA9020.1401188991190529No Hit
GCCGCCTTACACTGACTTGAGTACGC8740.1357693102328739No Hit
CCGCCTTACACTGACTTGAGTTCTTC8160.12675944754007448No Hit
CGCCTTACACTGACTTGAGTCCTTAT7310.11355533842131672No Hit
CCGCCTTACACTGACTTGAGTTATCC7070.10982711937602041No Hit
GCCTTACACTGACTTGAGTCCTTATG6740.10470081818873798No Hit
CCGCCTTACACTGACTTGAGTTCTAT6600.10252602374564847No Hit
CGGAAGAGCACACGTCTGAACTCCAG6550.10174931144454506Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCTA250.004481373420.00000219
ATGTGGG250.004481373420.0000025
GACCGCC501.2633245E-720.00000219
GGATCAT250.004481373420.00000213
CCAGCGG250.004481373420.0000024
GTTAGGG250.004481373420.00000220
AGCGAGA250.004481373420.0000025
GCAGCGG250.004481373420.0000024
GGGATCA250.004481373420.00000212
GAGGCCC250.004481373420.00000219
GGCCACT356.694748E-520.015
CGCTCAT950.020.02
CACGTGC652.4556357E-1020.010
CACTCTT15750.019.8095232
ACACTCT15700.019.8089181
GATCGGA37200.019.7580661
TTTCCCT15850.019.621457
CTCTTTC15800.019.6202534
ATCGGAA37000.019.5135142
TTCCCTA15950.019.4984328
Produced by FastQC (version 0.11.2)