FastQCFastQC Report
Wed 22 Mar 2017
000000000-AKF34_l01n01_cdna_pool.3510000003e99b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKF34_l01n01_cdna_pool.3510000003e99b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758004
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTC16529521.806613157714207Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCTTACACTGACTTGAGTAGAAA159692.1067171149492614No Hit
CCGCCTTACACTGACTTGAGTAGATC143171.8887763125260553No Hit
GCCGCCTTACACTGACTTGAGTAGAA141531.8671405427939693No Hit
CCGCCTTACACTGACTTGAGTATTGC140441.8527606714476441No Hit
ACACTCTTTCCCTACACGACGCTCTT128691.6977482968427609Illumina Single End PCR Primer 1 (100% over 26bp)
CCGCCTTACACTGACTTGAGTGACCG108621.4329739684751004No Hit
CCGCCTTACACTGACTTGAGTTACGC90311.1914185149418737No Hit
CCGCCTTACACTGACTTGAGTGCTAT81641.0770391712972491No Hit
GCCGCCTTACACTGACTTGAGTATTG77321.0200473876127303No Hit
TACGCCACTGAAATCAGAGCATCCGT68580.9047445659917361No Hit
GACCGTGTGGCTGTATTAAAATACAA56790.7492044896860702No Hit
CCGCCTTACACTGACTTGAGTTGCAC52150.6879910923952908No Hit
GACCGTGGCCTGGTCTGACTCACATG51050.6734792956237698No Hit
GCCGCCTTACACTGACTTGAGTAGAT47490.62651384425412No Hit
GCTATATGGGCAATCTTCAAACATTG30290.39960211291760994No Hit
GCCGCCTTACACTGACTTGAGTGCTA29700.3918185128310669No Hit
GCCGCCTTACACTGACTTGAGTTACG28200.37202969905172006No Hit
CCGCCTTACACTGACTTGAGTACTCA28090.3705785193745679No Hit
CCGCCTTACACTGACTTGAGTCACCG26600.3509216310204168No Hit
GCCGCCTTACACTGACTTGAGTTGCA26000.34300610550867805No Hit
GATCGGACGAGCACACGTCTGAACTC24300.32057878322541833Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCGCCTTACACTGACTTGAGTATCCC24020.31688487131994025No Hit
GCCGCCTTACACTGACTTGAGTGACC22550.2974918338161804No Hit
CCGCCTTACACTGACTTGAGTCAGAC22490.2967002812650065No Hit
CACCGAAAACCCCACTGCAGATGTAT21170.27928612513918133No Hit
TGCACTATGACTGGACTCTAAATAGA21110.2784945725880074No Hit
GCCGCCTTACACTGACTTGAGTATAG20940.2762518403596815No Hit
GCCGCCTTACACTGACTTGAGGTCCG19910.26266352156453004No Hit
GCCGCCTTACACTGACTTGAGTTTGC19820.2614761927377692No Hit
GCTATATGAGCAATCTTCAAACATTG18860.24881135191898723No Hit
CCGCCTTACACTGACTTGAGTCAAAT18260.2408958264072485No Hit
GCCGCCTTACACTGACTTGAGTACCT16200.21371918881694557No Hit
GCCGCCTTACACTGACTTGAGTGATC16060.21187223286420653No Hit
GCTATATGGCCGCAAAACGAGATTCC16060.21187223286420653No Hit
GACCGTGAGGCTGGCTAACAATCGGA15890.20962950063588057No Hit
CAGACCAGCCGTTGCATCGTCACCAT14570.19221534451005537No Hit
CCGCCTTACACTGACTTGAGTTTGCT13540.17862702571490388No Hit
CCGCCTTACACTGACTTGAGTTTTGC13320.1757246663605997No Hit
CACCCAGGCATTCCATAAATAAAATC12820.16912839510081742No Hit
GCCGCCTTACACTGACTTGAGTATGC12680.16728143914807836No Hit
CCGCCTTACACTGACTTGAGTACTAT12650.16688566287249143No Hit
GACCGTGCGCCCACACCATCATTTAT12630.16662181202210014No Hit
CCGCCTTACACTGACTTGAGTATGCT12300.16226827299064386No Hit
CCGCCTTACACTGACTTGAGTCTATA12040.15883821193555708No Hit
ACTATCCCTGATACCGGCAAAGTGAT11780.1554081508804703No Hit
CCGCCTTACACTGACTTGAGTATCAC11690.1542208220537095No Hit
CCGCCTTACACTGACTTGAGTACCTG11670.1539569712033182No Hit
AGAAACAAGGAGTTTTTTGAACAGAC11200.14775647621912286No Hit
CCGCCTTACACTGACTTGAGTTATAT10830.14287523548688397No Hit
CCGCCTTACACTGACTTGAGTTTCTC10350.136542815077493No Hit
TGCCGCCTTACACTGACTTGAGTAGA9840.12981461839251507No Hit
CCGCCTTACACTGACTTGAGTGTTAT9420.12427375053429797No Hit
GACCGTGTAGCTGTATTAAAATACAA9360.1234821979831241No Hit
ATTGCTATTGCGAACTGACAGATTCA9310.12282257085714587No Hit
GATCGGAAGAGCACACGTCAGAACTC9120.12031598777842861Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TACGCCACTGAAATCAGAGCATCCGG9050.1193925098020591No Hit
GATCGGAAGAGCACACGTCTGAACCC8420.11108120801473344Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGAAGAGCACACGTCTGAACTCCAG8390.1106854317391465Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTTACACTGACTTGAGTAGAAACAA8320.10976195376277698No Hit
CCGCCTTACACTGACTTGAGTATTGT8280.10923425206199439No Hit
CGCCTTACACTGACTTGAGTAGATCA8050.10619996728249456No Hit
CCGCCTTACACTGACTTGAGTAGCAA7980.10527648930612503No Hit
CCGCCTTACACTGACTTGAGTATAGC7880.10395723505416858No Hit
CCGCCTTACACTGACTTGAGGTCCGT7650.10092295027466873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAAAT408.2451625E-620.00000216
CGTGCAC551.5723344E-820.0000024
TCCGAAC800.020.00000218
CGCATTA551.5723344E-820.0000023
ATATGCG800.020.0000024
CCTAATA551.5723344E-820.00000216
AGGAAAA408.2451625E-620.00000220
GTCCGAA800.020.00000217
ACGTCCG800.020.00000215
TAGATCC800.020.00000218
CACGTCC800.020.00000214
CGCCGTA551.5723344E-820.0000023
ATCGGAG551.5723344E-820.0000022
GTGCGGC408.2451625E-620.0000025
CAGAGGG551.5723344E-820.0000023
CCGCATT551.5723344E-820.0000022
GCCGTAC551.5723344E-820.0000024
CTCAGAG408.2451625E-620.00000213
CGTGGCT250.004482095620.06
AACCGGC305.464202E-420.012
Produced by FastQC (version 0.11.2)