FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKDB2_l01n02_e2_mcb15.3520000003cb22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKDB2_l01n02_e2_mcb15.3520000003cb22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4545630
Sequences flagged as poor quality0
Sequence length301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG123070.2707435492989971Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG31100.025.136654295
TCTCGGG37750.023.834436295
TCGGAAA79750.015.721003295
GTCGGGG17101.2732926E-1114.663743295
AGCGTCC30100.014.210965295
GAGCGTT36650.013.281037295
GTCGCCC19158.185452E-1113.093995295
CGCCGTT42200.010.835308295
TCGCCGG52700.010.6356735295
GTAGATT99500.010.228643295
GCGTCGG53900.010.125232295
CGGTGGG50250.09.686567295
TCGCGGG14000.00113372069.482143295
TCGTGTT67150.09.445272295
AGTGTAA86100.09.422184295
CGTCGTT61950.09.04762295
GAGTGTT55600.09.019784295
GTGGTCC29451.0293661E-89.01528295
GAGCGGG15000.00212611338.849999295
AGAGCGC49300.08.676471295
Produced by FastQC (version 0.11.2)