FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKDB2_l01n02_e16_mcb15.3520000003cb3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKDB2_l01n02_e16_mcb15.3520000003cb3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4426429
Sequences flagged as poor quality0
Sequence length301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG525841.1879553473014024Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG30550.024.14075295
TCTCGGG33350.015.479761295
CGGTGGG47700.015.151992295
GAGCGTC1175050.014.9125579
AGAGCGT1193650.014.7419688
TCGGAAA76050.014.546351295
AAGAGCG1233500.014.4929067
GAAGAGC1273800.014.0807046
CGGAAGA1286850.013.8920614
TCGGAAG1289750.013.8036433
ATCGGAA1305900.013.6781152
GATCGGA1306700.013.6697411
GGAAGAG1340700.013.4440975
GTAGATT88600.011.154064295
TCGCGGG14801.413688E-510.962838295
GAGCGTT35150.010.910384295
CGCCGTT42600.010.733568295
GCGTCGG49750.010.080402295
GTCGACC19402.5596655E-69.88402295
GTCGGGG18001.1309325E-59.833333295
Produced by FastQC (version 0.11.2)