FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKDB2_l01n02_d14_mcb15.3520000003cb15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKDB2_l01n02_d14_mcb15.3520000003cb15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4070874
Sequences flagged as poor quality0
Sequence length301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG349020.8573588865683389Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG29250.018.658121295
GAGCGTC661100.017.3805039
AGAGCGT673100.017.2021248
AAGAGCG701300.016.6155727
TCTCGGG27900.015.860215295
GAAGAGC745050.015.798276
TCGGAAG742250.015.7982493
CGGAAGA744700.015.6670484
GATCGGA747850.015.5418871
ATCGGAA756350.015.4257292
CGGTGGG37500.014.946668295
GGAAGAG797700.014.7925285
GAGCGTT30450.013.078818295
TCGGAAA64600.012.558051295
GTGGTCC23402.3101165E-910.715813295
ACCCGCC18151.0898093E-610.564738295
TCGTGTT54350.010.312788295
TACCCCC25057.0886017E-910.00998295
GTAGATT82100.09.881243295
GCGTCGG39450.09.721166295
Produced by FastQC (version 0.11.2)