FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKDB2_l01n02_d13_mcb15.3520000003cb08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKDB2_l01n02_d13_mcb15.3520000003cb08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4298727
Sequences flagged as poor quality0
Sequence length301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG305060.7096519504495168Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC566900.017.3024359
AGAGCGT579200.017.036868
AAGAGCG608800.016.402357
GTAGGGG31500.015.920635295
GAAGAGC655100.015.5583116
TCGGAAG647600.015.3968513
CGGAAGA651550.015.3261454
GATCGGA650950.015.2949541
ATCGGAA663350.015.1424592
GGAAGAG704950.014.3953485
TCTCGGG27600.013.894928295
CGGTGGG39450.013.086185295
TCGGAAA71450.012.79916295
TCGTGTT61050.010.389025295
TCGCCGG39400.09.359137295
GAGCGTT31603.6925485E-109.3354435295
CGTCGTT52800.09.21875295
AGCGTCC24304.9074515E-79.1049385295
GTGGTCC26251.5508886E-78.990477295
CACGCCC28355.3269105E-88.844796295
Produced by FastQC (version 0.11.2)