FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKDB2_l01n01_e2_mcb15.3510000003cb25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKDB2_l01n01_e2_mcb15.3510000003cb25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4545630
Sequences flagged as poor quality0
Sequence length301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC111360.2449825436738142TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAA86550.018.575968295
ACACGTT53600.016.511194295
TATGCCC38800.015.966494295
CCAGTCC50400.013.169642295
GCACACC40200.011.741293295
TCACCAA155150.010.362552295
AGAGCAA137600.09.647529295
AGCACAA121350.09.237742295
CGTCTTT101100.08.899604295
ACGTCTT89650.08.555494295
CTGAACC67700.08.279173295
TCTCGTT77100.08.22633295
GTCACCC38851.7744242E-87.5933075295
TCGTATT110800.07.0555058295
ACCAGAA138700.06.9124007295
GAGCACC43401.355329E-76.7972345295
CGATCGG25600.0051879856.3378906295
GCACACA54703.468449E-86.202011295
AACTCCC56206.025584E-86.036477295
CCAGATA90550.06.027057295
Produced by FastQC (version 0.11.2)