FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKDB2_l01n01_e16_mcb15.3510000003cb32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKDB2_l01n01_e16_mcb15.3510000003cb32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4426429
Sequences flagged as poor quality0
Sequence length301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC479321.0828593432764877TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT52150.015.838926295
TCGGAAA79500.014.657232295
CCAGTCC47250.013.42328295
AGAGCAC1418300.012.4901298
GAGCACA1420700.012.4067369
TCGGAAG1508800.012.0342323
GAAGAGC1494100.012.0242966
CGGAAGA1511450.011.9643384
GATCGGA1532950.011.8542681
ATCGGAA1545700.011.7755722
AAGAGCA1523250.011.7457747
GGAAGAG1551500.011.6174675
TATGCCC34750.011.460431295
CACCTTT139650.011.090226295
CGTCTTT88850.010.790658295
TCGTATT101650.010.59272295
TCTCGTT76550.010.4049635295
GCACACT48200.09.792531295
ACGTCTT80950.08.92835295
AGAGCAA131250.08.878096295
Produced by FastQC (version 0.11.2)