FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKDB2_l01n01_d14_mcb15.3510000003cb18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKDB2_l01n01_d14_mcb15.3510000003cb18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4070874
Sequences flagged as poor quality0
Sequence length301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC322560.7923605594277788TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC51000.019.088236295
TCGGAAA70000.016.435715295
AGAGCAC848950.013.7605288
GAGCACA850350.013.6858369
TCGGAAG914500.012.9677433
GATCGGA927200.012.8060291
CGGAAGA921450.012.7898974
GAAGAGC917650.012.7624916
ATCGGAA947200.012.597922
AAGAGCA944400.012.4478517
GGAAGAG964650.012.2324175
CGCCAAA65750.010.768062295
AGAGCAA122050.09.5473175295
ACACGTT47700.09.27673295
CGTCTTT82000.08.454268295
CCAGTCC43253.092282E-118.184972295
ACGTCTT68850.07.926652295
CGGACGA29106.4756186E-67.6030936295
GCACACT38308.602583E-76.932115295
TCGTATT86300.06.8366165295
Produced by FastQC (version 0.11.2)