FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKDB2_l01n01_d13_mcb15.3510000003cb0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKDB2_l01n01_d13_mcb15.3510000003cb0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4298727
Sequences flagged as poor quality0
Sequence length301
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC266550.6200672896883194TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT47700.015.151991295
GAGCACA752000.013.2396949
AGAGCAC755650.013.1562228
TCGGAAA72750.012.773196295
TCGGAAG811550.012.4681173
CGGAAGA818850.012.2849114
GATCGGA829700.012.23092651
GAAGAGC824200.012.1156886
ATCGGAA847100.012.0319332
AAGAGCA849050.011.8305757
GGAAGAG877700.011.5284265
ACGTCTT74100.010.350877295
CCAGTCC39750.010.0188675295
CGTCTTT85050.09.365079295
TATGCCC33951.4915713E-109.123712295
AGAGCAA125800.08.79372295
GCGCGCA16500.0050315868.0454545
CAGTGAA142050.07.372404295
CACACAA132500.07.013207295
AACTCCC51551.4224497E-96.867119295
Produced by FastQC (version 0.11.2)