FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKBTW_l01n03.3330000003c56a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKBTW_l01n03.3330000003c56a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19026253
Sequences flagged as poor quality0
Sequence length251
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATAGACTTAGTA16991188.930386871235235No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGTATAGTC16203598.51643778730368No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG4905362.578206018809904No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGTGTCAGTTATAGTTTAGTG4736692.4895548272168986No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA3157891.6597540251356901No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGTGTCAGTTAAGATCCAGGT2933921.5420377307081956No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGTCTGGTC2826301.48547378193699No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATCCAGCTTAGAA2549831.3401640354514366No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGTT2326541.2228051419267894No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2208581.1608065970740533No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATATAGCTTTGTG2073821.0899781475627388No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTGAGCGTCAGTTACAGTTTAGTA1803480.9478902650984407No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTGAGCGTCAGTTATAGTTTAGTA1704840.8960461106030705No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT1683030.8845830022338081No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGTATAGTA1640690.8623295401359374No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA1505040.7910333159135432No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTATAGTTTAGTA1479050.7773732431708966No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGGATAGTC1427570.7503158924671085No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGTTGTCACCCAGCA1350350.7097298664114263No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTAGCTTAGTA1342050.7053674730384379No Hit
CCTGTTTGATACCCACACTTTCGTGCTTCAGTGTCAGTTGTGGCCTGGTA1311230.6891688027064499No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTG1180590.6205057821947391No Hit
CCTGTTTGATTCCCACGCTTTCGTGCTTCAGCGTCAGTGACAGTTTAGTA1101670.5790262538819388No Hit
CCTGTTTGCTACCCACGCTTTCGCATCTGAGCGTCAGTCATCGTCCAGAA1054380.5541711234471653No Hit
CCTGTTTGTGTTCTGTGCCTTCGTCCCTCAACGTCAGTTATTACATAAAG922710.48496674568555354No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTATAGACTTAGTA903260.47474402868499643No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT894310.4700400020960511No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATGGTCCAGCC866660.4555074506788068No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTATAGTGCCAGAT844480.4438498741712307No Hit
CCTGTTTGATCCCCACGCTTTCGCATCTCAGCGTCAGTACTGCACCAGGT835230.4389881707133822No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGGATAGTC814640.428166281610993No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG810180.42582215215996555No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA738380.3880848215358011No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGTCTAGAA707030.3716075887354173No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGTCCAGTT676180.3553931507165389No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA633490.33295573227161435No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGTTTAGTG620250.3259969264573534No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG608400.31976869013567727No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGTAGCTTAGTA575270.30235590791313455No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATATGGCTTTGTG571580.3004164824256253No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTACACTCCAGTG550330.289247704211649No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTAATAGTCCAGTT528700.27787920196372873No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGTGTCAGTTGCGACCTGGTA514180.27024764150881414No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGTATAGTC489180.25710790243354803No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT487280.2561092822638278No Hit
CCTGTTTGATACCCACCCTTTCGTGCATCAGCGTCAGTTATAGTATAGTC458390.2409249997884502No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAATGTCCAGCA455470.23939027826445913No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGTGTCAGTTACAGTTTAGTA430610.22632412172801444No Hit
CCTGTTTGATACCCACGCTTTCGTGCCTCAGCGTCAGTTATACTATAGTA429150.2255567609660189No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGTGCCAGGT426580.22420599578908154No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTACATCCCAGAT409030.21498189895824468No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA395430.2078338809012999No Hit
CCTGTTCGCTACCCATGCTTTCGTGCTTCAGCGTCAGTTATAGCTTGGTA392750.20642530087243136No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATAGTATTAGTC391350.20568947548421646No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATAGACTTAGTA390210.20509030338238432No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCAGTCCAGGT370940.19496219250316918No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTTGTGCCAGGT370690.19483079511241652No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGTCTAGTC368980.19393203695966832No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGAAATAGCTTTGTC351480.184734219606982No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATAATGAATTAGTC345070.18136519050808375No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT343180.18037182623399364No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTATAGTATAGTC342770.18015633451315927No Hit
CCTGTTTGCTCCCCATGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAAGT339760.1785743099284972No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG336460.1768398643705621No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTTTAGTGCCAGAT330470.1736915828881283No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA323280.16991259393008176No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATACCTTAGTA319800.1680835422508047No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTAATGGTTTAGTA316320.16625449057152766No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGAAGTAACCCAGCA312740.16437287993594957No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG311960.16396292007680124No Hit
CCTGTTTGATACCCACGCTTTCGTGCATCAGCGTCAGTTATAGTATAGTC310610.16325337416673688No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTCTTGGTCCAGAT306740.16121934255788567No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAACATAGAA304360.15996843939792033No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGTCCAGAA297570.15639968626507805No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA273110.14354376555383763No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCAATGTAGATTCGTC270850.14235593314143358No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG263620.1385559206008666No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGTATAGTA257830.13551275703103496No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGAAATAACCCAGCA254810.1339254765507428No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCAGTCCAGTT251690.1322856371141496No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAACGTCAGTTACAGTCCAGAA247530.13009918453202532No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTTAAGATCCAGGT245350.1289533992846621No Hit
CCTGTTTGCTCCCCACACTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG238740.1254792522731617No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA237210.12467510024175543No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATGGTTTAGTA236790.12445435262529096No Hit
CCTGTTTGCTACCCACACTTTCGTGCATCAGCGTCAGTTATAGTATAGTC235930.12400234560110181No Hit
CCTGTTTGATCCCCACGCTTTCGCATCTCAGCGTCAGTACTGCACCAGAT228760.12023386843431547No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTAAGGGCTAGTA224950.1182313721992449No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAGTTATAGTATAGTC219860.11555612132352071No Hit
CCTGTTTGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGTACTATAGTA215710.11337492463702654No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAATGCCCAGCA210250.1105052056229884No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGCA203680.10705208219400845No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACGTCCCAGCA201590.1059536000073162No Hit
CCTGTTTGCTCCCCACACTTTCGTGCATCAACGTCAGTTTCTGTCCAGAA200080.10515995976717013No Hit
CCCGTTCGCTACCCTGGCTTTCGCGCCTGAGCGTCAGACACAGTCCAGAA199170.10468167326483044No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGAAGTGACCCAGCA196520.10328886092285222No Hit
CCTGTTTGATACCCACGCTTTCGTGCCTCAGCGTCAGTGACATTCTAGTA194960.10246894120455562No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATCAGCGTCAGTTAAAGCCCAGCA191580.10069244848157964No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTGAGCGTCAGTTATAGTTTAGTA191210.10049798034326571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC8099550.0244.835133
TGTTTGA8166650.0244.7873
TTGATAC6426550.0244.738866
TTTGCTC7132100.0244.458975
CTGTTTG16400850.0244.400222
CTGTTCG736350.0244.051732
GTTTGCT8138550.0243.628774
CCTGTTT16302100.0243.238631
TTTGATA6436750.0243.23585
TGATACC6472600.0242.573687
CCTGTTC738150.0241.631121
GTTTGAT8276300.0241.512824
CTCCCCA7458300.0241.483479
TTGCTCC7252100.0241.373356
GATACCC6549700.0240.298038
TGTTCGA48950.0239.994893
TGATCCC1674100.0238.729027
GCTCCCC7888150.0238.558298
TGCTCCC7351350.0238.127917
ATACCCA6620300.0237.01389
Produced by FastQC (version 0.11.2)