FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKBTW_l01n02.3320000003c56d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKBTW_l01n02.3320000003c56d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19026253
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGTACATAA4409952.3178236933988No Hit
CGGAGAAGGGAA4366352.2949079884515355No Hit
ACGTGGTTACGT4209512.212474521388946No Hit
TGCACCTCTGTC4143402.1777277953783125No Hit
CGCACCAAGTGA4134722.17316567797138No Hit
CAGGTCTGTATT4133492.1725192028088767No Hit
TTATCCGAGGTT4121952.166453899251734No Hit
CGTCGTCAAATG3990622.097428221941546No Hit
TGGGATGCATGC3971652.0874577879312337No Hit
TTGTCTGGAAGC3962602.0827012023859877No Hit
CAGTTGGTCTGA3943222.0725152766548414No Hit
GCACTCGTTAGA3926212.06357499818803No Hit
CTCGATTAGATC3877532.0379892982606718No Hit
AGTCACATCACT3875872.037116819586074No Hit
TTACCAAGACTC3842122.019378171834465No Hit
GTAGTAGGCCAC3813682.004430404662442No Hit
CGGCGCAAGATT3798231.9963100459139276No Hit
GATGGGATTTCC3751911.9719647373552744No Hit
GAGTCGCGTTTA3727081.9589143485057199No Hit
ATTGCCCAGATG3714571.9523392230724568No Hit
TCAGGTTGCGGA3684251.9364033475219742No Hit
GCGCCGTATAAT3656081.921597489531964No Hit
GGAATAAACGTG3575741.879371624039689No Hit
GCTACTTCTTCC3568681.8756609617248337No Hit
CAATCTGTTGGT3556411.8692119777866931No Hit
ATGCCAACCAAC3543491.8624213606325954No Hit
ACAATTTCCGAC3451961.8143141479302307No Hit
CATGAGTGCTAC3449111.8128162176756506No Hit
GTTTGGCGACGA3422641.7989038619427589No Hit
TAGTACCCGAGG3421711.798415063649159No Hit
ATTACGGCGGCA3337041.7539133953490476No Hit
TCTAGGAGTTTC3328141.7492356482382527No Hit
GTGGACGTCCAA3187371.675248405453244No Hit
TCAGGACTGTGT3175751.6691410547310603No Hit
AAGCCGTTCCAA3143071.6519647878118722No Hit
GCTATGGACCGA2919031.534211702114967No Hit
GCATGCTGGGAT2846741.4962168326049277No Hit
ATTCAGCACCAG2831491.4882015917690152No Hit
GGACCGAACTCA2782261.462326817582001No Hit
CTCGCTTCACTT2527411.3283803174487379No Hit
GTATTGACGGTC2440531.2827170962143728No Hit
ACTGGTTGAGTC2374171.2478389728129864No Hit
GACTAACGTCAC1414810.7436093696430927No Hit
TGCCATCTGAAT546970.28748172327993327No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAG909.152396E-66.00002671
GCAGTCG550.0091605446.0000261
TCTAGAG751.7513447E-46.0000261
TCAGGGT2950.06.0000261
ACAGGTT650.00126195136.0000261
GTATCTG550.0091605446.0000261
GAGCCCC550.0091605446.0000261
CGGAGCA4800.06.0000261
AAGTCGT1001.2866221E-66.00002571
ACAACCG704.697641E-45.99999484
CGTGGTG852.44516E-55.99999482
TCCGCTG1001.2867422E-65.99999484
CGTGCTA2850.05.99999485
CCTAACG1001.2867422E-65.99999482
CCTCGGT1552.7284841E-115.99999485
GCGCAAT1351.375156E-95.99999483
CCAGAGG2550.05.99999486
TTGAGCC1054.831345E-75.99999486
GTCAACC1950.05.99999483
AAGGGAC2300.05.99999486
Produced by FastQC (version 0.11.2)