FastQCFastQC Report
Mon 16 Nov 2015
000000000-AKBTW_l01n01.3310000003c560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKBTW_l01n01.3310000003c560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19026253
Sequences flagged as poor quality0
Sequence length251
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA460302924.19304000635333No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA198841110.450880685755624No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA9051174.757200485035072No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA7866584.134592344588291No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA6456223.3933218485005954No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5559272.921894289958196No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCGCGTA5229762.748707273050558No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3564901.8736742331766536No Hit
TACAGAGGGTGCAAGCGTTAATCAGAATGACTGGGCGTAAAGGGCGTGTA3448241.8123589547558314No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA3421491.7982994339452965No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCGCGTA2751251.4460282852330408No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA2661951.3990931372561901No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2585291.358801441355794No Hit
TACGAGATGTGCGAGCGTTGTTCGGAATCACTGGGCATAAAGGGTGCGTA2575281.3535402898300575No Hit
TACGGAGGGTCTAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2237501.1760066472363213No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1690780.8886563213471408No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1425850.7494118784187302No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCATGTA1329320.6986767179013125No Hit
TACGGAGGGTCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1301500.6840548162583563No Hit
AACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1274850.6700478544041225No Hit
TACGTAAAAGACAAGTGTTATTCATGTTAAGTAGGTTTAAAGGGTACCTA1094970.5755048038097674No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1000870.5260468259304657No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA990210.5204440411887721No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA828740.4355770944494431No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA781480.4107377317015599No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA702220.3690795029373361No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGCGCGCGTA673540.3540055942701908No Hit
TACGGGGGGTCCAAGCGTTATTCGGAATCATTGGGTGTAAAGCGCATGTA568730.2989185521710449No Hit
TACGGGGGGTCCAAGCGTTATTCGGAATCATTGGGCGTAAAGCGCATGTA549200.288653788005447No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA507360.26666312068908155No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA495910.26064512019260966No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA474280.24927661794468936No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA427740.22481567968217392No Hit
TACGGAGGATGCGAGCGTTATTCGGATTTATTGGGTTTAAAGGGTGCGTA413340.21724718997482056No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA404450.21257469875965593No Hit
TACGGGGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGCGCGTA403590.21212269173546677No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA402700.2116549170243873No Hit
TACGTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA399190.20981009765821995No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGTA372710.19589248602969803No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA344420.18102355729212682No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA336210.17670846697980944No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA324980.17080609618719986No Hit
TACGGGGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA323310.16992836161697208No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA316840.16652779714429322No Hit
AACGTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA290890.15288874798416693No Hit
TACGTAGGGAGCGAGCGTTATCCGGAATTATTGGGTGTAAAGGGTGCGTA284810.1496931634410622No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA278800.1465343701673682No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA265230.13940211979731376No Hit
TACGGAGGATCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA259310.13629062958429072No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA248520.13061951819940584No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGAGCGTA245970.1292792648137287No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGCGCGTA244920.1287273957725675No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA241960.127171650666056No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA241670.12701922969278293No Hit
TACGTAGGTCCCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA240340.12632019557397875No Hit
TACGGAGGATACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA233030.12247813586837093No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA228230.11995530596591983No Hit
TACGGAGGATGCGAGCGTTATCCGGCTTTATTGGGTTTAAAGGGTGCGTA202050.1061953712063011No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA201020.10565401395640013No Hit
TACGGAGGGGGCAAGCGTTGTTCGGAGTGACTGGGCGTAAAGCGCACGTA197290.10369356488637042No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCATGTA195100.10254252374337712No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA193370.10163325379936869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAG6153100.0244.737189
AGGATGC7342700.0244.669666
AGGATCC1424200.0244.518316
TACGGAG13589550.0244.351871
GGAGGAT8815600.0244.087054
GATCCGA728600.0244.041668
GGATGCG6181150.0244.032877
GACACAA2243550.0244.017188
GGACACA2246700.0244.01317
ACACAAG2245600.0243.810799
ATCCGAG729100.0243.756689
TACGAGA306900.0243.283651
TATGTCG396550.0243.208285
TCAAACA7196900.0242.95746245
AGGACAC2263050.0242.88356
TACGTAT483900.0242.6711
TAGGTCC70850.0242.57945
GTCCAAG326850.0242.48899
ACGAGAT307500.0241.972352
GGAGGAC2311400.0241.650514
Produced by FastQC (version 0.11.2)