FastQCFastQC Report
Fri 4 Dec 2015
000000000-AKBTJ_l01n02.3320000003f34d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKBTJ_l01n02.3320000003f34d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16963708
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGACAAGGGA9364745.5204557871427635No Hit
AATCAGTCTCGT7214804.253079574347778No Hit
CCAGTGTATGCA6776023.99442150265732No Hit
GACGGAACCCAT6169433.6368404832245407No Hit
ATGTGCACGACT6088773.5892919166021957No Hit
CGGAGCTATGGT6004473.5395975926961256No Hit
GTCAATTGACCG5848503.4476542510635055No Hit
ATGAGACTCCAC5421003.1956456689775607No Hit
TGAGTCACTGGT5376643.169495725816549No Hit
GACTTGGTATTC5312093.131443903655969No Hit
GAATCTTCGAGC5032712.966751137192411No Hit
GACTTTCCCTCG4526512.6683493962522817No Hit
TCGAGGACTGCA4523322.666468911160225No Hit
ACACGTAAGCCT4510012.6586227492244032No Hit
GCGATATATCGC4497892.6514780848621067No Hit
AAGAGATGTCGA4468952.6344181354689673No Hit
GACAGGAGATAG4288492.5280380916719385No Hit
CCTGAACTAGTT4251782.506397775769307No Hit
TAGGAACTGGCC4251202.506055869388933No Hit
AATCCGTACAGC4094862.413894415065385No Hit
TCCCAGAACAAC4036542.3795151390250293No Hit
CCTCGTTCGACT3612812.1297289484115147No Hit
GAACTAGTCACC3419612.0158387541214453No Hit
TACACGATCTAC3401322.005056913264482No Hit
GGCCACGTAGTA3281001.934129024149673No Hit
CTAGCGAACATC3196311.8842047976774892No Hit
CAAGCATGCCTA2958601.7440762361625184No Hit
TCTAGCGTAGTG2690381.585962220052361No Hit
TAGGATTGCTCG2472211.4573523665934358No Hit
ACGCGCAGATAC2418741.4258321352855166No Hit
AGGCATCTTACG2402421.4162115971343057No Hit
ATTCCTGTGAGT1803051.062886722643422No Hit
CAGCTAGAACGC1769351.043020782956179No Hit
TCCAAAGTGTTC515430.3038427683381487No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACAGA550.0091598866.0000591
ATGTGCC1950.06.0000591
GGCCCCG751.7511669E-46.0000591
GGCTTGG909.151239E-66.0000591
CCTCAGT704.696882E-46.0000591
TCCCGGA751.7511669E-46.0000591
GTGAGAC953.4293844E-66.0000591
TCGAGTC550.0091598866.0000591
GGCGACC550.0091598866.0000591
CGGAGCA1001.2864348E-66.0000591
CGGCATC1601.0913936E-116.00005871
TCGGGGA650.00126184246.00005871
GCCGGAA2150.06.00005871
CGGGGCT852.444675E-56.00005871
TCCAGGA1303.6452548E-96.00005871
GACGGAC1850.06.00005871
TCGAGGC1850.06.00005871
TGCGTCA3850.06.00005871
ACGCGCC852.444675E-56.00005871
GTGCACA1405.18412E-105.99998863
Produced by FastQC (version 0.11.2)