FastQCFastQC Report
Mon 16 Nov 2015
000000000-AK0KC_l01n03.3330000003bea8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AK0KC_l01n03.3330000003bea8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14814934
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC13703009.249450588170017No Hit
GGATACCTTGTTACGACTTAGTCCCAATCAGTGGTTTTACCTTAGAGCCC13196488.907552338741436No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA8151685.502339733676843No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAA6536614.412176254042036No Hit
GGATACCTTGTGACGACTTAACCTACCTTGTCTTACAAAGGTTCGACCCT5370003.624720839120849No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC4316362.913519560735134No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT3751582.5322961276776526No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT3364842.271248727803985No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC3059712.065287634760978No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATAA2496831.6853466913858677No Hit
AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT2324201.5688223788239624No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGTTCAAGTTTGGGCAT2212771.4936077339257805No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTTACAAGA1946901.3141469276879667No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATTCA1890591.276137983469923No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATGA1393530.9406251826704053No Hit
GGATACCTTGTTACGACTTCTCCTTCCTCTAAACGATAAGGTTTAGTCGA1364630.9211178396069805No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA1264760.8537061319341686No Hit
GGATACCTTGTTACGACTTAGCCCCCCTCACCTATCTCTGGTTCGACAGC1219600.8232233771679307No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT1094950.7390853040587289No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAG989980.6682311240806068No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG988230.6670498835836866No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTCGGTCAG894650.6038838917540909No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC888640.599827174390382No Hit
GAAGACCTTGTTACGACTTCTCCTTCCTACAAACAAAAAGGTTCAGTAGG842990.5690136722850064No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC808740.5458951082738539No Hit
TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG752210.507737665250483No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC651200.439556463768249No Hit
GGATACCTTGTTACGACTTCTCCTTCCTCTAAACGATAAGGTTCAGTCGA634860.4285270524998626No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGTAAGGTTTGAACAA603330.40724447371820893No Hit
TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAATC558680.3771059661825021No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA481770.32519213382928336No Hit
GGATACCTTGTTACGACTTAGCCCCCCTCACCAACCTCTAGTTCGACAGC434030.29296789307330023No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT381050.25720668077225317No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA337230.2276284187293713No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGACTAA335640.2265551773635981No Hit
CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG333360.22501618974475351No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA328660.22184371526731064No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGAAAATTCGATTTGAGCAT323450.21832699355933682No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCTTACCGTGGCCGGC313780.21179979607064062No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA302250.20401710868236064No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC300800.20303836655634105No Hit
GGATACCTTGTTACGACTTCTCCTTCCTCTAAATGGTAAGGTTTGAGCAA291830.19698366526641292No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATAACGAGGTTAAAAAAA278400.18791848819576246No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAAGC269290.18176928766608072No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC258990.17481684359849325No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACGAA256890.173399355002189No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAA254310.1716578690124438No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCCA237490.1603044603506165No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC227000.15322376731479195No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGGCAT225270.15205602670926513No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTCACAAGA225120.1519547775238148No Hit
GGATACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA220810.14904555092854277No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG215560.14550182943778217No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC214130.14453658720315596No Hit
AGATACCTTGTGACGACTTAACCTACCTTGTCATAAAAGGATTTTTCGAA207650.14016262239170285No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAACGTGGTAAGC207230.139879124672442No Hit
GGATACCGTGTTACGACTTCTTCATCCTCTAGATGATAAGGCTCAGGGGA205900.13898138189478265No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAACGGTAAGGTTTAGCCTA204670.13815113857409017No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA194230.1311041952667491No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTTGGGCAT188730.1273917251335713No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGAACAA183340.12375350440305707No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACCAGTTTTACCTTCGGCGGC181140.12226851634978596No Hit
GGCTACCTTGTTACGACTTCACCCAAATTACCAACCACACCGTCGGCGCC179520.12117502514692269No Hit
GGATACCTTGTGACGACTTAACCTACCTTGTCTTACAAAGGTTCGTCTCT177820.12002753437848593No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA177570.11985878573606876No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA176640.11923104078627687No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGTTAAAAAGATTTGAACAA174580.11784055197275939No Hit
GGATACCTTGTTACGACTTAGTCCCAATCAGTGGTTTTACCTTAGAACCC161500.10901162300149296No Hit
AGATACCTTGTTACGACTTAACCCCGCTTGTAGACCCCAAGTTCGAATTC159660.10776963299330257No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC157510.10631839466851489No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAAAAAAA156630.10572439944720644No Hit
AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG155830.1051844037914715No Hit
GGATACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATTCA155600.1050291550404477No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCCAA153110.10334841856197267No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAAGCAA153010.1032809191050058No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGGTAAGGTTCAATCGG148980.10056069098924099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG12719250.094.970869
CTTGTTA12451950.094.965677
CCTTGTT12473750.094.949746
TTGTTAC12455250.094.906198
GATACCT3355450.094.8456952
GTTACCT3517050.094.7920152
TTGTGAC710700.094.6992348
CTTGTGA711350.094.6327447
CATGTTA249100.094.599567
ATGTTAC249100.094.580498
CCTTGTG714100.094.534386
AAACCTT4706400.094.53173
AACCTTG4755500.094.471624
AGATACC703500.094.196511
GGTTACC3477450.094.076621
GGATACC2913300.094.008691
GAAACCT4711450.093.900082
ATACCTT3410350.093.6991043
GGAAACC4692500.093.69421
CCATGTT252350.093.4753346
Produced by FastQC (version 0.11.2)