FastQCFastQC Report
Mon 16 Nov 2015
000000000-AK0KC_l01n02.3320000003beab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AK0KC_l01n02.3320000003beab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14814934
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGTCGCGAT9581496.467453719334829No Hit
ATTTCGACATGC8273815.58477682047048No Hit
CTAACCTCCGCT7639455.1565872652554505No Hit
GCACACACGTTA7498415.06138603114938No Hit
CACGCCATAATG7274784.910436995534371No Hit
GGAGACAAGGGA7189804.8530759570039255No Hit
TGCCCATTTAAG6636494.479594711660545No Hit
GCGGATGTGACT6575884.438683290792925No Hit
GAGTGAGTACAA6554784.424440905372917No Hit
GGATGGTGTTGC6486854.378588524255322No Hit
TGAGTCACTGGT6447674.352142237015704No Hit
AGTTCAGACGCT6268854.2314397080675485No Hit
GACTTGGTATTC6210304.1919187760134475No Hit
ACCGTGCATAGG6018454.06242106782251No Hit
TTCAGACTACCA5808853.920942206019953No Hit
TAATGACCACGC5682173.835433893934323No Hit
CAGGGTGATACC5531463.733705462339555No Hit
TGAGAACCTCCA5365033.621366116109596No Hit
TGCCTACAAGCA4767973.21835385834321No Hit
ATCGAATGCGGC3906992.6371970337498634No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGG1451.9645086E-106.00000054
GTTCTGA550.00916062956.00000052
CAACGGT550.00916062956.00000056
CCAGGGA2450.06.00000056
ATGTGTC550.00916062956.00000055
GTCAGTG550.00916062956.00000054
CAGGGTT1451.9645086E-106.00000051
GTTACTG550.00916062956.00000054
GTTCGGA550.00916062956.00000052
AGTTGGT1101.8152969E-76.00000052
GAGCGAG2450.06.00000051
GTCACGC550.00916062956.00000055
CGCAGTC1101.8152969E-76.00000051
GCAGGCG550.00916062956.00000052
GGTGAAC550.00916062956.00000054
ACATCCG1101.8152969E-76.00000054
TCAAGGG550.00916062956.00000055
AGTACGA550.00916062956.00000056
GGCTCGT550.00916062956.00000051
CTAATGC1451.9645086E-106.00000053
Produced by FastQC (version 0.11.2)