FastQCFastQC Report
Mon 16 Nov 2015
000000000-AK0KC_l01n01.3310000003beae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AK0KC_l01n01.3310000003beae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14814934
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTCATGAAAGGTGGGAGTACCCGAAGCGCCCTTAGCTAATTAAGCGGG14023879.466036095739609No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG11592597.824935298395524No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGTCCAGAGAG7296444.925057377913395No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCTTTGGGGA6251974.220045799731541No Hit
AAACCAACCGAGTTTTGCATTGGTGAGGCCCGTAAGGGTCGAACCTTTGT5789443.907840561422683No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG4755393.2098624266567772No Hit
ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC3578052.4151643200030453No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCTTAACCTTCGGGAG3160182.1331043391755915No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC2423741.6360113382887835No Hit
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACTGGAGATATGTT2053301.385966349900715No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC1582001.0678414092158628No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTTTGGCA1429980.965228734734829No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATCTGAGCA1364740.9211920890096439No Hit
AAGTCACCCAAGCTTGTTTTTGGTGAGGCTGCGCTTCTTGGCGCTGTCGA1276090.8613538204085148No Hit
GCTACTACCGATTGAATGTTTTAGTGAGGTCTTCAGACTGGCCGGCGCAG1267840.8557851152087481No Hit
GCTCCTACCGATTGAACGGTCCGGTGAAGTCTTCGGAGCGCGGTTCACGC1245400.8406382370653828No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGAATAA1224450.826497100830824No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTAGGGGG930540.6281094468595001No Hit
GCTACTACTGATGAATTGTTTAGTGAGGTCTCTGGACGTGTACAAGATGA891400.6016901594026676No Hit
GCTCCTACCGATGGAACGCAGAGGCAAATTAGGTGGAGACTCGCTCCGGT828470.5592127511334172No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTCAGCC819830.5533807980514797No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACCGATGTGCGGAG689440.4653682561123796No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGCGAATATGAC687450.46402501691873893No Hit
GCTACTACCGATTGAATGTTTTAGTGAGGTCTTCGGACTGGCCGGCGCAG665900.44947888394237867No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGTTAGTCTAACCCTTCGGGG631930.4265493184107334No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCAAAG624910.42181085653165923No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCGGTGGGGA618790.41767988976528686No Hit
GCTCCTACCGATTGAACGGTCCGGTGAAGTCTTCGGAGAGGGGCTCAAGC607530.41007945091081743No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTTCGGGG600260.4051722403893261No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTGGGTAGGCTAACCGCAAGGAG588420.39718030468444876No Hit
ACACCATGGGAGTGGAGGATACCAGAAGTAGTTAGGCTAACCGTAAGGGG572590.38649514064659346No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTCTCGTA541730.3656648082266178No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG471960.31857043710083355No Hit
AAGTCACCCAAGCTAGTTTTTAGTGAGGCGGCGCTTCTTGGCGCTGTCGA448550.30276881422488955No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG425920.28749368711328716No Hit
ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG346840.2341151165438874No Hit
GCTACTACCGATTGAACGGCTTAGTGAGGCTTTCGGATTGGCCCGGGGAG344190.2323263809342654No Hit
GCTACTACCGATTGGAAGATTCGATGAGAACTTTGGATTGGGCGCGAAGT320890.21659900746098495No Hit
ACACCATGGGAGTTGAATTCACCCGAAGCCGGAATACTAAACTAGTTACC314180.2120697938985081No Hit
GCTCCTACCGATTGAATGGTCCGGTGAGAACTTTGGATGGCGCAGTCTTT309050.2086070717561077No Hit
ACACCATGGGAGTTGGTTTTACCCGAAGACGGTACGCTAACCGCAAGGAG299760.20233637220388562No Hit
GCTACTACCGATTGAATGGCTTAGTGAGTTTCAGGGATTGTTGTGTTTGG296490.200129139961069No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTCAGCAATGA272170.18371327202672655No Hit
GCACCTACCGATTGAATGACTCGGTGAAAAATTGGGACTGTTGACTCGTT267050.18025729983002287No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTCGGGAG265440.1791705585728563No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATTCTGGCA260650.1759373345841433No Hit
ACACCATGGGAGCGGGTTCTGCCAGAAGTGGGTAGCCTAACCGCAAGGAG259500.17516109082902429No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA259040.1748505933269767No Hit
ACACCATGGGAGTCTATTGCACCAGAAGTAGGTAGCCTAACGAAAGAGGG256280.1729876083146911No Hit
GCTCCTACCGATTGAACGGTCCGGTGAAGTCTTCGGAGCGCGGCTCACGC244530.16505642212108404No Hit
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACCGGAAATATGTT234190.1580769782707098No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAATGTTCGGATCGCGGCGACGTG228140.15399326112421424No Hit
GCTACTACCGATTGAATGGCTTAGTGAGTTTCAGGGATTGTTGTATTTGG228090.1539595113957308No Hit
AAACCAACCGAGTTATGAAGGGATGAAGCGATTTTTCGAAAAATCCTTTT224300.1514012819766865No Hit
GCTCCTACCGATCGAACGGTCCGGCGAAGCCCTCGGAGCTTGGCCTGCTG222740.15034829044800335No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATTTTGGCA221130.14926154919083676No Hit
ACACCATGGGAGCGGGTTCCGCCAGAAGTAGGTAGCCTAACCGCAAGGGG216220.14594732585376352No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTA214190.14457708687733606No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGAG210530.14210660675234868No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGACGCAAGTTT200780.13552540969807897No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCCTTACCGC198790.1341821705044383No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCAGACTGGCCGGTGCAG195260.13179943967350782No Hit
AAACCAACCGAGTTTTGCATTGGTGAGACTCGTCAGAGACGAACCTTTGT190460.12855946573909813No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACTGGCCGGTGCAG190430.12853921590200806No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTTCAGGGATTGTTGCGCTTGG182370.12309875967047845No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTTCAGAGATTGTTGCGCTTGG178990.12081727802499828No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAG175560.1185020466510347No Hit
GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGAGGCAGCA174760.11796205099529974No Hit
ACACCCGGAAAATTGATTGCACCAGAAGTCGTTGGGCTAACTTCTATTTA173690.11723980680575426No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTTCAGAGATTGATGTACATAG168780.113925583468681No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACTGCTGTCTTGAG164090.11075985893693485No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATGTTG163920.11064510986009118No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGACTGGCTCAGAGAG162100.10941661974329417No Hit
AAACCATGCGAGTAGGCTCCAAGTGAGGGTCCGATTCCTGTCGAATTCGA161380.1089306236531327No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTTCAGAGATTGCTGCGCTTGG154270.10413141226278835No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG149880.10116818610194281No Hit
ACACCATGGGAGTTGGATTGACCCGAAGGCGCTGAGCTAACCCGCAAGGG149180.10069568990317473No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCGGTAGCAATA148680.10035819261834038No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTTTCATAT148340.10012869446465304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACC3492100.094.98643
ACTACCG3492600.094.9714364
CCATGGG4169900.094.867864
CTACTAC3565100.094.838782
ACCATGG4153900.094.836483
CCGAGTT760250.094.825058
CATGGGA4191600.094.813025
ACCGAGT715000.094.807357
GCTACTA3572000.094.771281
CACCATG4187450.094.740242
CTACCGA5249150.094.728525
ACACCAT4155000.094.698191
ACCGATT5113100.094.6432657
TACCGAT5380350.094.638046
TCCTACC1268150.094.636683
CCGATTG4995150.094.5226448
AACCGAG724900.094.482346
CGATTGA4622700.094.482129
GGGAGTG2751600.094.48048
CGAGTTT688700.094.4689259
Produced by FastQC (version 0.11.2)