##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJTU1_l01n02_plasmids_flu.3520000003bb9f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 462812 Sequences flagged as poor quality 0 Sequence length 301 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.833807247867384 34.0 34.0 34.0 32.0 34.0 2 33.37100161620701 34.0 34.0 34.0 34.0 34.0 3 33.49594867894523 34.0 34.0 34.0 34.0 34.0 4 33.56594038183971 34.0 34.0 34.0 34.0 34.0 5 33.462621971772556 34.0 34.0 34.0 34.0 34.0 6 37.11954746203642 38.0 38.0 38.0 36.0 38.0 7 35.96881887245793 38.0 38.0 38.0 34.0 38.0 8 36.46897876459556 38.0 37.0 38.0 34.0 38.0 9 36.673487290735764 38.0 38.0 38.0 35.0 38.0 10-14 36.93742469944599 38.0 38.0 38.0 36.2 38.0 15-19 36.95387241471699 38.0 38.0 38.0 36.0 38.0 20-24 36.92406765598126 38.0 38.0 38.0 35.6 38.0 25-29 36.76953190496357 38.0 38.0 38.0 35.4 38.0 30-34 36.42171378443083 38.0 37.4 38.0 33.4 38.0 35-39 36.739546943467325 38.0 38.0 38.0 35.0 38.0 40-44 36.160605602274785 38.0 37.2 38.0 32.6 38.0 45-49 36.471516296033805 38.0 37.4 38.0 34.0 38.0 50-54 36.34464231696671 38.0 37.0 38.0 34.0 38.0 55-59 35.883423074596166 38.0 37.0 38.0 31.8 38.0 60-64 35.92027302662852 38.0 37.0 38.0 31.8 38.0 65-69 35.73280986664132 38.0 36.6 38.0 30.8 38.0 70-74 35.555774266872945 38.0 36.8 38.0 29.8 38.0 75-79 35.47143159641496 38.0 36.4 38.0 29.4 38.0 80-84 34.8329857479927 38.0 35.6 38.0 26.2 38.0 85-89 34.873283752365964 38.0 35.8 38.0 26.0 38.0 90-94 34.71846970260062 38.0 35.6 38.0 25.0 38.0 95-99 33.62548810316068 37.6 34.0 38.0 21.6 38.0 100-104 33.90031719142978 37.6 34.0 38.0 23.0 38.0 105-109 33.68870686153341 37.4 33.8 38.0 22.4 38.0 110-114 33.25703655047838 37.0 33.4 38.0 21.4 38.0 115-119 33.26956820480022 37.0 33.4 38.0 21.6 38.0 120-124 32.43437119175821 36.8 30.4 38.0 18.2 38.0 125-129 31.87647554514576 36.0 28.4 38.0 16.2 38.0 130-134 31.661169978306525 36.0 27.8 38.0 15.2 38.0 135-139 30.958211973760406 35.6 26.4 38.0 13.0 38.0 140-144 29.057267313725657 34.6 21.8 37.4 9.8 38.0 145-149 28.46874756920737 34.0 21.4 37.0 10.0 38.0 150-154 27.534113635774354 31.8 20.0 37.0 9.2 38.0 155-159 27.070612689385758 30.4 19.4 37.0 9.0 37.0 160-164 26.569999913571813 29.2 18.8 37.0 9.2 37.2 165-169 25.301897530746825 27.2 16.8 35.4 8.2 37.0 170-174 24.40864281825018 25.4 15.6 35.0 8.2 37.0 175-179 23.225370128691566 23.6 13.0 35.0 7.8 37.0 180-184 21.91377492372713 21.8 10.6 33.4 7.4 36.2 185-189 21.652404432037194 21.2 10.8 32.8 8.0 36.0 190-194 20.58424932802088 20.0 9.8 30.2 7.0 35.4 195-199 19.72028642299681 19.2 9.0 28.6 7.0 35.0 200-204 19.050630925732264 18.4 8.6 27.6 7.0 35.0 205-209 18.506414267564367 17.4 8.0 26.4 7.0 34.6 210-214 17.51191628566243 15.4 8.0 25.2 7.0 33.2 215-219 17.042288013275368 15.0 8.0 24.2 7.0 32.2 220-224 16.219530608540833 13.2 8.0 23.0 7.0 31.0 225-229 15.588553451509469 12.4 8.0 21.6 7.0 29.4 230-234 15.165587322714192 12.0 8.0 20.8 7.0 28.4 235-239 14.466941220193082 11.8 8.0 19.4 7.0 27.2 240-244 13.897599889371927 11.2 7.8 18.8 7.0 25.4 245-249 13.258332541074992 11.0 7.4 17.4 7.0 24.4 250-254 12.809679524299284 9.4 7.0 16.4 7.0 23.4 255-259 12.31150964106376 9.0 7.0 15.2 7.0 22.2 260-264 11.881040249604592 9.0 7.0 14.0 7.0 21.0 265-269 11.540592724475598 8.0 7.0 13.4 7.0 20.6 270-274 11.245853175803566 8.0 7.0 13.0 7.0 19.4 275-279 10.931505665367364 8.0 7.0 13.0 7.0 19.0 280-284 10.739378408511447 8.0 7.0 13.0 7.0 18.8 285-289 10.616423083238983 8.0 7.0 13.0 7.0 18.0 290-294 10.510720119616604 8.0 7.0 12.6 7.0 18.0 295-299 10.301197030327648 8.0 7.0 12.0 7.0 17.0 300-301 10.116648876865767 8.0 7.0 12.0 7.0 15.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.29667423509724955 1101 2 0.15310776702902018 1101 3 0.1822313220921572 1101 4 0.1368975407623978 1101 5 0.10767698873427634 1101 6 0.21766158564120985 1101 7 1.3130767622510504 1101 8 0.5954186385644746 1101 9 0.3594074638735023 1101 10-14 0.30680369423242837 1101 15-19 0.3333555019011669 1101 20-24 0.40071229546828135 1101 25-29 0.30623914005361286 1101 30-34 0.6914930964607109 1101 35-39 0.37578310001431703 1101 40-44 0.7502072716354817 1101 45-49 0.4244447119462791 1101 50-54 0.3863405561310884 1101 55-59 0.5305738103569979 1101 60-64 0.5182588205020195 1101 65-69 0.5542521788005104 1101 70-74 0.6104448873804671 1101 75-79 0.5780476146939861 1101 80-84 0.8449394037683575 1101 85-89 0.5724357266970088 1101 90-94 0.6913607249471951 1101 95-99 1.3031443748462763 1101 100-104 0.7645507536547171 1101 105-109 0.8971802228647547 1101 110-114 0.6571986754351116 1101 115-119 1.0328613368115285 1101 120-124 1.159606516587118 1101 125-129 0.8076446548998675 1101 130-134 0.9973369898489572 1101 135-139 1.0232026588666479 1101 140-144 1.3439201262909393 1101 145-149 1.4891901900657523 1101 150-154 1.2951771170467268 1101 155-159 0.9476008171734556 1101 160-164 1.0555704727590332 1101 165-169 0.5994510424388295 1101 170-174 0.7222354765603214 1101 175-179 0.6095066475213855 1101 180-184 0.5371197903067326 1101 185-189 0.44415625127324887 1101 190-194 0.7054500098126972 1101 195-199 0.8599362082676159 1101 200-204 1.0939080885316699 1101 205-209 0.7026226173438879 1101 210-214 0.5933174746151906 1101 215-219 0.9997853973005277 1101 220-224 0.7470979646772591 1101 225-229 0.42857034898628044 1101 230-234 0.2916643525501126 1101 235-239 0.31597045707436244 1101 240-244 0.08923595901104164 1101 245-249 0.4261544161787896 1101 250-254 0.44084888619325113 1101 255-259 -6.401008996164137E-4 1101 260-264 0.14455469923914954 1101 265-269 0.2286249420581612 1101 270-274 0.1372586981899051 1101 275-279 -0.09545324238425401 1101 280-284 0.031003764277807377 1101 285-289 -0.04756311880934838 1101 290-294 -0.1418591206735833 1101 295-299 -0.06542264630518346 1101 300-301 -0.20504202652523773 1102 1 0.07155021448747334 1102 2 0.10311628346574508 1102 3 0.1134797252602695 1102 4 0.01916312584160096 1102 5 0.0756205886320842 1102 6 0.005586342922761389 1102 7 1.1116497268226695 1102 8 0.32041757400988047 1102 9 0.307718015738601 1102 10-14 0.18084985331946513 1102 15-19 0.19081110997474582 1102 20-24 0.22378385482784324 1102 25-29 0.11503289324726751 1102 30-34 0.5201583579153208 1102 35-39 0.26986204738273045 1102 40-44 0.5213455998589538 1102 45-49 0.05803513521318848 1102 50-54 0.1771816607129395 1102 55-59 0.26769961941848663 1102 60-64 0.26280297822643917 1102 65-69 0.3618743896674914 1102 70-74 0.1897174772288679 1102 75-79 0.28246019347102447 1102 80-84 0.8412837233050681 1102 85-89 0.36593981458663905 1102 90-94 0.4888073907622186 1102 95-99 1.1231074348019945 1102 100-104 0.7988161045319089 1102 105-109 1.081350296106109 1102 110-114 1.0086922455253884 1102 115-119 0.7254179711157391 1102 120-124 1.1137167618604948 1102 125-129 0.9968825402686292 1102 130-134 0.8234175127581729 1102 135-139 0.8819501039356332 1102 140-144 1.7969876403430547 1102 145-149 1.8348558560511137 1102 150-154 1.4966025556432143 1102 155-159 0.9881677989482789 1102 160-164 1.5210687907627864 1102 165-169 1.4064488920385578 1102 170-174 1.4336437758279068 1102 175-179 0.8703289094869788 1102 180-184 0.8688978093835509 1102 185-189 1.0565173268992076 1102 190-194 1.5061531481196297 1102 195-199 1.028763388475415 1102 200-204 1.0695606604955614 1102 205-209 0.6155702625491344 1102 210-214 1.1393929941178307 1102 215-219 0.9628150132092337 1102 220-224 0.8219824605042056 1102 225-229 0.8932968649120205 1102 230-234 0.6913210336947238 1102 235-239 0.595576252509554 1102 240-244 0.443487790044939 1102 245-249 0.4289680339611124 1102 250-254 0.5293170985931841 1102 255-259 0.3942761186541226 1102 260-264 0.45120071354676483 1102 265-269 0.36675622293024546 1102 270-274 0.36761021411564165 1102 275-279 0.23018229670619128 1102 280-284 0.19509580566768925 1102 285-289 -0.03475013975978136 1102 290-294 -0.1383407677524442 1102 295-299 0.01861701899885304 1102 300-301 -0.06224001238795296 1103 1 0.10616590768388079 1103 2 0.14015094328603084 1103 3 0.13671865337323652 1103 4 0.04840200046718479 1103 5 0.04451099299664918 1103 6 0.17281195267885607 1103 7 1.1549624675158654 1103 8 0.5036947455973859 1103 9 0.33423973161365694 1103 10-14 0.20528502661856862 1103 15-19 0.20785940907616407 1103 20-24 0.3190996977889071 1103 25-29 0.28076593774877523 1103 30-34 0.693198045549039 1103 35-39 0.27366927887609904 1103 40-44 0.754427809957356 1103 45-49 0.323935595204631 1103 50-54 0.3017379829208977 1103 55-59 0.3481213673859642 1103 60-64 0.4482667137848466 1103 65-69 0.5126103759747167 1103 70-74 0.4660372782558255 1103 75-79 0.6007384348060683 1103 80-84 1.0726519304081847 1103 85-89 0.6476310854467187 1103 90-94 0.5855465328246865 1103 95-99 1.3260569962055015 1103 100-104 0.906339371540902 1103 105-109 0.9185015584082095 1103 110-114 0.98077985784888 1103 115-119 0.9422472394084238 1103 120-124 1.312142628356007 1103 125-129 1.3697048017149243 1103 130-134 1.1415598961556874 1103 135-139 1.329650681599304 1103 140-144 1.7355453532228147 1103 145-149 1.7376770477498695 1103 150-154 2.190542970705259 1103 155-159 1.1408031220120378 1103 160-164 0.9983133885377136 1103 165-169 1.3025183721412539 1103 170-174 2.262838523367485 1103 175-179 1.749074095623051 1103 180-184 1.3192438727126081 1103 185-189 0.9700825280117407 1103 190-194 0.9909918836780385 1103 195-199 1.2526420256170532 1103 200-204 1.2031748025580349 1103 205-209 1.064592780734845 1103 210-214 1.3215193847896316 1103 215-219 1.516365237856217 1103 220-224 1.0719254964477223 1103 225-229 0.4080762294819795 1103 230-234 0.9215625292017826 1103 235-239 0.9860193954278262 1103 240-244 0.5575539004428851 1103 245-249 0.8145173493226849 1103 250-254 0.4061279137408107 1103 255-259 0.6310553762493303 1103 260-264 0.38169654153136 1103 265-269 0.3825194877997369 1103 270-274 0.44424425348235275 1103 275-279 0.24728809473785596 1103 280-284 0.22542715996807416 1103 285-289 0.15430206392014867 1103 290-294 0.0565014444854679 1103 295-299 0.020250256054907823 1103 300-301 -0.09329879501525262 2116 1 0.11358868496141383 2116 2 0.14258996767627252 2116 3 0.09659807239214757 2116 4 0.12091240775424694 2116 5 0.1682630972913728 2116 6 0.15421194484892453 2116 7 1.2436286382867223 2116 8 0.34798737632466015 2116 9 0.5888835348735171 2116 10-14 0.305021940841641 2116 15-19 0.2814028456341262 2116 20-24 0.2823916629717971 2116 25-29 0.26671538247596516 2116 30-34 0.685370721689921 2116 35-39 0.1831667276226554 2116 40-44 0.5938251660497116 2116 45-49 0.33180989866676214 2116 50-54 0.3831050893688399 2116 55-59 0.5941592903655177 2116 60-64 0.5945165669735815 2116 65-69 0.6076756906728065 2116 70-74 0.6254547311783512 2116 75-79 0.8803085714384125 2116 80-84 1.1989704260168992 2116 85-89 0.8057017897492713 2116 90-94 0.9520769586425857 2116 95-99 1.5002250004858695 2116 100-104 0.9681355583631159 2116 105-109 0.9286606365639969 2116 110-114 1.095875970286727 2116 115-119 1.0068786452730407 2116 120-124 1.4393023198566084 2116 125-129 1.1941963127616546 2116 130-134 1.1136636688788109 2116 135-139 1.6092231672118018 2116 140-144 2.1018936025801125 2116 145-149 2.1218830272093427 2116 150-154 2.3266645434779676 2116 155-159 2.2225087425995476 2116 160-164 1.8529838135726209 2116 165-169 1.7932273726501968 2116 170-174 1.9719656162435477 2116 175-179 2.340501884058238 2116 180-184 2.094727305877594 2116 185-189 1.2616397713530354 2116 190-194 1.5694728765136503 2116 195-199 1.0244250320506474 2116 200-204 0.7650182299810702 2116 205-209 0.6808150986562573 2116 210-214 1.526051089496729 2116 215-219 1.6801048142567936 2116 220-224 1.1270098639848172 2116 225-229 0.7269220972343966 2116 230-234 0.9790710442548303 2116 235-239 0.619180470216742 2116 240-244 0.5138064941084703 2116 245-249 0.6516675144037922 2116 250-254 0.7513716204314864 2116 255-259 1.3703799139569863 2116 260-264 0.49773224602965627 2116 265-269 0.5245448306855884 2116 270-274 0.36771317516996227 2116 275-279 0.4762270082040363 2116 280-284 0.2119283540329846 2116 285-289 0.13230021083580823 2116 290-294 0.08780447597485264 2116 295-299 0.11443600818530086 2116 300-301 0.004369189690274666 1117 1 -0.03649157186041663 1117 2 0.052702327226832324 1117 3 0.13351621043388207 1117 4 0.048681268107372944 1117 5 0.13856807321432285 1117 6 0.1959454922004511 1117 7 1.0662610620427415 1117 8 0.22278673427008044 1117 9 0.26946138399069497 1117 10-14 0.15526271130713099 1117 15-19 0.24400316666140753 1117 20-24 0.2819101220408129 1117 25-29 0.2951262974037263 1117 30-34 0.5580834022677692 1117 35-39 0.24079112569649652 1117 40-44 0.6569070280079714 1117 45-49 0.25010845404397486 1117 50-54 0.2814999529322506 1117 55-59 0.4121368184554086 1117 60-64 0.3890591030891599 1117 65-69 0.4129726409135799 1117 70-74 0.3423086637626156 1117 75-79 0.5944337720804711 1117 80-84 1.0289864773812667 1117 85-89 0.6321865409531213 1117 90-94 0.48915561032798394 1117 95-99 1.214350201127921 1117 100-104 0.6474292983310121 1117 105-109 0.6182272497261181 1117 110-114 0.9549449911534964 1117 115-119 0.9587245521751271 1117 120-124 1.1332028013975375 1117 125-129 1.1299908552977698 1117 130-134 1.0818819674341817 1117 135-139 1.2479069553337965 1117 140-144 1.367222655549611 1117 145-149 1.999411117096983 1117 150-154 2.2101316381810214 1117 155-159 2.0279662064839776 1117 160-164 1.989259897039716 1117 165-169 1.5254906150257952 1117 170-174 1.53376336905254 1117 175-179 1.7937924137532626 1117 180-184 1.3419183171135494 1117 185-189 1.3086702689453311 1117 190-194 -0.5314902053483088 1117 195-199 0.9358215007504853 1117 200-204 1.278019835117508 1117 205-209 1.2328215192020018 1117 210-214 1.5727605598017043 1117 215-219 1.6618062588795866 1117 220-224 1.3766085798756684 1117 225-229 0.9115127874430691 1117 230-234 0.6593278660846877 1117 235-239 0.630416425272923 1117 240-244 0.7487984684262852 1117 245-249 0.9195647856718505 1117 250-254 0.66758349844112 1117 255-259 0.6764794324160555 1117 260-264 0.8121784739590314 1117 265-269 0.4237422624672895 1117 270-274 0.4108913453144236 1117 275-279 0.13754322008204767 1117 280-284 0.19860572160922807 1117 285-289 0.1591059893269815 1117 290-294 0.09904043103103177 1117 295-299 0.11042316709380806 1117 300-301 -0.09996467868853642 2117 1 0.16245804030705813 2117 2 0.1889235696101963 2117 3 0.13601898159005543 2117 4 0.07367535070270037 2117 5 0.03132335910581219 2117 6 0.21320620593645856 2117 7 1.1114118297532443 2117 8 0.3704793344747017 2117 9 0.2908849757453851 2117 10-14 0.15860866412569408 2117 15-19 0.2702076333642083 2117 20-24 0.24284464514973791 2117 25-29 0.2560994938240597 2117 30-34 0.6302406875306517 2117 35-39 0.31333346262510275 2117 40-44 0.761870404770093 2117 45-49 0.4051767919840472 2117 50-54 0.3789145321239431 2117 55-59 0.6311489546320246 2117 60-64 0.567661379770179 2117 65-69 0.5831666412472813 2117 70-74 0.5238623996174354 2117 75-79 0.5916725752514225 2117 80-84 1.0532885793210127 2117 85-89 0.5855902450544264 2117 90-94 0.45745759327292745 2117 95-99 1.4126377106830788 2117 100-104 0.8359716507806851 2117 105-109 0.7720491170908872 2117 110-114 0.988944005566097 2117 115-119 0.9045074151125192 2117 120-124 1.2374070689133845 2117 125-129 0.9855025782261322 2117 130-134 0.9456621478147902 2117 135-139 1.6854031235717528 2117 140-144 2.370686827119428 2117 145-149 2.2249417110491976 2117 150-154 2.2090503375678097 2117 155-159 2.066833401815238 2117 160-164 1.692305776003888 2117 165-169 1.6205007231170612 2117 170-174 1.2563062880209586 2117 175-179 2.12401916352659 2117 180-184 1.3171274310808272 2117 185-189 1.2194924583721267 2117 190-194 1.2003623756101938 2117 195-199 1.298354046476181 2117 200-204 1.765384009662192 2117 205-209 1.1966708976481684 2117 210-214 1.7467365254380738 2117 215-219 1.582411502670194 2117 220-224 1.2204139302442307 2117 225-229 0.9284681242953674 2117 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-0.5488479629172005 2110 130-134 -1.2625544912568394 2110 135-139 -1.5409305585414543 2110 140-144 -0.38220330187567697 2110 145-149 -0.6854415058237251 2110 150-154 -1.089482916120776 2110 155-159 -1.457544974901765 2110 160-164 -0.8772081905586795 2110 165-169 -1.429849058995746 2110 170-174 -1.5919363825905286 2110 175-179 -0.9505764322255139 2110 180-184 -1.4920152786848782 2110 185-189 -0.9325878598861657 2110 190-194 -1.1362726068773128 2110 195-199 -1.4631643085656911 2110 200-204 -0.8639772420967411 2110 205-209 -0.8528363338548068 2110 210-214 -0.8540774278949144 2110 215-219 -0.9526474224196413 2110 220-224 -0.8636074081350547 2110 225-229 -0.9671500780049715 2110 230-234 -0.6933490915562164 2110 235-239 -0.9783902635941395 2110 240-244 -0.6665224656939088 2110 245-249 -0.69786545211727 2110 250-254 -0.7793285438088393 2110 255-259 -0.7103190524203296 2110 260-264 -0.47002048310157285 2110 265-269 -0.5529244308185532 2110 270-274 -0.2645977155343555 2110 275-279 -0.18959982339505643 2110 280-284 -0.33010412668807554 2110 285-289 -0.29493387023023665 2110 290-294 -0.36663733317096714 2110 295-299 -0.26054010110239645 2110 300-301 -0.5012933749610529 2109 1 0.13291107168724636 2109 2 0.04629055640629787 2109 3 0.010225802237826542 2109 4 -0.010695280160788911 2109 5 -0.13042592878635872 2109 6 -0.24207450653056384 2109 7 0.7402442168850811 2109 8 0.27448252244067106 2109 9 -0.001953147556513102 2109 10-14 -0.037995040664867474 2109 15-19 -0.16618600724006427 2109 20-24 -0.31986862292878726 2109 25-29 -0.27668850576118587 2109 30-34 -4.287233689802797 2109 35-39 -0.2232917528031848 2109 40-44 0.008630220946990619 2109 45-49 -0.5085006257093028 2109 50-54 -0.3315841365072032 2109 55-59 -0.03925738226359243 2109 60-64 -0.23778879504610728 2109 65-69 -0.22843812416545006 2109 70-74 -0.3886305098013594 2109 75-79 -0.832667788911337 2109 80-84 -0.45594913491280664 2109 85-89 -0.6299971735944965 2109 90-94 -0.3658187601385876 2109 95-99 -0.2568249774418092 2109 100-104 -0.8067136141613744 2109 105-109 -0.5897294480397974 2109 110-114 -0.8286398225548339 2109 115-119 -0.5825298842248827 2109 120-124 -0.6362005171444167 2109 125-129 -1.2967992633257275 2109 130-134 -1.2113360948731788 2109 135-139 -1.1748404874503144 2109 140-144 -0.47990180124873305 2109 145-149 -0.921403412020247 2109 150-154 -0.7961741585349991 2109 155-159 -1.7278371919773114 2109 160-164 -1.4286784813809916 2109 165-169 -1.3757490794987852 2109 170-174 -1.326031675679335 2109 175-179 -1.121556686546839 2109 180-184 -1.1043445769649942 2109 185-189 -1.2821406287025852 2109 190-194 -1.042296594034326 2109 195-199 -1.689163413126991 2109 200-204 -1.6616135349805141 2109 205-209 -1.2107085929685475 2109 210-214 -1.1439193592529797 2109 215-219 -0.7658046528082103 2109 220-224 -0.886056000608626 2109 225-229 -1.0567345358670508 2109 230-234 -0.9367890267275687 2109 235-239 -0.9076447481101635 2109 240-244 -0.8228666210037066 2109 245-249 -0.6555863793008072 2109 250-254 -0.628035248580467 2109 255-259 -0.4346581084717247 2109 260-264 -0.4626264189396263 2109 265-269 -0.4565105093967876 2109 270-274 -0.4239082556289464 2109 275-279 -0.168332875264948 2109 280-284 -0.3047662798973594 2109 285-289 -0.7165249615914853 2109 290-294 -0.322427627563874 2109 295-299 -0.17072815717750167 2109 300-301 -0.3663994398148045 2108 1 0.11862764363727507 2108 2 0.08226608103659316 2108 3 -0.02532341239155045 2108 4 0.0038544213881550604 2108 5 0.04658465745799134 2108 6 -0.2573944263080108 2108 7 1.115710961640481 2108 8 0.24240644040663994 2108 9 -0.12074858345167883 2108 10-14 -0.22840115882627998 2108 15-19 -0.07095761472159978 2108 20-24 -0.11078345286446734 2108 25-29 -0.14943319987013126 2108 30-34 0.13674236601248424 2108 35-39 -0.23692945059297443 2108 40-44 -0.19451725439206058 2108 45-49 -0.23429026964510768 2108 50-54 -0.2631143919186485 2108 55-59 -0.03412477329406016 2108 60-64 -0.20901382339300056 2108 65-69 -0.4022956508183455 2108 70-74 -0.43236501264966876 2108 75-79 -0.4050954745451705 2108 80-84 -0.18158064916457306 2108 85-89 -0.45313913278177864 2108 90-94 -0.323882386565721 2108 95-99 -0.17842343564335295 2108 100-104 -0.8237623367636289 2108 105-109 -0.765056586484981 2108 110-114 -1.0849059326122656 2108 115-119 -0.8456346604632117 2108 120-124 -1.1085959911759424 2108 125-129 -0.8352252532536397 2108 130-134 -0.6696543495900009 2108 135-139 -1.048115685538047 2108 140-144 -0.5889410033816418 2108 145-149 -1.0031932330177185 2108 150-154 -0.7974409666239879 2108 155-159 -1.8629202577318793 2108 160-164 -1.0579823225975389 2108 165-169 -1.1080020188926198 2108 170-174 -1.5863283901639953 2108 175-179 -1.0651670419300245 2108 180-184 -1.7242550246245436 2108 185-189 -1.411162328971244 2108 190-194 -1.2422400705038505 2108 195-199 -0.9469425831845015 2108 200-204 -1.1314363030671934 2108 205-209 -0.9115172880581532 2108 210-214 -1.0116368641817637 2108 215-219 -0.7352492580458296 2108 220-224 -0.9765271548804115 2108 225-229 -0.8650609286102462 2108 230-234 -0.5891217800704602 2108 235-239 -0.6278675734013781 2108 240-244 -0.38142111683765023 2108 245-249 -0.7880309538931414 2108 250-254 -0.42113583662948706 2108 255-259 -0.4818296505766462 2108 260-264 -0.35454321357874186 2108 265-269 -0.46793507261631184 2108 270-274 -0.40527946199771847 2108 275-279 -0.21802114458935584 2108 280-284 -0.5698409352230005 2108 285-289 -0.8071185608124143 2108 290-294 -0.5750867191694482 2108 295-299 -0.4430504911226052 2108 300-301 2.581522639433908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 39.0 11 248.0 12 593.0 13 1324.0 14 2334.0 15 3264.0 16 4601.0 17 6771.0 18 9127.0 19 12141.0 20 16856.0 21 23534.0 22 30642.0 23 36129.0 24 38151.0 25 38678.0 26 40059.0 27 46409.0 28 52315.0 29 48479.0 30 33012.0 31 14443.0 32 3365.0 33 278.0 34 13.0 35 3.0 36 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.287036636906564 23.032462425347656 16.46975445753351 22.21074648021227 2 37.95947382522493 25.59894730473713 17.259924116055764 19.181654753982176 3 21.091069375902094 23.68650769642965 19.3259898187601 35.896433108908155 4 22.118268324935393 22.46354891402989 19.556104854671013 35.86207790636371 5 37.482174187359014 22.236242794050284 20.672108761224862 19.609474257365843 6 35.37591937979136 21.607045625437543 20.97525561134975 22.041779383421346 7 35.842199424388305 22.021468760533438 21.537903079436145 20.59842873564212 8 21.954270848638323 33.270528854048735 22.338876260771112 22.436324036541837 9 34.796850557029636 19.662411519148165 23.618661573165777 21.922076350656422 10-14 28.917530228256833 21.046861360552448 25.321210340267754 24.714398070922964 15-19 22.863581756739237 20.46113756773809 22.317830998331935 34.357449677190736 20-24 26.72700794274997 21.032254997709654 22.664451224255206 29.57628583528517 25-29 27.490384864696683 20.694925801405322 24.5308678253805 27.283821508517498 30-34 24.696766721692608 20.653613130169486 24.861844550270952 29.78777559786695 35-39 26.362237798501337 21.898222172285937 24.919146435269614 26.820393593943116 40-44 27.204437222889638 21.395858361494515 26.97099470195241 24.428709713663434 45-49 27.96401130480627 22.638133842683423 24.315488794586138 25.082366057924165 50-54 26.894592188620862 23.082201844377416 27.571584142157075 22.451621824844644 55-59 23.968263571385357 25.599811586562147 22.14609819970096 28.285826642351537 60-64 30.013396368287772 20.964192803989526 22.153098882483597 26.869311945239104 65-69 29.210997121941524 20.851792952645997 22.479667770066463 27.457542155346015 70-74 24.496728693292308 20.84487869804586 24.43959966465865 30.21879294400318 75-79 27.857704640329118 21.191412495786626 26.488293302680137 24.46258956120412 80-84 28.879761112503566 21.29776237435503 25.012359230097754 24.810117283043656 85-89 27.387405685245845 23.58975134611894 24.064544566692305 24.958298401942905 90-94 29.458224937987783 20.729756358953527 27.571540928065826 22.24047777499287 95-99 26.26215396316431 21.04655886191369 26.785519822303655 25.90576735261834 100-104 26.375029169511592 20.926855829148767 29.496944763748562 23.20117023759107 105-109 26.764993128959492 25.798293907677415 21.397759781509553 26.038953181853543 110-114 27.607451837895304 21.625886969222925 26.792736575542552 23.97392461733922 115-119 24.7875595274107 21.528223122995946 23.717016844852772 29.96720050474059 120-124 25.06775969508137 20.665108078442216 24.228801327536882 30.038330898939524 125-129 24.411424077163083 20.951963216165527 23.75003241056844 30.886580296102956 130-134 24.573087992532606 20.53036654192199 24.036412193287987 30.860133272257418 135-139 27.375997165155614 20.789823945792243 21.138561662186806 30.695617226865334 140-144 27.49907089703811 20.564721744466436 21.92739168388028 30.00881567461518 145-149 25.247443886502513 21.47264980164732 26.06470878023906 27.215197531611107 150-154 27.939724985523277 23.450731614564877 23.193910270260925 25.415633129650917 155-159 25.075149304685272 22.51618367717345 21.178793981141368 31.229873036999905 160-164 20.96242102624824 20.5950148224333 23.520824870573797 34.92173928074466 165-169 25.68096764993129 20.371554756575026 21.01527185984806 32.932205733645624 170-174 28.208257348556216 20.822104871956647 20.826858421994242 30.142779357492895 175-179 30.133056186961444 22.360137593666543 21.201827091778085 26.304979127593924 180-184 22.552008158820428 24.053136046602074 26.44702384553555 26.947831949041944 185-189 21.769530608540833 20.603873711139727 27.553218153375454 30.073377526943982 190-194 24.118821465303405 20.89029670795053 22.133782183694457 32.857099643051605 195-199 25.810437067318908 22.606717198344036 22.59306154550876 28.989784188828295 200-204 25.9480307338617 22.207159710638443 22.22072893529122 29.624080620208638 205-209 26.217124880080895 20.717224272490775 26.25221472217661 26.813436125251723 210-214 25.954080706636823 21.33687112693707 20.642118181896755 32.06692998452936 215-219 28.81584747154352 21.288687415192346 21.73435433826262 28.161110775001514 220-224 23.566675021390974 23.417716048849208 25.203884082521625 27.81172484723819 225-229 26.297157377077518 23.76157057293242 21.867410525224066 28.073861524766 230-234 22.969024139391372 26.532803816668537 20.39406929811673 30.104102745823358 235-239 27.42673050828414 22.6890832562682 21.241800126185144 28.642386109262507 240-244 23.424241376628093 21.945411959931896 26.87717690984676 27.753169753593255 245-249 23.480678979801734 24.229492752996897 22.105390525742635 30.184437741458737 250-254 24.23109167437318 24.246605533132243 22.169995592162692 29.352307200331886 255-259 23.845838050871627 25.155095373499393 20.606207272067277 30.392859303561703 260-264 23.46010907236632 23.954391848093827 22.35996473730154 30.22553434223832 265-269 23.192873132070908 25.170436375893452 21.867021598402808 29.769668893632833 270-274 23.246717889769496 24.33126193789271 21.70077698936069 30.721243182977105 275-279 22.83700509061995 25.210279768026755 21.51551818016819 30.437196961185105 280-284 22.548896744250367 24.85238066428701 22.42223624279405 30.176486348668575 285-289 21.45393810013569 25.124067655981264 20.455044380871716 32.96694986301133 290-294 20.564332817645177 25.904082003059557 19.80212267616224 33.72946250313302 295-299 21.375547738606603 26.285921713352288 19.351788631236875 32.98674191680423 300-301 16.76793168716455 25.38849468034537 24.938852060880013 32.90472157161007 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.25 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.25 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 1.0 27 0.75 28 1.25 29 1.5 30 3.5 31 5.25 32 8.75 33 31.0 34 95.75 35 269.5 36 638.25 37 1211.5 38 1904.25 39 2589.0 40 3544.25 41 4722.0 42 6861.75 43 9463.25 44 11109.25 45 11319.5 46 10558.0 47 8854.5 48 6831.5 49 4996.75 50 3496.25 51 2503.0 52 1937.75 53 1631.5 54 1410.25 55 1215.0 56 1080.25 57 1002.0 58 975.0 59 926.25 60 934.25 61 959.0 62 1004.75 63 1220.75 64 1742.75 65 2704.5 66 3718.5 67 4123.5 68 3923.0 69 3440.5 70 3245.5 71 3294.25 72 3245.75 73 3020.25 74 2673.75 75 2275.25 76 1942.5 77 1613.75 78 1351.0 79 1161.75 80 997.25 81 858.0 82 765.0 83 664.75 84 581.0 85 444.5 86 358.0 87 271.25 88 178.0 89 96.5 90 38.0 91 15.75 92 8.75 93 8.0 94 4.75 95 3.75 96 3.75 97 1.75 98 1.25 99 2.25 100 0.6666666666666666 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260-264 0.0 265-269 0.0 270-274 0.0 275-279 0.0 280-284 0.0 285-289 0.0 290-294 0.0 295-299 0.0 300-301 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 301 462812.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.648567381910391 #Duplication Level Percentage of deduplicated Percentage of total 1 62.161972021631726 5.997739756431422 2 4.915932929258351 0.9486342022580265 3 3.3686321852551826 0.9750742387292004 4 2.5818755743462396 0.9964560180315312 5 2.4187124827485285 1.1668555183633473 6 2.069206695556449 1.1978928137505922 7 1.590674741764363 1.0743412700031456 8 1.279281644490718 0.987458811784786 9 1.333136035521569 1.1576567581184467 >10 13.011481067271394 28.454116166821613 >50 4.129739601392597 27.551603177144795 >100 1.1275192168134636 14.813317801156808 >500 0.0023671607898942903 0.15553842772260784 >1k 0.004734321579788268 1.1330780322052962 >5k 0.002367160789894134 1.8431646280783593 >10k+ 0.002367160789894134 11.547072379399951 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGG 50557 10.923874056852458 No Hit GGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAG 8070 1.7436885819728096 No Hit GGCCGCGCTCCCCTCGCGCACACTGCGGAGCGCACAAAGCCCCGCGCCGC 2952 0.6378399868629162 No Hit GGCCGCCCGTGCCACACGCAGGGGCCGGCCCGTCTAGAATTGACCTGCAT 2009 0.43408554661504023 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 681 0.14714398070922965 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 2.1607045625437544E-4 0.0 0.0 6 4.3214091250875087E-4 0.0 0.0 7 4.3214091250875087E-4 0.0 0.0 8 8.642818250175017E-4 0.0 0.0 9 8.642818250175017E-4 0.0 0.0 10-14 8.642818250175017E-4 0.0 0.0 15-19 9.93924098770127E-4 0.0 0.0 20-24 0.0018149918325367537 0.0 0.0 25-29 0.002333560927547255 0.0 0.0 30-34 0.003543555482571757 0.0 0.0 35-39 0.004321409125087509 0.0 0.0 40-44 0.007432823695150514 0.0 0.0 45-49 0.012921013284011651 0.0 0.0 50-54 0.018236346507869285 0.0 0.0 55-59 0.02597166884177593 0.0 0.0 60-64 0.038158042574522696 0.0 0.0 65-69 0.05773402591116912 0.0 0.0 70-74 0.08297105520168016 0.0 0.0 75-79 0.11395555862855762 0.0 0.0 80-84 0.15263217029809079 0.0 0.0 85-89 0.20172337795908488 0.0 0.0 90-94 0.2660691598316379 0.0 0.0 95-99 0.3468795104707743 0.0 0.0 100-104 0.44488906942775897 0.0 0.0 105-109 0.5622585412651357 0.0 0.0 110-114 0.7049082564842744 0.0 0.0 115-119 0.8821724587953639 0.0 0.0 120-124 1.0729194575767267 0.0 0.0 125-129 1.3094733930840168 0.0 0.0 130-134 1.5730793497143547 0.0 0.0 135-139 1.8491741787161957 0.0 0.0 140-144 2.166365608497619 0.0 0.0 145-149 2.53087646819875 0.0 0.0 150-154 2.929353603623069 0.0 0.0 155-159 3.3945532959387394 0.0 0.0 160-164 3.8629076169157237 0.0 0.0 165-169 4.424517946812097 0.0 0.0 170-174 4.968972282481872 0.0 0.0 175-179 5.57431527272413 0.0 0.0 180-184 6.189424647589085 0.0 0.0 185-189 6.844636699134854 0.0 0.0 190-194 7.547427465147836 0.0 0.0 195-199 8.243865759746939 0.0 0.0 200-204 8.928852320164559 0.0 0.0 205-209 9.556191282853513 0.0 0.0 210-214 10.198352678841518 0.0 0.0 215-219 10.833383749773127 0.0 0.0 220-224 11.436738891817843 0.0 0.0 225-229 11.969741493306136 0.0 0.0 230-234 12.467049255421207 0.0 0.0 235-239 12.903122650233787 0.0 0.0 240-244 13.272948843158778 0.0 0.0 245-249 13.564816815467186 0.0 0.0 250-254 13.815501758813514 0.0 0.0 255-259 14.023577608186477 0.0 0.0 260-264 14.192242206338642 0.0 0.0 265-269 14.319637347346225 0.0 0.0 270-274 14.41635048356568 0.0 0.0 275-279 14.484153392738303 0.0 0.0 280-284 14.531429608566762 0.0 1.2964227375262524E-4 285-289 14.568766583407518 0.0 3.457127300070007E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGCGT 6570 0.0 270.97794 8 GGGCGTC 6910 0.0 259.7793 9 GAGGGCG 7150 0.0 243.63287 7 GGAGGGC 8095 0.0 216.10254 6 GCCGGGA 9330 0.0 187.02306 2 CGGGAGG 9730 0.0 179.48613 4 CCGGGAG 10000 0.0 174.1975 3 GGGAGGG 10455 0.0 167.46294 5 GGCCGGG 12155 0.0 166.85521 1 CGTGCCA 490 0.0 162.55103 8 GGCGGTG 1925 0.0 157.84415 8 GGGCGGT 2435 0.0 119.33264 7 TAGGGCG 150 0.0 118.00001 7 GCCGGTA 190 0.0 100.92105 2 CCGCGCT 965 0.0 93.23834 3 CGGGGGC 3235 0.0 89.82226 4 CGCGCTC 1020 0.0 89.65686 4 GCGGTGC 3515 0.0 87.702705 9 CCCGTGC 885 0.0 86.66667 6 GCCCGTG 905 0.0 84.75137 5 >>END_MODULE