FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_plasmids_flu.3520000003bb9f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_plasmids_flu.3520000003bb9f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462812
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGG5055710.923874056852458No Hit
GGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAG80701.7436885819728096No Hit
GGCCGCGCTCCCCTCGCGCACACTGCGGAGCGCACAAAGCCCCGCGCCGC29520.6378399868629162No Hit
GGCCGCCCGTGCCACACGCAGGGGCCGGCCCGTCTAGAATTGACCTGCAT20090.43408554661504023No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT6810.14714398070922965Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGT65700.0270.977948
GGGCGTC69100.0259.77939
GAGGGCG71500.0243.632877
GGAGGGC80950.0216.102546
GCCGGGA93300.0187.023062
CGGGAGG97300.0179.486134
CCGGGAG100000.0174.19753
GGGAGGG104550.0167.462945
GGCCGGG121550.0166.855211
CGTGCCA4900.0162.551038
GGCGGTG19250.0157.844158
GGGCGGT24350.0119.332647
TAGGGCG1500.0118.000017
GCCGGTA1900.0100.921052
CCGCGCT9650.093.238343
CGGGGGC32350.089.822264
CGCGCTC10200.089.656864
GCGGTGC35150.087.7027059
CCCGTGC8850.086.666676
GCCCGTG9050.084.751375
Produced by FastQC (version 0.11.2)