##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_8561_flu.3520000003bac6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346804
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.919729876241334	34.0	34.0	34.0	33.0	34.0
2	33.20235060725943	34.0	34.0	34.0	34.0	34.0
3	33.21418726427608	34.0	34.0	34.0	34.0	34.0
4	33.30819713728792	34.0	34.0	34.0	34.0	34.0
5	33.20115396592888	34.0	34.0	34.0	34.0	34.0
6	36.62575691168499	38.0	38.0	38.0	34.0	38.0
7	35.94429706693118	38.0	37.0	38.0	33.0	38.0
8	36.5756767511332	38.0	38.0	38.0	34.0	38.0
9	36.62335497860463	38.0	38.0	38.0	35.0	38.0
10-14	36.62234749310851	38.0	38.0	38.0	34.8	38.0
15-19	36.52241438968409	38.0	38.0	38.0	34.8	38.0
20-24	36.61193757857464	38.0	38.0	38.0	34.8	38.0
25-29	36.53420375774213	38.0	38.0	38.0	34.0	38.0
30-34	36.17714789910151	38.0	37.4	38.0	32.6	38.0
35-39	36.41373282891778	38.0	37.6	38.0	34.0	38.0
40-44	35.914447930243014	38.0	37.0	38.0	32.0	38.0
45-49	36.171413247828745	38.0	37.0	38.0	34.0	38.0
50-54	36.13097945813774	38.0	37.0	38.0	33.4	38.0
55-59	35.73493096965433	38.0	37.0	38.0	30.8	38.0
60-64	35.89121059734028	38.0	37.0	38.0	31.8	38.0
65-69	35.74143031798941	38.0	37.0	38.0	31.4	38.0
70-74	35.60881420052825	38.0	37.0	38.0	30.8	38.0
75-79	35.64430283387735	38.0	37.0	38.0	30.6	38.0
80-84	34.902939412463525	38.0	36.0	38.0	26.4	38.0
85-89	35.03805319431149	38.0	36.0	38.0	27.0	38.0
90-94	35.00763486003622	38.0	36.0	38.0	26.6	38.0
95-99	34.04307908789979	37.6	34.6	38.0	23.0	38.0
100-104	34.33307458968178	38.0	35.0	38.0	24.0	38.0
105-109	34.114304909977974	37.8	34.6	38.0	23.6	38.0
110-114	33.55574560847049	37.0	34.0	38.0	23.0	38.0
115-119	33.57189363444481	37.0	34.0	38.0	23.0	38.0
120-124	33.117059203469395	37.0	32.6	38.0	21.2	38.0
125-129	32.58421586832908	37.0	30.8	38.0	19.2	38.0
130-134	32.299172443224414	36.6	30.0	38.0	18.6	38.0
135-139	31.593229605194864	36.0	28.0	38.0	13.8	38.0
140-144	30.33404747350088	35.0	25.2	37.6	10.8	38.0
145-149	30.16952745643072	35.0	24.2	37.4	10.6	38.0
150-154	29.098382371598944	34.0	22.8	37.0	10.0	38.0
155-159	28.81910358588713	33.6	22.6	37.0	10.0	37.0
160-164	28.027923553361553	32.2	21.4	37.0	10.0	37.2
165-169	27.066921373455898	30.2	20.0	35.8	9.4	37.0
170-174	26.415846414689565	29.0	20.0	35.4	9.0	37.0
175-179	25.570999181093647	27.2	17.8	35.0	9.0	37.0
180-184	24.736449406581237	25.6	16.0	35.0	8.6	36.6
185-189	24.053282545760716	25.0	15.0	35.0	8.2	36.2
190-194	23.186772932261448	24.0	14.6	33.8	8.0	36.0
195-199	22.35923922446108	23.0	11.8	32.2	8.0	36.0
200-204	21.528456419187783	22.4	9.6	30.4	8.0	35.0
205-209	20.75193250366201	20.8	9.0	28.8	8.0	35.0
210-214	19.632533073436292	19.4	9.0	27.2	8.0	34.4
215-219	19.208453189697927	18.8	9.0	26.2	8.0	34.0
220-224	18.56568436350215	17.8	9.0	25.8	8.0	33.0
225-229	17.946294737084923	16.6	9.0	25.2	8.0	32.2
230-234	17.102081867567854	15.2	9.0	24.0	8.0	30.8
235-239	16.208472797314908	14.4	8.8	22.6	8.0	29.0
240-244	15.457359200009227	13.2	8.0	21.2	7.0	28.0
245-249	14.935082063644018	12.8	8.0	20.4	7.0	26.8
250-254	14.286777545818387	12.0	8.0	19.4	7.0	25.6
255-259	13.700318912123276	11.2	8.0	18.2	7.0	24.6
260-264	13.164825088522624	10.2	8.0	17.2	7.0	23.6
265-269	12.790923980115569	9.8	8.0	16.0	7.0	22.6
270-274	12.38973310573119	9.0	8.0	15.6	7.0	21.8
275-279	11.928468529774744	9.0	7.0	14.2	7.0	21.0
280-284	11.803902492474135	8.4	7.2	14.0	7.0	20.8
285-289	11.418927117334286	8.0	7.8	13.2	7.0	19.6
290-294	11.20830382579209	8.0	7.4	13.0	7.0	19.0
295-299	10.903563396039262	8.0	7.0	13.0	7.0	19.0
300-301	10.587295705931881	8.0	7.0	12.5	7.0	17.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.3628991556306289
1101	2	0.24252788049829377
1101	3	0.3047387800139916
1101	4	0.1586482103306892
1101	5	0.2068354534607053
1101	6	0.121233085137888
1101	7	0.9720097660070479
1101	8	0.4704769095625849
1101	9	0.36003730510134346
1101	10-14	0.1687540563330998
1101	15-19	0.33893634284856944
1101	20-24	0.17572090265142748
1101	25-29	0.23057349302615648
1101	30-34	0.6201627726397021
1101	35-39	0.3377765242235
1101	40-44	0.7365764189677293
1101	45-49	0.2565543505446328
1101	50-54	0.37287003112479766
1101	55-59	0.5099728645114894
1101	60-64	0.22285094857915055
1101	65-69	0.40404119265740945
1101	70-74	0.31492866491796434
1101	75-79	0.44925607992878724
1101	80-84	0.8889281702612095
1101	85-89	0.3364459320093047
1101	90-94	0.3140610371940724
1101	95-99	1.003353836781315
1101	100-104	0.4797800347744925
1101	105-109	0.5132425586806377
1101	110-114	0.4985440539617727
1101	115-119	0.5598003351800216
1101	120-124	0.7026807307329435
1101	125-129	0.4769493459984062
1101	130-134	0.7107843005718024
1101	135-139	0.2423452972086011
1101	140-144	1.0666652922480075
1101	145-149	0.9821991929087126
1101	150-154	0.8914871769755237
1101	155-159	0.3979798563939525
1101	160-164	0.704293040473793
1101	165-169	0.11879604732546412
1101	170-174	0.34666140850827176
1101	175-179	-0.038061833010647916
1101	180-184	0.13306835766145042
1101	185-189	-0.17415651260504816
1101	190-194	0.2728793139068202
1101	195-199	0.5126658469736185
1101	200-204	0.37238233251996533
1101	205-209	0.3073548799109993
1101	210-214	0.7635153534229566
1101	215-219	0.4593640581483598
1101	220-224	0.31916724778377414
1101	225-229	0.19525091512512915
1101	230-234	0.20766524546630194
1101	235-239	0.09514209138423979
1101	240-244	-0.08013773372078248
1101	245-249	0.143240736826991
1101	250-254	0.09638134741056348
1101	255-259	0.04191134085739279
1101	260-264	0.09444676761416382
1101	265-269	0.1346658952257247
1101	270-274	0.12610076438749473
1101	275-279	0.1375976686044318
1101	280-284	0.059828796786179694
1101	285-289	0.03127757579988533
1101	290-294	0.24967478780043173
1101	295-299	0.13140668123331523
1101	300-301	-0.1193643290068902
1102	1	-0.06548157508444064
1102	2	0.11091579905563975
1102	3	0.21924501925668238
1102	4	0.22880179545038715
1102	5	0.12587687890273713
1102	6	0.19629102794809228
1102	7	1.0495467809499388
1102	8	0.3806362156179759
1102	9	0.22180458526094782
1102	10-14	0.23957288359018492
1102	15-19	0.2587811441316177
1102	20-24	0.27230667756928284
1102	25-29	0.20880288787829926
1102	30-34	0.5351252198310661
1102	35-39	0.23782322074986695
1102	40-44	0.7241121136946802
1102	45-49	0.34852279249425067
1102	50-54	0.3134524908337539
1102	55-59	0.5782497871370538
1102	60-64	0.47219939756992346
1102	65-69	0.40204864454286593
1102	70-74	0.4664826079628739
1102	75-79	0.43459361513454553
1102	80-84	0.8601459961100559
1102	85-89	0.4686699579833231
1102	90-94	0.39175892289271275
1102	95-99	1.0912283869299628
1102	100-104	0.8042059853299719
1102	105-109	0.9843315412342264
1102	110-114	1.020298034168313
1102	115-119	0.7409948372141315
1102	120-124	0.9347350845117006
1102	125-129	0.8479160863363759
1102	130-134	1.0723313002588597
1102	135-139	1.2409116405038567
1102	140-144	1.8071797852850935
1102	145-149	1.8993871818775752
1102	150-154	1.7632748960335753
1102	155-159	1.518290645002935
1102	160-164	1.660809284019404
1102	165-169	1.4415785735341977
1102	170-174	1.527472314469783
1102	175-179	1.2444553297964305
1102	180-184	1.383275680459132
1102	185-189	1.497193823805528
1102	190-194	1.708775251553508
1102	195-199	1.7570945357547174
1102	200-204	1.485521903792062
1102	205-209	1.18890290643267
1102	210-214	1.6304058048390075
1102	215-219	1.400122546647811
1102	220-224	1.3358704241255062
1102	225-229	1.2054354030769368
1102	230-234	0.9512010771045425
1102	235-239	0.7877192995676197
1102	240-244	0.6331113665765074
1102	245-249	0.48745990245052795
1102	250-254	0.7723228569552365
1102	255-259	0.4762486902549856
1102	260-264	0.4923014167301094
1102	265-269	0.43691906374395373
1102	270-274	0.3690911512596582
1102	275-279	0.30141397278218207
1102	280-284	0.225831525232433
1102	285-289	-0.059008324293996495
1102	290-294	0.06334440131944241
1102	295-299	-0.006496962562648534
1102	300-301	-0.16831866909451243
1103	1	-0.029314111338685223
1103	2	0.06389857829044132
1103	3	0.11529851354856646
1103	4	0.050647387088773144
1103	5	-0.042560717518121294
1103	6	0.0787178445170511
1103	7	0.9592317740076339
1103	8	0.2778371181172048
1103	9	0.14476190938930245
1103	10-14	0.10618909000795895
1103	15-19	0.05551922651360286
1103	20-24	0.145595689448335
1103	25-29	-0.01976420175452631
1103	30-34	0.5019641584302619
1103	35-39	0.22512604009574488
1103	40-44	0.5642974345833025
1103	45-49	0.1302013832069946
1103	50-54	0.11661895180259307
1103	55-59	0.42629292582881106
1103	60-64	0.27443202253135013
1103	65-69	0.535854761482085
1103	70-74	0.1931969708138439
1103	75-79	0.4434619235270105
1103	80-84	0.9630010177083221
1103	85-89	0.6696090971379078
1103	90-94	0.5626682680022483
1103	95-99	1.3383454032829363
1103	100-104	0.6928842482117972
1103	105-109	0.9223999421907223
1103	110-114	0.8266478297560909
1103	115-119	0.764618429469607
1103	120-124	1.1590668879674197
1103	125-129	1.1575731173453292
1103	130-134	1.2187820484847123
1103	135-139	1.3349065494825716
1103	140-144	1.6357598917337164
1103	145-149	1.715091729085227
1103	150-154	1.8319405277830363
1103	155-159	0.8259445326919206
1103	160-164	0.9200025307025292
1103	165-169	1.5617276401802904
1103	170-174	1.8763345483482112
1103	175-179	1.8188203984480822
1103	180-184	1.417516655466848
1103	185-189	1.3904675047681962
1103	190-194	1.1359770218404606
1103	195-199	1.1370524962607362
1103	200-204	1.5074827183554724
1103	205-209	0.9204744699933052
1103	210-214	1.5293557816980012
1103	215-219	1.719156023969628
1103	220-224	0.9642721519872772
1103	225-229	0.5156912076125693
1103	230-234	1.2404752208873262
1103	235-239	1.101422149768176
1103	240-244	0.3857417275762014
1103	245-249	0.7959618115835099
1103	250-254	0.6440267099327119
1103	255-259	0.6450391870152465
1103	260-264	0.27142485471221356
1103	265-269	0.4121119054594722
1103	270-274	0.37905769322201444
1103	275-279	0.2743698505976706
1103	280-284	0.41844906225804124
1103	285-289	0.20650627382386766
1103	290-294	0.2802411924332979
1103	295-299	0.12192260315780779
1103	300-301	-0.06938845851136044
2116	1	0.19431320103496574
2116	2	0.17386555918356805
2116	3	0.24892918770017758
2116	4	0.18897303745465166
2116	5	0.1871108874315155
2116	6	0.26513764308668186
2116	7	1.0266423299904517
2116	8	0.4451684650849259
2116	9	0.3517852622332285
2116	10-14	0.27462752111683386
2116	15-19	0.40540294583738046
2116	20-24	0.2799525997441208
2116	25-29	0.3377641085605987
2116	30-34	0.6884834287862844
2116	35-39	0.18735347128291835
2116	40-44	0.6337669644409587
2116	45-49	0.41027985539403744
2116	50-54	0.5644386315587013
2116	55-59	0.6552254001434505
2116	60-64	0.517331993518745
2116	65-69	0.6497098918776274
2116	70-74	0.6998648041249211
2116	75-79	0.8071915628957385
2116	80-84	0.9814479683915067
2116	85-89	0.7150852031858861
2116	90-94	0.8890370412295994
2116	95-99	1.576902693966197
2116	100-104	0.8346564117691955
2116	105-109	0.8686299154346528
2116	110-114	1.024212613059575
2116	115-119	1.0060967828431302
2116	120-124	1.2568499565799343
2116	125-129	1.2862523070186782
2116	130-134	1.4474045945019256
2116	135-139	1.7762794443418066
2116	140-144	2.2382365550079086
2116	145-149	1.7705592234554004
2116	150-154	2.432547950404583
2116	155-159	2.121448960567971
2116	160-164	1.6831740228253729
2116	165-169	1.5292984562634082
2116	170-174	2.1588422505038913
2116	175-179	2.1274477338475997
2116	180-184	2.073840712442074
2116	185-189	1.2939955295624586
2116	190-194	1.754387591638796
2116	195-199	1.0203541306374468
2116	200-204	0.9391999421715944
2116	205-209	0.87292142988683
2116	210-214	2.030532403986129
2116	215-219	1.8496694549633759
2116	220-224	1.6131625349997059
2116	225-229	0.8244286067016695
2116	230-234	1.0663130174030542
2116	235-239	0.5598774818703909
2116	240-244	0.9712243729730972
2116	245-249	1.0015557180155596
2116	250-254	0.8354325317953197
2116	255-259	1.4543936795940056
2116	260-264	0.6967956864529246
2116	265-269	0.3885182108654881
2116	270-274	0.6171088751402554
2116	275-279	0.6494949962363386
2116	280-284	0.47861337491260336
2116	285-289	0.27644276845653515
2116	290-294	0.2693412030252009
2116	295-299	0.11461511099811972
2116	300-301	-0.05802749105613181
1117	1	0.24904585190845552
1117	2	0.03626233615113961
1117	3	0.1577242023663885
1117	4	0.011352851093640481
1117	5	0.2356095150511166
1117	6	0.1021895579401999
1117	7	0.8916578208078718
1117	8	0.23206429375530746
1117	9	0.40710573956244644
1117	10-14	0.27111540529499223
1117	15-19	0.20398980632577945
1117	20-24	0.3579195919339213
1117	25-29	0.3304206364970881
1117	30-34	0.6054764431933322
1117	35-39	0.3243566165667602
1117	40-44	0.6607789447917654
1117	45-49	0.3089319775771173
1117	50-54	0.38739684758198223
1117	55-59	0.45757175832884656
1117	60-64	0.5761775447680222
1117	65-69	0.5482932183392393
1117	70-74	0.5228154808910475
1117	75-79	0.6874815886941334
1117	80-84	1.0670555264967945
1117	85-89	0.7339748119691194
1117	90-94	0.6880446865075527
1117	95-99	1.3355920412314504
1117	100-104	0.6738577217151231
1117	105-109	0.7799286702792116
1117	110-114	1.0607886123056502
1117	115-119	1.008666255919735
1117	120-124	1.2963476535724752
1117	125-129	1.3261069407168051
1117	130-134	1.1970871210954712
1117	135-139	1.314007536320755
1117	140-144	1.7791129936513173
1117	145-149	2.1920531743795486
1117	150-154	2.5323304899562267
1117	155-159	1.841722965444923
1117	160-164	2.1895649568686544
1117	165-169	1.6188353809532963
1117	170-174	1.7070678156536765
1117	175-179	1.986265955289717
1117	180-184	1.8771233521244817
1117	185-189	1.6571622531662236
1117	190-194	-0.2791842492357546
1117	195-199	1.6346330124713688
1117	200-204	2.0476884389144026
1117	205-209	1.9235223322416708
1117	210-214	2.2523597314372736
1117	215-219	1.8765728228909069
1117	220-224	2.1789090981586554
1117	225-229	2.007522764955386
1117	230-234	1.47261583637842
1117	235-239	1.0197045501595703
1117	240-244	1.2073221005938706
1117	245-249	1.1126110962153284
1117	250-254	0.877957476134398
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2108	265-269	-0.5174060385132826
2108	270-274	-0.452352332682171
2108	275-279	-0.2678406856029092
2108	280-284	-0.8513653483473291
2108	285-289	-1.1091699948742146
2108	290-294	-0.9405091814710431
2108	295-299	-0.598837848413277
2108	300-301	2.5350797269818397
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	35.0
11	222.0
12	461.0
13	570.0
14	1058.0
15	2230.0
16	3528.0
17	4872.0
18	4806.0
19	5773.0
20	7321.0
21	9209.0
22	11965.0
23	15622.0
24	20886.0
25	27927.0
26	37151.0
27	46890.0
28	51864.0
29	46706.0
30	30989.0
31	13148.0
32	3237.0
33	321.0
34	10.0
35	0.0
36	0.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.257436477087925	20.375774212523503	14.453985536499003	34.91280377388957
2	47.78606936482855	23.714259351103216	15.165049999423305	13.334621284644928
3	47.378346270515905	22.30308762298013	17.528056193123494	12.790509913380468
4	47.758676370514756	21.00004613556937	17.52142420502647	13.719853288889402
5	29.309350526522188	21.02916921373456	36.500444054855194	13.161036204888063
6	28.47400837360584	20.633556706381704	36.60396073863046	14.288474181381991
7	15.945029469094935	50.95442959135419	18.992283826022767	14.108257113528103
8	27.275925306513187	19.70796184588413	38.099906575472026	14.916206272130655
9	14.917936355982054	18.102444031787407	39.03645863369511	27.943160978535424
10-14	27.021833658204635	35.188579139802314	22.78664605944568	15.002941142547375
15-19	21.19041302868479	36.47207067969227	20.874442047957924	21.46307424366501
20-24	25.627732090748665	31.563822793278046	26.10950277390111	16.69894234207218
25-29	18.57227713636521	25.446476972583937	39.53114727627132	16.450098614779527
30-34	19.011026401079572	28.510224795561754	31.912665367181464	20.56608343617721
35-39	20.252476903380582	30.76913761086954	27.64847002918075	21.32991545656913
40-44	19.802424424170425	29.01973448979827	29.76978350884073	21.408057577190576
45-49	20.61700557087	31.425992779783396	25.946125188867487	22.010876460479118
50-54	21.722644490836323	30.02491320746012	27.581400445208242	20.67104185649531
55-59	21.460536787349625	26.66930023875157	29.91557190805181	21.95459106584699
60-64	21.369649715689555	26.46163250712218	28.6188163919678	23.54990138522047
65-69	19.667535553223146	26.716819875203285	29.986390007035673	23.629254564537895
70-74	20.431367573615066	26.084877913749555	29.533511724201567	23.950242788433812
75-79	20.125373409764595	26.00771617397723	31.047738780406224	22.81917163585195
80-84	23.615356224265003	25.10311299754328	27.180309338992632	24.101221439199087
85-89	20.229063101925007	29.123366512496972	25.947451586486892	24.70011879909113
90-94	19.61499867360238	31.135627040057205	28.82596509844177	20.42340918789864
95-99	21.559555253111267	30.68280642668481	26.267344090610255	21.490294229593662
100-104	22.132039999538645	29.06535103401345	26.148891016251252	22.653717950196654
105-109	23.530928132316813	27.915825653683346	23.314898328739	25.238347885260836
110-114	20.853796380664583	28.315129006585853	29.098049618804854	21.733024993944706
115-119	23.101232973091427	28.38738884211255	26.37720441517399	22.134173769622034
120-124	18.29125384943657	29.009007969919608	27.895006978004865	24.804731202638955
125-129	20.244864534434438	24.776703844246317	30.938051464227634	24.040380157091615
130-134	24.73374009526995	28.99003471701595	25.372141036435565	20.904084151278532
135-139	23.80278197483305	27.664386800613606	24.306697731283375	24.226133493269973
140-144	20.495553684501907	29.268981903322917	26.527779379707273	23.707685032467907
145-149	19.639392855907083	27.735377907982606	28.99891581411979	23.62631342199052
150-154	22.892123504919205	25.44382417734513	25.46694963149214	26.19710268624353
155-159	21.499636682391206	27.11266306040299	29.775377446626912	21.612322810578885
160-164	24.393028915468104	28.995917002110705	26.190355359223076	20.420698723198115
165-169	18.93224991637928	25.334136861166538	28.68974982987509	27.04386339257909
170-174	21.28176145603857	29.02192592934337	26.534411367804296	23.161901246813763
175-179	19.818225856679852	28.84332360641746	28.507975686554943	22.830474850347745
180-184	19.033517491147737	29.438299442913003	26.69323306536257	24.834950000576693
185-189	21.75759218463455	27.058280758007403	28.059018927117336	23.12510813024071
190-194	19.146607305567407	27.606486661053502	27.83531908513166	25.41158694824743
195-199	20.11678065996932	29.564364886218154	27.611965259916264	22.706889193896266
200-204	21.71070691226168	26.992883588424586	27.905041464342972	23.39136803497076
205-209	20.41262499855826	27.112259374171	28.877348588828273	23.597767038442463
210-214	22.26687120102421	26.80839898040392	28.1376800728942	22.787049745677674
215-219	21.952918651457306	26.895191520282353	28.819563788191598	22.332326040068743
220-224	18.08583522681399	26.69432878513512	28.954741006447442	26.265094981603443
225-229	20.587767153781385	28.147426211923737	27.10799183400422	24.156814800290654
230-234	20.076873392463753	26.5514238590097	29.846022537225636	23.52568021130091
235-239	20.406050679922956	26.498829309927217	26.987347320100113	26.107772690049714
240-244	21.7586879044071	30.940415912157874	23.77492762482555	23.525968558609474
245-249	19.398392175407434	28.90347285498437	25.25795550224334	26.44017946736485
250-254	20.402821190067012	26.653556475703855	25.966194161543697	26.977428172685435
255-259	20.372948408899553	26.774085650684537	28.36057254241589	24.492393398000022
260-264	20.118799091129283	27.905906506268668	27.733186468437506	24.24210793416454
265-269	19.377919516499233	27.596855860947393	27.813058672910344	25.21216594964303
270-274	18.57250781421206	29.668573603533982	25.707431286836368	26.051487295417587
275-279	19.66563246098661	28.534561308404747	24.414943310919135	27.384862919689507
280-284	16.985444227863578	29.28455265798549	28.464838929193437	25.2651641849575
285-289	19.623591423397656	27.34622438034163	28.37268312937567	24.657501066885043
290-294	18.454919781778756	29.727800140713484	26.743982191670224	25.07329788583753
295-299	18.01680488114324	31.128476026804762	23.858952030541747	26.995767061510247
300-301	13.6882792586014	31.707390918213168	32.12016009042571	22.484169732759714
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.25
24	0.5
25	0.25
26	0.25
27	0.25
28	1.75
29	3.0
30	1.0
31	3.25
32	6.0
33	18.75
34	46.25
35	152.75
36	513.5
37	1425.75
38	2871.75
39	4631.5
40	6475.75
41	8573.25
42	11144.25
43	13375.25
44	14025.75
45	12375.0
46	9572.25
47	7466.25
48	6083.5
49	5080.5
50	4294.0
51	3274.25
52	2017.75
53	951.75
54	430.75
55	217.25
56	110.75
57	83.0
58	62.0
59	43.0
60	39.0
61	38.0
62	34.75
63	27.25
64	26.5
65	20.25
66	12.25
67	8.25
68	6.25
69	5.0
70	4.25
71	3.25
72	1.5
73	2.5
74	1.0
75	2.75
76	3.25
77	1.25
78	2.0
79	1.5
80	1.0
81	0.75
82	0.25
83	0.0
84	1.0
85	0.25
86	0.5
87	1.0
88	0.75
89	0.5
90	0.0
91	0.25
92	0.75
93	0.25
94	0.75
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	346804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.558097771326231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.66848751721224	8.660957715746418
2	4.450125215487534	1.3847096639439858
3	4.074909214698594	1.9019350791467673
4	4.032584445139415	2.5095737227443347
5	3.3832318395843415	2.6318325871658543
6	3.536238837005279	3.3010289741333456
7	2.863551115586316	3.1185985760637545
8	2.8736859446836447	3.57672695131793
9	2.75808568125844	3.861951002164235
>10	16.13795336602232	40.507643894456926
>50	0.1561991926464307	1.5110218532742505
>100	0.021104038203061677	0.46740911611380404
>500	0.009531215754917437	0.984036424641125
>1k	0.022874917811609618	10.069913430382183
>5k	0.009531215754837341	11.321757236403364
>10k+	0.0019062431509674683	4.190903772301662
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	14131	4.0746358173492805	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	9719	2.8024474919551103	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	8030	2.3154288877867613	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	7889	2.2747719172789242	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	6806	1.9624917821017058	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5731	1.6525184253930174	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4872	1.4048280873346328	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	4621	1.332452912884511	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4199	1.2107703486695656	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3900	1.1245545034082653	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	3724	1.0738053771006102	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2724	0.7854580685343883	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2707	0.7805561642887625	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1822	0.5253687962076562	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1715	0.4945156341910705	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT	1466	0.4227171543580812	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1121	0.32323733290273465	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1083	0.31228013517721825	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	840	0.24221173919562636	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	657	0.18944418172800775	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	615	0.17733359476822644	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	609	0.1756035109168291	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	597	0.17214334321403443	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.766946171324437E-4	0.0	0.0
2	8.650419256986655E-4	0.0	0.0
3	0.0011533892342648873	0.0	0.0
4	0.0011533892342648873	0.0	0.0
5	0.0011533892342648873	0.0	0.0
6	0.0011533892342648873	0.0	0.0
7	0.0011533892342648873	0.0	0.0
8	0.002018431159963553	0.0	0.0
9	0.0023067784685297747	0.0	0.0
10-14	0.004555887475346305	0.0	0.0
15-19	0.005247921015905237	0.0	0.0
20-24	0.006516649173596614	0.0	0.0
25-29	0.006689657558736346	0.0	0.0
30-34	0.014705712736877314	0.0	0.0
35-39	0.015628424124289223	0.0	0.0
40-44	0.017358507975686555	0.0	0.0
45-49	0.021222361910473924	0.0	0.0
50-54	0.030795492554872496	0.0	0.0
55-59	0.040022606428991594	0.0	0.0
60-64	0.05115281253964775	0.0	0.0
65-69	0.06770394805134888	0.0	0.0
70-74	0.08858029319154334	0.0	0.0
75-79	0.11637697373732714	0.0	0.0
80-84	0.15161301484411943	0.0	0.0
85-89	0.1912896045028316	0.0	0.0
90-94	0.23961661341853033	0.0	0.0
95-99	0.2896737061856265	0.0	0.0
100-104	0.35063032721652576	0.0	0.0
105-109	0.4207563926598309	0.0	0.0
110-114	0.48528852031695136	0.0	0.0
115-119	0.5625079295509856	0.0	0.0
120-124	0.6557594491413017	0.0	0.0
125-129	0.7458968177991027	0.0	0.0
130-134	0.8475680787995525	0.0	0.0
135-139	0.9587548009826875	0.0	0.0
140-144	1.0822251185107439	0.0	0.0
145-149	1.2190170816945594	0.0	0.0
150-154	1.3731675528540617	0.0	0.0
155-159	1.557536821951304	0.0	0.0
160-164	1.7491147737627017	0.0	0.0
165-169	1.9635875018742575	0.0	0.0
170-174	2.223965121509556	0.0	0.0
175-179	2.480940242903773	0.0	0.0
180-184	2.742586590696763	0.0	0.0
185-189	3.0873346328185374	0.0	0.0
190-194	3.3999031153043218	0.0	0.0
195-199	3.705032237229098	0.0	0.0
200-204	3.999319500351784	0.0	0.0
205-209	4.302776207886876	0.0	0.0
210-214	4.585183561896633	0.0	0.0
215-219	4.891466073055674	0.0	0.0
220-224	5.1912319350411185	0.0	0.0
225-229	5.476349753751398	0.0	0.0
230-234	5.744166733947705	0.0	0.0
235-239	5.983379661134243	0.0	0.0
240-244	6.20800221450733	0.0	0.0
245-249	6.369592046227841	0.0	0.0
250-254	6.528644421632968	0.0	0.0
255-259	6.656382279327805	0.0	0.0
260-264	6.775181370457088	0.0	0.0
265-269	6.876448945225545	0.0	0.0
270-274	6.952860981995594	0.0	0.0
275-279	7.0066089203123365	0.0	0.0
280-284	7.04397873150252	0.0	0.0
285-289	7.073159479129423	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6270	0.0	292.17703	1
GGGTTAT	6330	0.0	289.4076	2
GTTATTA	6325	0.0	287.07114	4
GGTTATT	6430	0.0	283.53033	3
TTATTAG	6415	0.0	282.5838	5
GGGGGGA	4165	0.0	277.64706	1
TTAGTAG	6570	0.0	277.26407	8
ATTAGTA	6715	0.0	269.30005	7
GGGGAGC	4330	0.0	269.11084	3
TATTAGT	6725	0.0	268.89963	6
GGGAGCG	3510	0.0	266.4245	4
GGAGCGA	3500	0.0	265.92142	5
TAGTAGA	6870	0.0	264.51236	9
CGAAAGC	3705	0.0	259.17004	9
AGCGAAA	3725	0.0	250.25504	7
GAGCGAA	3735	0.0	249.58502	6
GCGAAAG	3875	0.0	248.5613	8
GGGGGAG	4830	0.0	242.4741	2
GGGAGCA	1135	0.0	204.03084	4
GTGCGAA	105	0.0	168.57144	6
>>END_MODULE