##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_8033_flu.3520000003ba76.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440515
Sequences flagged as poor quality	0
Sequence length	301
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94623792606381	34.0	34.0	34.0	33.0	34.0
2	33.201523217143574	34.0	34.0	34.0	34.0	34.0
3	33.21747500085127	34.0	34.0	34.0	34.0	34.0
4	33.356230775342496	34.0	34.0	34.0	34.0	34.0
5	33.243333371167836	34.0	34.0	34.0	34.0	34.0
6	36.77779190265939	38.0	38.0	38.0	36.0	38.0
7	35.92516032371202	38.0	37.0	38.0	33.0	38.0
8	36.67274894157974	38.0	38.0	38.0	34.0	38.0
9	36.797062529085274	38.0	38.0	38.0	36.0	38.0
10-14	36.822226711916734	38.0	38.0	38.0	36.0	38.0
15-19	36.63995459859483	38.0	38.0	38.0	35.4	38.0
20-24	36.70162423527008	38.0	38.0	38.0	35.2	38.0
25-29	36.67146317378523	38.0	38.0	38.0	35.4	38.0
30-34	36.322771755785844	38.0	37.4	38.0	33.0	38.0
35-39	36.5947407012247	38.0	38.0	38.0	34.8	38.0
40-44	36.05923430530174	38.0	37.2	38.0	32.2	38.0
45-49	36.448441483263906	38.0	37.2	38.0	34.2	38.0
50-54	36.377207132560756	38.0	37.0	38.0	34.0	38.0
55-59	36.03870288185419	38.0	37.0	38.0	32.8	38.0
60-64	36.236465500607245	38.0	37.0	38.0	33.6	38.0
65-69	36.09943361747047	38.0	37.0	38.0	33.0	38.0
70-74	35.93127453094674	38.0	37.0	38.0	31.8	38.0
75-79	35.9335559515567	38.0	37.0	38.0	31.8	38.0
80-84	35.19986061768611	38.0	36.6	38.0	28.4	38.0
85-89	35.38880855362474	38.0	37.0	38.0	29.0	38.0
90-94	35.4938419804093	38.0	37.0	38.0	29.8	38.0
95-99	34.557768520935724	38.0	35.2	38.0	24.4	38.0
100-104	34.87616721337526	38.0	36.0	38.0	25.2	38.0
105-109	34.70073936188325	38.0	35.6	38.0	25.0	38.0
110-114	34.26255133196372	38.0	35.0	38.0	23.8	38.0
115-119	34.19094877586461	37.6	34.6	38.0	23.6	38.0
120-124	33.59997185112879	37.0	33.0	38.0	22.0	38.0
125-129	33.136606017956254	37.0	31.6	38.0	21.8	38.0
130-134	33.141123457771016	37.0	32.0	38.0	21.8	38.0
135-139	32.62365413209539	37.0	30.6	38.0	20.0	38.0
140-144	31.20962827599514	35.8	26.8	37.8	14.6	38.0
145-149	31.01068158859517	35.0	27.2	37.6	15.0	38.0
150-154	30.23588345459292	35.0	24.8	37.0	10.8	38.0
155-159	29.828782220809735	35.0	24.0	37.0	10.0	37.2
160-164	29.288985846111938	34.2	23.2	37.0	10.2	37.4
165-169	27.967325970738795	32.2	21.2	36.6	9.6	37.0
170-174	27.475517973281274	31.4	20.4	36.2	9.2	37.0
175-179	26.772524885645215	29.4	20.0	35.6	9.2	37.0
180-184	25.63728317991442	27.6	18.6	35.0	9.0	36.8
185-189	25.09378114252636	26.0	17.0	35.0	9.0	36.4
190-194	24.06519777987129	25.2	15.2	35.0	8.4	36.0
195-199	23.278425479268584	24.6	14.2	34.4	8.0	36.0
200-204	22.409749951761007	23.2	11.8	33.0	8.0	36.0
205-209	21.854295086432927	22.6	9.4	31.6	8.0	35.8
210-214	20.673560718704245	20.4	9.0	29.2	8.0	35.0
215-219	20.22190663201026	20.0	9.0	28.4	8.0	35.0
220-224	19.54160108055344	19.6	9.0	27.4	8.0	34.6
225-229	18.86903238255224	18.0	9.0	26.0	8.0	34.0
230-234	18.368611284519254	17.4	9.0	25.8	8.0	33.4
235-239	17.38800131664075	15.4	9.0	24.8	8.0	31.4
240-244	16.6951127657401	15.0	8.6	23.2	7.6	31.0
245-249	15.964025742596732	13.8	8.2	22.2	7.2	29.2
250-254	15.14583385355777	12.8	8.0	20.8	7.0	27.6
255-259	14.53511548982441	12.2	8.0	19.6	7.0	26.0
260-264	14.114849210583069	11.6	8.0	19.0	7.0	25.4
265-269	13.579742801039695	11.0	8.0	17.8	7.0	24.6
270-274	13.011078396876382	9.8	8.0	16.4	7.0	23.4
275-279	12.535553159370284	9.0	8.0	15.6	7.0	22.6
280-284	12.17820823354483	9.0	8.0	14.8	7.0	21.6
285-289	11.790384436398305	9.0	7.8	14.0	7.0	21.0
290-294	11.678344664767375	8.8	7.4	14.0	7.0	20.6
295-299	11.28383914282147	8.0	7.0	13.2	7.0	19.4
300-301	10.977623917460246	8.0	7.0	13.0	7.0	17.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.17413201769922892
1101	2	0.242939089871534
1101	3	0.23949656266024988
1101	4	0.16114796972030376
1101	5	0.24531167995379377
1101	6	0.2837980439132579
1101	7	1.0745968151944183
1101	8	0.356829066701259
1101	9	0.26845895292483846
1101	10-14	0.2069014705774137
1101	15-19	0.269246751042985
1101	20-24	0.22558827185147123
1101	25-29	0.25139776635532485
1101	30-34	0.590887101528665
1101	35-39	0.31440577111225565
1101	40-44	0.6505525681081323
1101	45-49	0.32533281286517024
1101	50-54	0.39193199495318964
1101	55-59	0.5336671238762065
1101	60-64	0.39684302323426124
1101	65-69	0.3982760173925257
1101	70-74	0.3579959328967135
1101	75-79	0.4478014206736276
1101	80-84	0.8949405166138718
1101	85-89	0.45320676765532397
1101	90-94	0.5408176235337621
1101	95-99	1.0744992109656835
1101	100-104	0.6660177066546638
1101	105-109	0.8072390306279047
1101	110-114	0.8387605748265372
1101	115-119	0.6689827012171463
1101	120-124	1.1367367650690525
1101	125-129	0.833594644340323
1101	130-134	1.0540158172265564
1101	135-139	0.9141633803050269
1101	140-144	1.3368154510297074
1101	145-149	1.3438365081720676
1101	150-154	1.3417667644547393
1101	155-159	0.8879557508442133
1101	160-164	1.2205372633173823
1101	165-169	0.8355740589010772
1101	170-174	0.8347014785231259
1101	175-179	1.0728256915744758
1101	180-184	0.8049758839656356
1101	185-189	0.580282235117032
1101	190-194	0.9210848125463471
1101	195-199	1.1171951582919455
1101	200-204	0.8941231305860917
1101	205-209	0.8675701712164781
1101	210-214	0.9368998891677371
1101	215-219	0.7282372468879927
1101	220-224	0.8105920432298497
1101	225-229	0.6624332959311623
1101	230-234	0.7626353183301404
1101	235-239	0.37522045750536037
1101	240-244	0.2920318228024392
1101	245-249	0.2844207484685537
1101	250-254	0.5405618349031887
1101	255-259	0.24201494651418365
1101	260-264	0.10829376811784641
1101	265-269	0.10964010003423219
1101	270-274	0.06961349975952125
1101	275-279	0.2337030083766347
1101	280-284	0.15950861008719386
1101	285-289	0.08826136040462629
1101	290-294	-0.016235634062113036
1101	295-299	0.07089355836513711
1101	300-301	-0.2179106406571858
1102	1	-0.1914613524548372
1102	2	0.027145273913319556
1102	3	0.13026322351470299
1102	4	-0.010700245515685936
1102	5	0.11255851097772052
1102	6	0.1482482200534534
1102	7	1.1040458553115116
1102	8	0.35238989602077453
1102	9	0.2586589609260699
1102	10-14	0.21074553121462714
1102	15-19	0.2702213909312121
1102	20-24	0.22715288892111118
1102	25-29	0.17735412042315346
1102	30-34	0.5163568904850209
1102	35-39	0.23311422229382828
1102	40-44	0.49022943935708696
1102	45-49	0.17741264094448894
1102	50-54	0.19981018308314447
1102	55-59	0.39327640741171876
1102	60-64	0.2877473655599161
1102	65-69	0.2894398711754036
1102	70-74	0.2070922095338119
1102	75-79	0.4695117657302674
1102	80-84	0.8926357106700138
1102	85-89	0.49788244249457136
1102	90-94	0.44069360453311646
1102	95-99	1.0889750891568326
1102	100-104	0.7778971870580804
1102	105-109	0.8846336805252122
1102	110-114	0.9396659965628515
1102	115-119	0.8726412239946058
1102	120-124	1.3134850517876941
1102	125-129	0.965539387572683
1102	130-134	1.100644696971905
1102	135-139	1.2355892289444697
1102	140-144	1.8033450400912798
1102	145-149	1.7576503981491811
1102	150-154	1.767743423520237
1102	155-159	1.3461307014215471
1102	160-164	1.5411694809326129
1102	165-169	1.2654136494194717
1102	170-174	1.3820985514331667
1102	175-179	1.3434787803118482
1102	180-184	1.2831107227371525
1102	185-189	1.2498746330466801
1102	190-194	1.459490168234808
1102	195-199	1.4747463906694342
1102	200-204	1.3557932581603538
1102	205-209	0.9716578163424856
1102	210-214	1.887834107768775
1102	215-219	1.9082408247946425
1102	220-224	1.4167893700655831
1102	225-229	1.2262312211982014
1102	230-234	1.304823744179533
1102	235-239	0.7586321047237625
1102	240-244	0.9855293171355335
1102	245-249	0.7631834570193714
1102	250-254	1.1331027298478489
1102	255-259	0.7113205080454978
1102	260-264	0.7254566994952576
1102	265-269	0.7327275617359117
1102	270-274	0.6092954283870764
1102	275-279	0.6644102035167183
1102	280-284	0.47335775074799
1102	285-289	0.48246605742613013
1102	290-294	0.5127543923343865
1102	295-299	0.33950391904681254
1102	300-301	0.15063789923232207
1103	1	-0.01994979257057139
1103	2	0.13763012555473608
1103	3	0.23340430852621807
1103	4	0.12981637703101256
1103	5	0.08517242843073092
1103	6	0.24376323103249575
1103	7	1.102447119807124
1103	8	0.4543051328457324
1103	9	0.3459176126289307
1103	10-14	0.1748736202728125
1103	15-19	0.3183329129228838
1103	20-24	0.2558754781177939
1103	25-29	0.1469591240576804
1103	30-34	0.5903649083171842
1103	35-39	0.2674083820783082
1103	40-44	0.6862357708931626
1103	45-49	0.2430003498538582
1103	50-54	0.22900771296885836
1103	55-59	0.5216566800119793
1103	60-64	0.3865732234083197
1103	65-69	0.43395922017755595
1103	70-74	0.4013379694502319
1103	75-79	0.47895894895909663
1103	80-84	0.9953756776234499
1103	85-89	0.6457219982906608
1103	90-94	0.5570056130898919
1103	95-99	1.4053956426454022
1103	100-104	0.9090116144005336
1103	105-109	0.9167255406366124
1103	110-114	0.8612148829205282
1103	115-119	0.8686345724094906
1103	120-124	1.391392987871491
1103	125-129	1.2067887261505987
1103	130-134	1.191004503040304
1103	135-139	1.2689066353093708
1103	140-144	1.9444742847982042
1103	145-149	1.680128936370508
1103	150-154	2.2897215077097464
1103	155-159	1.1041437404003496
1103	160-164	1.0788488386002406
1103	165-169	1.813990072826229
1103	170-174	2.1025865960165966
1103	175-179	1.7647316967964066
1103	180-184	1.372251776045701
1103	185-189	1.5515616084851764
1103	190-194	1.689753219857053
1103	195-199	1.803879231398998
1103	200-204	1.421364209785395
1103	205-209	1.420572782182532
1103	210-214	1.735681438340933
1103	215-219	1.970360832614709
1103	220-224	1.4527156289565717
1103	225-229	1.0445046623367311
1103	230-234	1.7151157360847122
1103	235-239	1.2739149744766394
1103	240-244	0.7599169402959092
1103	245-249	0.8929499042561932
1103	250-254	1.0084469523966568
1103	255-259	0.9036337454697954
1103	260-264	0.6384939421822509
1103	265-269	0.8241744777539886
1103	270-274	0.9155665023704849
1103	275-279	0.6549388656438229
1103	280-284	0.6855312384596921
1103	285-289	0.4104545718928776
1103	290-294	0.22867298212587883
1103	295-299	0.18177258360447723
1103	300-301	0.16503104776753297
2116	1	0.09483452378032808
2116	2	-0.1267223771789645
2116	3	-8.595998891252066E-4
2116	4	-0.047604345559420835
2116	5	-0.034037140648287334
2116	6	-0.1850198802571441
2116	7	0.7145646802275607
2116	8	0.06740905822120169
2116	9	-0.1892947236277891
2116	10-14	0.006661350963931056
2116	15-19	0.013263621900591716
2116	20-24	0.07829854388289448
2116	25-29	-0.013563515472796439
2116	30-34	0.34951958016115015
2116	35-39	-0.213438731353655
2116	40-44	0.15980386801217605
2116	45-49	-0.03547725609111296
2116	50-54	0.034016304609302495
2116	55-59	0.2135644705098514
2116	60-64	0.12745559604004342
2116	65-69	0.2417269557781836
2116	70-74	0.1856386991817729
2116	75-79	0.29820154564294654
2116	80-84	0.588101570819326
2116	85-89	0.3731067922028757
2116	90-94	0.4511802577084083
2116	95-99	1.0565728089661732
2116	100-104	0.49134630900677223
2116	105-109	0.46436652164573644
2116	110-114	0.5546629202327935
2116	115-119	0.673979242245025
2116	120-124	0.7929774202653235
2116	125-129	0.7958976502986843
2116	130-134	0.7823972414304308
2116	135-139	1.1983266437894287
2116	140-144	1.6145677886253935
2116	145-149	1.6452629436455055
2116	150-154	2.056461817008799
2116	155-159	1.830047647923056
2116	160-164	1.7182440763766778
2116	165-169	1.7617078207006358
2116	170-174	1.794899197833118
2116	175-179	1.8742195457620667
2116	180-184	2.215035467550017
2116	185-189	1.4181541862004714
2116	190-194	1.5900183333336528
2116	195-199	0.7090301987953964
2116	200-204	1.027631420961221
2116	205-209	0.7427142206686206
2116	210-214	1.9990730163017005
2116	215-219	1.7781147323268343
2116	220-224	1.4707321873908832
2116	225-229	0.7623989293693789
2116	230-234	1.1805858215547929
2116	235-239	1.1543969989795464
2116	240-244	1.0700349916672245
2116	245-249	0.9258056762764593
2116	250-254	0.8474833497098189
2116	255-259	1.5635310921034336
2116	260-264	0.6733719855198252
2116	265-269	0.551524012466448
2116	270-274	0.6454935292165711
2116	275-279	0.8373596998140052
2116	280-284	0.42908750812562246
2116	285-289	0.2852866443750788
2116	290-294	0.2930027225913907
2116	295-299	0.20304370007141692
2116	300-301	0.1764623927497908
1117	1	0.10561858099295307
1117	2	0.10159612971096976
1117	3	0.1722042410318565
1117	4	0.1650155742033519
1117	5	0.1552311307628429
1117	6	0.11802390135806462
1117	7	0.9807996357482267
1117	8	0.15291839015910114
1117	9	0.1953119395503009
1117	10-14	0.11070024857425409
1117	15-19	0.1799486035855722
1117	20-24	0.21876601292179032
1117	25-29	0.2671603638225122
1117	30-34	0.479152407651128
1117	35-39	0.20575627998420742
1117	40-44	0.5889413178639131
1117	45-49	0.12288747441647985
1117	50-54	0.29921428123585514
1117	55-59	0.31111113019935743
1117	60-64	0.3649856849987074
1117	65-69	0.46180108799517683
1117	70-74	0.3948616191189771
1117	75-79	0.47825736284161735
1117	80-84	1.0057502713251623
1117	85-89	0.5685550740797254
1117	90-94	0.4627222078217912
1117	95-99	1.131864977115491
1117	100-104	0.6023527628703675
1117	105-109	0.5846142104648564
1117	110-114	0.8097824039237125
1117	115-119	0.7403886265115531
1117	120-124	1.0097705477727317
1117	125-129	1.145578445693765
1117	130-134	0.8944502677691659
1117	135-139	1.23659282205562
1117	140-144	1.4302960286393471
1117	145-149	1.8797719267259154
1117	150-154	2.1463754747795996
1117	155-159	1.5946403568014773
1117	160-164	1.7344406575732627
1117	165-169	1.2463598353526457
1117	170-174	1.1470778128688721
1117	175-179	1.4550751187604938
1117	180-184	1.7647581258441107
1117	185-189	1.33893039411954
1117	190-194	-0.7196258320247964
1117	195-199	1.176235850286762
1117	200-204	1.6380544106699304
1117	205-209	1.8053576883120925
1117	210-214	2.1154571084000757
1117	215-219	1.8321257213378246
1117	220-224	1.685406877779858
1117	225-229	1.3503145058563852
1117	230-234	1.2919864843840294
1117	235-239	0.9109747069858294
1117	240-244	0.8505076935684954
1117	245-249	1.1335197875619993
1117	250-254	0.7256788843339272
1117	255-259	0.7860097245820672
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2108	250-254	-0.7739411045066831
2108	255-259	-0.8178151856637221
2108	260-264	-0.6096528199242037
2108	265-269	-0.27935101143260077
2108	270-274	-0.6833506064875454
2108	275-279	-0.4233158466776157
2108	280-284	-0.9391766772885219
2108	285-289	-1.3257309948944247
2108	290-294	-0.9349433753132832
2108	295-299	-0.7984831482988461
2108	300-301	2.3940021713914756
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	14.0
10	288.0
11	930.0
12	948.0
13	866.0
14	983.0
15	1614.0
16	2513.0
17	3815.0
18	4824.0
19	5584.0
20	7391.0
21	9506.0
22	12776.0
23	17456.0
24	23357.0
25	31424.0
26	41959.0
27	52610.0
28	60909.0
29	62736.0
30	52506.0
31	31996.0
32	11362.0
33	2041.0
34	101.0
35	3.0
36	2.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.468917062983103	21.370668422187666	15.740213159597289	33.420201355231946
2	28.91411189176305	25.429100030645944	15.850084560117136	29.806703517473863
3	44.959422494126194	25.144433220208168	18.222307980431996	11.673836305233646
4	45.96392858358966	23.416228732279265	18.56803968082812	12.051803003302952
5	44.39508302781971	23.683188994699385	19.459042257357865	12.462685720123037
6	26.591830017139028	23.524511083618037	36.22759724413471	13.656061655108227
7	16.527019511253872	34.00587948196997	36.13724844783946	13.3298525589367
8	25.615245791857262	37.84229821912988	21.858052506725084	14.684403482287777
9	14.229708409475272	20.590899288332974	39.5355436250752	25.643848677116555
10-14	23.22622385162821	35.04257516770144	27.341225610932657	14.389975369737693
15-19	23.346764582363825	33.82168598118112	22.1145704459553	20.716978990499758
20-24	22.09931557381701	30.1471232534647	27.695084162854837	20.058477009863456
25-29	17.495703892035458	28.60983167429032	33.44489971964632	20.449564714027897
30-34	17.69297299751427	30.65702643496816	31.440881695288468	20.209118872229094
35-39	20.361667593612022	31.040577505873806	30.666878539890806	17.93087636062336
40-44	19.052291068408568	29.78958718772346	31.38226848120949	19.77585326265848
45-49	20.807918005062255	28.622180856497508	31.441290307935034	19.128610830505206
50-54	18.942283463673203	31.751699715106184	30.835771767136194	18.470245054084426
55-59	21.09998524454332	31.7819824523569	27.554884623679104	19.563147679420677
60-64	19.443787385219572	30.43351531729907	28.684607788611054	21.438089508870302
65-69	18.608855544079088	31.335459632475626	29.833762754957267	20.22192206848802
70-74	19.72395945654518	32.45442266438146	26.45040464002361	21.371213239049748
75-79	19.617084548766783	33.53506691032088	27.60364573283543	19.24420280807691
80-84	20.128712983666844	32.12494466703745	25.610206235883	22.13613611341271
85-89	20.70481141391326	32.58032076092755	25.422289819869924	21.292578005289265
90-94	19.474433333711676	30.324869754718907	29.106023631431395	21.09467328013802
95-99	20.209255076444617	29.837077057534934	28.449791721053767	21.503876144966686
100-104	19.309081416069827	28.9599219095831	29.342701156600796	22.388295517746272
105-109	20.105603668433538	28.272998649308196	31.336004449337707	20.28539323292056
110-114	18.860016117498837	29.187882364959194	28.81813332122629	23.133968196315678
115-119	18.22979921228562	30.05168949979002	29.911081347967723	21.80742993995664
120-124	21.604803468667356	29.08790847076717	28.36112277674994	20.946165283815535
125-129	21.62346344619366	28.93790222807396	29.284768963599422	20.153865362132958
130-134	21.334574305074742	29.446897381473956	28.31422312520572	20.904305188245576
135-139	20.1466011373052	27.614587471482245	30.785194601772925	21.453616789439632
140-144	20.10496804876111	28.952793888970863	29.19505578697661	21.747182275291422
145-149	19.55497542648945	30.656118406864692	27.973394776568334	21.81551139007752
150-154	19.549845067704847	28.461051269536792	28.43412823626891	23.55497542648945
155-159	20.40715980159586	29.59236348364982	30.33780915519335	19.66266755956097
160-164	20.844193727795876	28.3045072244986	28.07037217801891	22.780926869686617
165-169	21.321453298979602	28.13148246938243	28.804876110915632	21.742188120722336
170-174	19.599378000749123	30.30834364323576	30.774797679988197	19.317480676026925
175-179	20.280535282566994	31.488916381962024	27.698126057001467	20.532422278469518
180-184	22.02077114286687	27.823252329659603	29.3531434797907	20.802833047682824
185-189	20.626039975937253	29.704050940376604	27.976618276335653	21.693290807350486
190-194	20.84850686128736	29.31382586291046	28.432085173036103	21.40558210276608
195-199	20.284939218868825	30.12791845907631	30.544385548732734	19.042756773322132
200-204	21.667729816237813	27.64732188461233	29.023529278231162	21.6614190209187
205-209	21.03565145341248	28.07713698738976	29.875895258958263	21.011316300239493
210-214	21.346469473230194	26.75927039941886	29.1073856735866	22.786874453764344
215-219	20.269366536894317	29.267380225417977	28.232545997298615	22.230707240389087
220-224	21.20018614576121	27.982565860413377	30.260989977639806	20.5562580161856
225-229	19.926812934860333	29.38369862547246	29.20926642679591	21.480222012871298
230-234	18.671100870571944	30.15901842162015	27.47193625642714	23.69794445138077
235-239	20.770734254225168	29.294575667116895	27.315437612794113	22.619252465863816
240-244	19.460358898107895	29.401995391757374	28.886008421960657	22.25163728817407
245-249	21.268379056331792	29.206360736864806	27.72087216099338	21.804388045810015
250-254	21.860367978388933	30.048874612669263	26.740065605030477	21.35069180391133
255-259	20.33519857439588	29.362904781902998	27.17319501038557	23.12870163331555
260-264	19.107362972884008	31.094922987866475	27.227585893783413	22.570128145466104
265-269	20.657548551127658	30.819699669704775	26.611761234010196	21.91099054515737
270-274	20.090076387864205	29.953531661804934	27.888584951704253	22.067806998626608
275-279	19.894577937187155	31.16447794059226	25.945768021520266	22.995176100700316
280-284	19.94470108849869	29.947538676322033	27.799643598969386	22.308116636209892
285-289	19.95605143979206	31.623032132844514	26.184624814137997	22.23629161322543
290-294	18.877813468326845	32.3113628366798	25.74091688137748	23.06990681361588
295-299	20.127169335890947	31.36456193319183	24.859221592908302	23.64904713800892
300-301	16.235769497065935	32.20491924225055	34.52788213795217	17.03142912273135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.25
10	0.75
11	0.75
12	0.0
13	1.0
14	0.25
15	0.5
16	0.5
17	1.5
18	1.5
19	0.75
20	2.5
21	2.0
22	2.75
23	3.25
24	2.0
25	2.25
26	6.5
27	8.5
28	13.75
29	28.5
30	95.0
31	345.25
32	1042.75
33	2295.0
34	3932.75
35	5823.5
36	7463.75
37	8269.5
38	8907.0
39	10254.0
40	12290.25
41	13477.25
42	12800.25
43	11341.5
44	10380.0
45	8874.75
46	6883.0
47	4917.0
48	3308.0
49	2473.25
50	2227.75
51	2259.75
52	2151.75
53	1668.75
54	1004.5
55	552.5
56	325.0
57	235.25
58	198.0
59	189.75
60	174.5
61	178.5
62	163.5
63	118.0
64	72.0
65	48.5
66	32.0
67	21.5
68	16.75
69	16.75
70	10.25
71	10.5
72	9.75
73	7.5
74	4.5
75	3.0
76	2.5
77	1.5
78	1.25
79	1.25
80	1.25
81	2.25
82	1.75
83	1.0
84	0.5
85	1.0
86	2.75
87	1.75
88	0.75
89	1.5
90	1.75
91	0.5
92	0.5
93	0.75
94	0.25
95	0.75
96	1.5
97	0.75
98	0.5
99	0.0
100	1.6666666666666665
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	440515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.422994573448385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.9303882195326	10.441668287335714
2	6.068985322247934	2.1147979667172727
3	3.699555298187731	1.9337199565349117
4	3.1825009826090427	2.21794789399966
5	2.671514884897087	2.3272894671224265
6	2.434299463381506	2.544767180438657
7	2.4742937267999268	3.017672432260675
8	2.3443669340985176	3.2676713896776235
9	1.878526333475404	2.945659870279967
>10	14.981886415758417	43.83667144614684
>50	0.16204984313178	1.7927262445696626
>100	0.11198081068459714	3.694453445608386
>500	0.017232733691942625	2.226900109366623
>1k	0.04109343676889432	15.902402695553327
>5k	0.0013255947344804522	1.7356516143881668
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT	7515	1.7059577993938912	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	4927	1.1184636164489292	No Hit
GGGGGGAGCAGAAGCAGAGGATTGAAGCAGTACCGTAAGGAGTTGTGGAC	4606	1.0455943611454774	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	3242	0.7359567778622749	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCTACCT	3188	0.7236983984654325	No Hit
CCGGGTTATTAGTAGAAACAACAGCTTCAACAACAGCTGCTACAGCAACA	3101	0.7039487872149643	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA	3064	0.6955495272578686	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT	2914	0.661498473377751	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA	2716	0.6165510822559959	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	2586	0.5870401688932272	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT	2523	0.5727387262635778	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	2475	0.5618423890219403	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG	2387	0.5418657707456045	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	2355	0.5346015459178461	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	2281	0.5178030260036548	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT	2245	0.5096307730724267	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	2180	0.49487531639104226	No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1919	0.4356264826396377	No Hit
CCGGGTTATTAGTAGAAACACGAGCATAGTAGTTTGTATTGTAACGGGTA	1821	0.41337979410462755	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT	1760	0.39953236552671306	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	1729	0.392495147724822	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT	1636	0.3713834943191492	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	1629	0.369794445138077	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1486	0.3373324404390316	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1474	0.33460835612862216	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT	1442	0.32734413130086376	Illumina Single End PCR Primer 1 (97% over 34bp)
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCCACCT	1371	0.3112266324642748	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAACAAGA	1277	0.2898879720327344	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	1260	0.28602885259298777	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	1141	0.2590150165147611	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	1095	0.2485726933248584	No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA	1024	0.2324551944882694	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	1000	0.22700702586745058	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA	992	0.22519096966051097	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	985	0.22360192047943886	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	974	0.2211048431948969	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	889	0.2018092459961636	No Hit
CCGGGTTATTAGTAGAAACACGTGCATAGTAGTTTGTATTGTAACGGGTA	733	0.1663961499608413	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT	721	0.16367206565043188	No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	702	0.1593589321589503	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA	562	0.12757794853750723	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA	541	0.12281080099429077	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGATTGGTTTGGTGATGATAATG	526	0.119405695606279	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG	516	0.11713562534760452	No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	501	0.11373051995959275	No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA	481	0.10919037944224373	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	2.270070258674506E-4	0.0	0.0
7	2.270070258674506E-4	0.0	0.0
8	4.540140517349012E-4	0.0	0.0
9	6.810210776023518E-4	0.0	0.0
10-14	0.002633281500062427	0.0	0.0
15-19	0.0034505067931852483	0.0	0.0
20-24	0.0038591194397466604	0.0	0.0
25-29	0.004358534896655051	0.0	0.0
30-34	0.01080553443129065	0.0	0.0
35-39	0.01507326651759872	0.0	0.0
40-44	0.07096239628616505	0.0	0.0
45-49	0.17129950171957825	0.0	0.0
50-54	0.18174182490948093	0.0	0.0
55-59	0.19699669704777362	0.0	0.0
60-64	0.22727943429849154	0.0	0.0
65-69	0.3042348160675573	0.0	0.0
70-74	0.386275155216054	0.0	0.0
75-79	0.43903158802764947	0.0	0.0
80-84	0.5131266812707853	0.0	0.0
85-89	0.5903090700657185	0.0	0.0
90-94	0.6783423946971159	0.0	0.0
95-99	0.7927085343291375	0.0	0.0
100-104	0.9250990318150347	0.0	0.0
105-109	1.0762857110427568	0.0	0.0
110-114	1.2414106216587402	0.0	0.0
115-119	1.4083061870764901	0.0	0.0
120-124	1.603214419486283	0.0	0.0
125-129	1.814784967594747	0.0	1.3620421552047035E-4
130-134	2.0294428112550085	0.0	2.270070258674506E-4
135-139	2.2339761415615813	0.0	2.270070258674506E-4
140-144	2.5172809098441595	0.0	2.270070258674506E-4
145-149	2.9397863863886586	0.0	2.270070258674506E-4
150-154	3.278662474603589	0.0	2.270070258674506E-4
155-159	4.009080281034698	0.0	2.270070258674506E-4
160-164	4.264508586540753	0.0	2.270070258674506E-4
165-169	4.6479688546360505	0.0	2.270070258674506E-4
170-174	5.057398726490584	0.0	2.270070258674506E-4
175-179	5.3591364652736	0.0	2.270070258674506E-4
180-184	5.6685924429361085	0.0	2.270070258674506E-4
185-189	6.015141368625359	0.0	2.270070258674506E-4
190-194	6.367183864340602	0.0	2.270070258674506E-4
195-199	6.754548653280819	0.0	2.270070258674506E-4
200-204	7.150585110609174	0.0	2.270070258674506E-4
205-209	7.540219969808066	0.0	2.270070258674506E-4
210-214	7.946426341895281	0.0	2.270070258674506E-4
215-219	8.369794445138078	0.0	2.270070258674506E-4
220-224	8.802969251898347	0.0	2.270070258674506E-4
225-229	9.201729793537112	0.0	2.270070258674506E-4
230-234	9.605166679908743	0.0	2.270070258674506E-4
235-239	9.985267244021202	0.0	2.270070258674506E-4
240-244	10.336310908822629	0.0	2.270070258674506E-4
245-249	10.625563261182933	0.0	2.270070258674506E-4
250-254	10.904055480517123	0.0	2.270070258674506E-4
255-259	11.125977549005142	0.0	2.270070258674506E-4
260-264	11.324971907880549	0.0	2.270070258674506E-4
265-269	11.477747636289342	0.0	2.270070258674506E-4
270-274	11.60646061995619	0.0	2.270070258674506E-4
275-279	11.705481084639569	0.0	2.270070258674506E-4
280-284	11.77934917085684	0.0	2.270070258674506E-4
285-289	11.833331441608118	0.0	2.270070258674506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTT	7110	0.0	289.3987	1
CGGGTTA	7290	0.0	282.86008	2
TTATTAG	7955	0.0	258.84348	6
GGGGGGA	5340	0.0	253.56741	1
GTTATTA	8195	0.0	251.44293	5
GGGTTAT	8220	0.0	251.21655	3
TATTAGT	8365	0.0	246.86192	7
ATTAGTA	8460	0.0	246.00768	8
GGGGAGC	5635	0.0	241.33984	3
GGAGCAG	5680	0.0	237.61003	5
GGGAGCA	5720	0.0	237.49562	4
GAGCAGA	5735	0.0	235.3313	6
TTAGTAG	9000	0.0	230.75555	9
GGTTATT	9205	0.0	224.49483	4
CAGAAGC	6165	0.0	223.94162	9
GGGGGAG	6265	0.0	217.30647	2
GCAGAAG	6840	0.0	201.62646	8
AGCAGAA	7595	0.0	177.69916	7
GGGGTGC	120	7.239578E-10	86.041664	3
CCGGGTA	160	8.54925E-11	73.75	1
>>END_MODULE