FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_8033_flu.3520000003ba76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_8033_flu.3520000003ba76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440515
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT75151.7059577993938912No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG49271.1184636164489292No Hit
GGGGGGAGCAGAAGCAGAGGATTGAAGCAGTACCGTAAGGAGTTGTGGAC46061.0455943611454774No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT32420.7359567778622749No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCTACCT31880.7236983984654325No Hit
CCGGGTTATTAGTAGAAACAACAGCTTCAACAACAGCTGCTACAGCAACA31010.7039487872149643No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA30640.6955495272578686No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT29140.661498473377751No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA27160.6165510822559959No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT25860.5870401688932272No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT25230.5727387262635778No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT24750.5618423890219403No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG23870.5418657707456045No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA23550.5346015459178461No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC22810.5178030260036548No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT22450.5096307730724267No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG21800.49487531639104226No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA19190.4356264826396377No Hit
CCGGGTTATTAGTAGAAACACGAGCATAGTAGTTTGTATTGTAACGGGTA18210.41337979410462755No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT17600.39953236552671306No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC17290.392495147724822No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT16360.3713834943191492No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC16290.369794445138077No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA14860.3373324404390316No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA14740.33460835612862216No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT14420.32734413130086376Illumina Single End PCR Primer 1 (97% over 34bp)
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCCACCT13710.3112266324642748No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAACAAGA12770.2898879720327344No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC12600.28602885259298777No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG11410.2590150165147611No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT10950.2485726933248584No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA10240.2324551944882694No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA10000.22700702586745058No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA9920.22519096966051097No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA9850.22360192047943886No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG9740.2211048431948969No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT8890.2018092459961636No Hit
CCGGGTTATTAGTAGAAACACGTGCATAGTAGTTTGTATTGTAACGGGTA7330.1663961499608413No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT7210.16367206565043188No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7020.1593589321589503No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA5620.12757794853750723No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA5410.12281080099429077No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGATTGGTTTGGTGATGATAATG5260.119405695606279No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG5160.11713562534760452No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT5010.11373051995959275No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA4810.10919037944224373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT71100.0289.39871
CGGGTTA72900.0282.860082
TTATTAG79550.0258.843486
GGGGGGA53400.0253.567411
GTTATTA81950.0251.442935
GGGTTAT82200.0251.216553
TATTAGT83650.0246.861927
ATTAGTA84600.0246.007688
GGGGAGC56350.0241.339843
GGAGCAG56800.0237.610035
GGGAGCA57200.0237.495624
GAGCAGA57350.0235.33136
TTAGTAG90000.0230.755559
GGTTATT92050.0224.494834
CAGAAGC61650.0223.941629
GGGGGAG62650.0217.306472
GCAGAAG68400.0201.626468
AGCAGAA75950.0177.699167
GGGGTGC1207.239578E-1086.0416643
CCGGGTA1608.54925E-1173.751
Produced by FastQC (version 0.11.2)