##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_7965_flu.3520000003b95e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225577
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.263537506040066	34.0	34.0	34.0	34.0	34.0
2	33.46135022630853	34.0	34.0	34.0	34.0	34.0
3	33.4847834663995	34.0	34.0	34.0	34.0	34.0
4	33.480518847222896	34.0	34.0	34.0	34.0	34.0
5	33.426333358454094	34.0	34.0	34.0	34.0	34.0
6	36.93903633792452	38.0	38.0	38.0	36.0	38.0
7	36.05528932470952	38.0	38.0	38.0	34.0	38.0
8	36.839447284075945	38.0	38.0	38.0	35.0	38.0
9	36.92449141534819	38.0	38.0	38.0	36.0	38.0
10-14	36.932253731541785	38.0	38.0	38.0	36.2	38.0
15-19	36.85287684471378	38.0	38.0	38.0	36.0	38.0
20-24	36.961702655855866	38.0	38.0	38.0	36.2	38.0
25-29	36.889518878254435	38.0	38.0	38.0	36.0	38.0
30-34	36.56033194873591	38.0	38.0	38.0	34.6	38.0
35-39	36.830480944422526	38.0	38.0	38.0	35.8	38.0
40-44	36.37161146748117	38.0	37.4	38.0	33.8	38.0
45-49	36.68518332986076	38.0	38.0	38.0	35.0	38.0
50-54	36.65280680211192	38.0	38.0	38.0	34.6	38.0
55-59	36.31267726762924	38.0	37.4	38.0	33.6	38.0
60-64	36.50774768704257	38.0	37.2	38.0	34.0	38.0
65-69	36.36969726523538	38.0	37.0	38.0	33.8	38.0
70-74	36.2441578707049	38.0	37.0	38.0	33.8	38.0
75-79	36.28024310989152	38.0	37.0	38.0	34.0	38.0
80-84	35.60745111425367	38.0	36.8	38.0	29.8	38.0
85-89	35.81625520332303	38.0	37.0	38.0	31.6	38.0
90-94	35.8185497634954	38.0	37.0	38.0	31.6	38.0
95-99	34.91581854533042	38.0	36.0	38.0	26.6	38.0
100-104	35.26473354996298	38.0	36.0	38.0	28.2	38.0
105-109	35.09397855277799	38.0	35.8	38.0	27.2	38.0
110-114	34.6292121980521	38.0	35.2	38.0	24.0	38.0
115-119	34.62563913874198	38.0	35.0	38.0	24.4	38.0
120-124	34.19264641341981	37.6	34.6	38.0	23.4	38.0
125-129	33.81922359105759	37.0	34.0	38.0	23.0	38.0
130-134	33.63972568125296	37.0	34.0	38.0	23.0	38.0
135-139	33.001456708795665	37.0	31.4	38.0	21.2	38.0
140-144	31.747293385407197	36.2	28.2	38.0	17.4	38.0
145-149	31.652539930932676	35.2	28.0	37.6	18.0	38.0
150-154	30.64603128865088	35.0	26.0	37.0	14.2	38.0
155-159	30.516578374568326	35.0	26.0	37.0	15.0	37.6
160-164	29.79817977896683	35.0	24.4	37.0	11.0	37.4
165-169	28.70246168714009	33.2	23.0	36.6	10.0	37.0
170-174	28.079462001888494	32.6	21.8	36.2	9.4	37.0
175-179	27.283921676412046	30.6	20.2	36.0	9.2	37.0
180-184	26.42684227558661	28.8	20.0	35.0	9.0	37.0
185-189	25.71956360799195	27.8	19.8	35.0	9.0	36.8
190-194	24.7408636518794	25.8	17.0	34.8	8.6	36.0
195-199	23.92216937010422	25.0	15.4	34.2	8.0	36.0
200-204	23.069656037627947	24.2	15.0	32.4	8.0	36.0
205-209	22.206146903274714	23.0	12.6	31.4	8.0	35.8
210-214	20.92604565181734	21.0	9.6	29.4	8.0	35.0
215-219	20.420844323667747	20.4	9.0	28.6	8.0	34.6
220-224	19.74757444242986	20.0	9.0	27.8	8.0	34.0
225-229	18.952011064957865	18.6	9.0	26.4	8.0	33.2
230-234	18.053992206652275	17.0	9.0	25.0	8.0	31.8
235-239	17.05472809727942	15.2	9.0	23.8	8.0	30.8
240-244	16.19978543911835	14.2	8.0	22.8	7.0	29.0
245-249	15.518859635512484	13.6	8.0	21.2	7.0	28.2
250-254	14.798018414997983	12.8	8.0	20.2	7.0	26.8
255-259	14.18032157533791	12.0	8.0	19.2	7.0	25.4
260-264	13.563066270054128	11.4	8.0	18.0	7.0	24.4
265-269	13.137017515083542	10.6	8.0	17.2	7.0	23.6
270-274	12.640604316929473	9.4	8.0	16.0	7.0	22.4
275-279	12.106065777982684	9.0	7.2	14.8	7.0	21.2
280-284	11.937075145072415	8.6	7.2	14.4	7.0	21.0
285-289	11.578834721624988	8.0	7.6	13.6	7.0	20.4
290-294	11.273964100950007	8.0	7.0	13.0	7.0	19.2
295-299	10.94657256723868	8.0	7.0	13.0	7.0	19.0
300-301	10.59334949928406	8.0	7.0	12.5	7.0	17.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.15006564575901393
1101	2	0.06032927680873712
1101	3	0.09913781285320766
1101	4	0.12769704599387666
1101	5	0.0865265271331026
1101	6	0.19366787933638108
1101	7	0.9151805264407429
1101	8	0.3738002783097727
1101	9	0.17441973041075443
1101	10-14	0.21559194469499943
1101	15-19	0.35090794317927276
1101	20-24	0.246475038324121
1101	25-29	0.19005113788986705
1101	30-34	0.5490934379961914
1101	35-39	0.3140174013233761
1101	40-44	0.6617609414770911
1101	45-49	0.2445024744745652
1101	50-54	0.2784802558751167
1101	55-59	0.3283328606467535
1101	60-64	0.31757524201310616
1101	65-69	0.28459223743253403
1101	70-74	0.36290847223380496
1101	75-79	0.34944247615912616
1101	80-84	0.8060575471026823
1101	85-89	0.2581469451336673
1101	90-94	0.3160946340297883
1101	95-99	1.110523429765344
1101	100-104	0.43577705336513617
1101	105-109	0.6573750879744722
1101	110-114	0.8347170758914189
1101	115-119	0.8025972402845127
1101	120-124	0.8385200062524021
1101	125-129	0.6869425293051279
1101	130-134	0.7793707358527939
1101	135-139	0.6592736318783707
1101	140-144	1.4758357398794963
1101	145-149	1.558248123271813
1101	150-154	1.4003038123472074
1101	155-159	0.6291785833986552
1101	160-164	0.7598601559640947
1101	165-169	0.7299320731190271
1101	170-174	0.7544667279208639
1101	175-179	0.9770983535462179
1101	180-184	0.8017624364019618
1101	185-189	0.33107531042310967
1101	190-194	0.5386769327583956
1101	195-199	0.8559186976230997
1101	200-204	1.2743905445065131
1101	205-209	0.38123985158845386
1101	210-214	0.8205423401435326
1101	215-219	0.672110516777515
1101	220-224	0.5293634607914797
1101	225-229	0.32648502594463835
1101	230-234	0.915918611065262
1101	235-239	0.5452192350354323
1101	240-244	0.20114621289916101
1101	245-249	0.09033364593578952
1101	250-254	-0.0019764306013989597
1101	255-259	0.1147872874443916
1101	260-264	0.20614378955904833
1101	265-269	0.2243343676926397
1101	270-274	0.10980037855187241
1101	275-279	-0.05394668645676859
1101	280-284	-0.13629616728013794
1101	285-289	0.2911704061325455
1101	290-294	0.12771661331111517
1101	295-299	-0.027152424750402915
1101	300-301	0.023221094233472073
1102	1	-0.3922820682241337
1102	2	-0.032141601224324745
1102	3	-0.05444603078600352
1102	4	-0.06336550292642329
1102	5	-0.024367160421569167
1102	6	-0.1470389902248357
1102	7	0.5692276740994728
1102	8	-0.07231257218979437
1102	9	0.08722073414021025
1102	10-14	-0.13904109550021815
1102	15-19	-0.030230691196166504
1102	20-24	-0.0339303670812825
1102	25-29	-0.22923209338228645
1102	30-34	0.12425804041803445
1102	35-39	-0.011535685835333709
1102	40-44	0.317420732443523
1102	45-49	-0.026852675394216874
1102	50-54	0.029711320841279587
1102	55-59	-0.061285100585351415
1102	60-64	-0.0368684478437018
1102	65-69	0.032268810939598325
1102	70-74	-0.06447078095465031
1102	75-79	0.1848521405083119
1102	80-84	0.5904767192560954
1102	85-89	0.18320680055978755
1102	90-94	0.17313800619240283
1102	95-99	0.5682994553650502
1102	100-104	0.4422857554232422
1102	105-109	0.775853148658662
1102	110-114	0.42679544045699913
1102	115-119	0.5433564833435796
1102	120-124	0.7245506706476377
1102	125-129	0.5742261289183759
1102	130-134	0.7562510019792299
1102	135-139	0.7538365721610703
1102	140-144	1.5753471124104514
1102	145-149	1.444945830348562
1102	150-154	1.3391804871480737
1102	155-159	1.0723878701982272
1102	160-164	1.2968818880880448
1102	165-169	1.0285122333473957
1102	170-174	1.583930561430698
1102	175-179	1.2880271486104462
1102	180-184	0.9746116697920755
1102	185-189	1.1617149577365211
1102	190-194	1.4676245132178956
1102	195-199	1.4593091621932857
1102	200-204	1.7412243075711054
1102	205-209	1.0959326321032101
1102	210-214	1.6482013023425246
1102	215-219	1.8054701327819345
1102	220-224	1.5530305264943216
1102	225-229	1.5584639384175887
1102	230-234	0.7314677845999533
1102	235-239	0.6718669858526916
1102	240-244	0.639428362169383
1102	245-249	0.5258584168736782
1102	250-254	1.345498130842497
1102	255-259	0.7465538282611899
1102	260-264	0.6188843623294336
1102	265-269	0.5750451508778696
1102	270-274	0.4804551048284065
1102	275-279	0.6730394777312689
1102	280-284	0.5118477389770568
1102	285-289	0.3843814195309516
1102	290-294	-0.040568643837005425
1102	295-299	-0.007879553071228784
1102	300-301	0.03491160714843211
1103	1	0.029720471228067424
1103	2	0.13090483447312096
1103	3	0.2262201219439106
1103	4	0.22904561624644515
1103	5	0.17751928978702836
1103	6	0.22511546288599504
1103	7	1.0953024065500472
1103	8	0.4124280409065264
1103	9	0.4258974981525441
1103	10-14	0.12811381108421926
1103	15-19	0.20516745863152153
1103	20-24	0.21526698463284788
1103	25-29	0.05389642103816783
1103	30-34	0.4982467942725677
1103	35-39	0.24168624414834738
1103	40-44	0.4904707959298378
1103	45-49	0.15464333403803465
1103	50-54	0.18517794518373876
1103	55-59	0.19132464002775862
1103	60-64	0.3527175307523436
1103	65-69	0.36090793664248366
1103	70-74	0.335365968546526
1103	75-79	0.2949668693310912
1103	80-84	0.8673949126172431
1103	85-89	0.5171130222176856
1103	90-94	0.46284869003060436
1103	95-99	1.1340236527167633
1103	100-104	0.4898301958368094
1103	105-109	0.7184660635870159
1103	110-114	0.509681346497274
1103	115-119	0.6439278600894554
1103	120-124	1.055087583399306
1103	125-129	0.9279650188149802
1103	130-134	0.8515915606587114
1103	135-139	0.9592170365180479
1103	140-144	1.487579562981832
1103	145-149	1.5905720774009815
1103	150-154	2.028335665816666
1103	155-159	0.8363661941597726
1103	160-164	0.8547768636004704
1103	165-169	1.806185460265592
1103	170-174	1.9498973671626025
1103	175-179	1.7152287629764906
1103	180-184	1.4270445556981173
1103	185-189	0.6426350556868172
1103	190-194	1.276741600103442
1103	195-199	1.410839635619734
1103	200-204	1.311669165180394
1103	205-209	0.814690567891347
1103	210-214	1.796437290008047
1103	215-219	1.6665870382780348
1103	220-224	1.5415440400078353
1103	225-229	0.6378218197888899
1103	230-234	1.2421085428078307
1103	235-239	0.7343397774362188
1103	240-244	0.47851778716484716
1103	245-249	0.5545202162569574
1103	250-254	0.49055526851708464
1103	255-259	0.3521202167973758
1103	260-264	0.11690948476730689
1103	265-269	0.41251794246139717
1103	270-274	-0.01877399003827307
1103	275-279	0.01725542186332163
1103	280-284	0.3046805886052084
1103	285-289	0.14359600324218036
1103	290-294	0.15421867704091952
1103	295-299	-0.18557998840589107
1103	300-301	-0.20923291489038576
2116	1	0.0445540215970297
2116	2	0.00431463112742847
2116	3	0.07699978069712188
2116	4	0.11948992713050188
2116	5	-0.005475134066855958
2116	6	0.21317850338818545
2116	7	0.7503827097321221
2116	8	0.19052302460102766
2116	9	0.20926255207755418
2116	10-14	0.09722240680805783
2116	15-19	0.1520538321331486
2116	20-24	0.09125115772710757
2116	25-29	0.12416365311046462
2116	30-34	0.5149173564504892
2116	35-39	0.012976326176691089
2116	40-44	0.28305763800581474
2116	45-49	0.09150878307917765
2116	50-54	0.20592989015685959
2116	55-59	0.4652942773000035
2116	60-64	0.36252030658237544
2116	65-69	0.4766958872696634
2116	70-74	0.41825298759001583
2116	75-79	0.3369716107247953
2116	80-84	0.8381034504649207
2116	85-89	0.5003250382004154
2116	90-94	0.5538773276033666
2116	95-99	1.242879819311085
2116	100-104	0.618281340560614
2116	105-109	0.38487896552030776
2116	110-114	0.6335118583674486
2116	115-119	0.9250090314140422
2116	120-124	1.144617005639347
2116	125-129	0.883808285702024
2116	130-134	0.8226907626354034
2116	135-139	1.2800697082822694
2116	140-144	1.8986455974626644
2116	145-149	1.803289808655233
2116	150-154	2.2350765697978794
2116	155-159	1.8305312747968578
2116	160-164	1.8862514815621125
2116	165-169	1.8127683177900522
2116	170-174	1.602453935551086
2116	175-179	2.0106365117048846
2116	180-184	2.1612410230259087
2116	185-189	1.505542553989489
2116	190-194	1.5662031291472616
2116	195-199	1.0763215103623551
2116	200-204	1.0077402638258697
2116	205-209	0.5416942519319967
2116	210-214	1.327071590541177
2116	215-219	2.1769802623144514
2116	220-224	1.2839155159614037
2116	225-229	1.0430701864566743
2116	230-234	1.3995278222495422
2116	235-239	0.9925849052646392
2116	240-244	0.8336531840061454
2116	245-249	0.6812094768889203
2116	250-254	1.2195423242896766
2116	255-259	1.5197268146804443
2116	260-264	0.6358216354234241
2116	265-269	0.6186071830593054
2116	270-274	0.5677909481343999
2116	275-279	0.725228420405287
2116	280-284	0.3570434944340075
2116	285-289	-0.1163603670317297
2116	290-294	0.39451662800200005
2116	295-299	0.390874985048983
2116	300-301	0.12794167079416674
1117	1	0.0677956565594755
1117	2	-0.12711157119429117
1117	3	0.16513752335637832
1117	4	0.03857099239242956
1117	5	0.073053789940559
1117	6	-0.09471828716883834
1117	7	0.5965196719797063
1117	8	0.034757733409925606
1117	9	0.0043069379911173655
1117	10-14	-0.03459400134724433
1117	15-19	-0.05590636687183803
1117	20-24	0.03242275074241974
1117	25-29	0.052341391867663845
1117	30-34	0.34106602487307214
1117	35-39	0.07637501898714305
1117	40-44	0.488154701706911
1117	45-49	0.11572593262557263
1117	50-54	0.040970374012097466
1117	55-59	0.16906951873986742
1117	60-64	0.25719399695403666
1117	65-69	0.24165540341442693
1117	70-74	0.23800228034193793
1117	75-79	0.33148921876303916
1117	80-84	0.7669738045767147
1117	85-89	0.4179135661489042
1117	90-94	0.25081421180978936
1117	95-99	0.8432310045613107
1117	100-104	0.4159498607049983
1117	105-109	0.28563498932285825
1117	110-114	0.8411745254243215
1117	115-119	0.7641125335113941
1117	120-124	1.0882761608437121
1117	125-129	0.75583052743454
1117	130-134	0.8900988203858731
1117	135-139	1.068597656775097
1117	140-144	1.4424266919900326
1117	145-149	1.8275703666495708
1117	150-154	2.202387076089959
1117	155-159	1.9799947417756947
1117	160-164	1.7686580760407011
1117	165-169	1.5142413448118077
1117	170-174	1.4463179488180806
1117	175-179	1.3279957937261493
1117	180-184	1.6465887939750843
1117	185-189	1.3665219706317693
1117	190-194	-0.5997465343002091
1117	195-199	1.403362774629251
1117	200-204	1.5852205096555423
1117	205-209	1.9192964370846646
1117	210-214	2.3729451638510923
1117	215-219	2.1220197706551005
1117	220-224	1.5529819485045664
1117	225-229	1.615794798689624
1117	230-234	1.4296858556121421
1117	235-239	1.101227964478376
1117	240-244	1.0941448433564531
1117	245-249	1.253656067465453
1117	250-254	1.1465933437024152
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2108	260-264	-0.8636562228101621
2108	265-269	-0.872036955981466
2108	270-274	-0.7558351458182813
2108	275-279	-0.7156825363620314
2108	280-284	-1.035925653227027
2108	285-289	-1.3071670596412908
2108	290-294	-1.0941555227951696
2108	295-299	-0.7987757710714067
2108	300-301	2.1961567012524377
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	47.0
11	249.0
12	468.0
13	413.0
14	442.0
15	697.0
16	1065.0
17	1559.0
18	2140.0
19	2348.0
20	3039.0
21	4064.0
22	5384.0
23	7614.0
24	10590.0
25	15003.0
26	20878.0
27	29065.0
28	36197.0
29	37039.0
30	28786.0
31	14060.0
32	3954.0
33	449.0
34	22.0
35	2.0
36	2.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.897006343731853	23.544066992645526	14.072799975174775	33.48612668844785
2	44.93188578622821	23.757741259082266	17.5505481498557	13.759824804833826
3	43.67909848965098	22.602924943589105	19.12030038523431	14.597676181525598
4	43.95217597538756	21.833786245938196	19.10921769506643	15.104820083607814
5	28.187714172987494	21.788125562446524	35.445989617735854	14.578170646830129
6	27.230169742482612	21.400231406570704	35.79442939661402	15.575169454332666
7	16.602756486698556	47.681722870682734	20.394366446933862	15.321154195684844
8	26.540826414040435	20.428501132650936	36.79807781821729	16.232594635091342
9	15.49315754709035	19.31358250176215	37.92053267842023	27.27272727272727
10-14	26.183786467591997	34.229464883387934	23.575807817286336	16.01094083173373
15-19	21.362904906085284	35.1750400085115	21.884500636146413	21.577554449256795
20-24	25.229611174898146	30.90802697083479	26.435851172770274	17.426510681496783
25-29	19.181565496482353	25.79004065130753	37.830807218821064	17.197586633389044
30-34	19.276521985840755	28.41530829827509	31.541424879309503	20.766744836574652
35-39	20.525585498521572	30.030721217145366	27.801681909059877	21.64201137527319
40-44	19.872948039915418	29.119724085345585	29.153326801934593	21.854001072804408
45-49	20.999215345536115	30.316920608040714	26.38540276712608	22.29846127929709
50-54	21.822880878813	29.729804013707074	27.663369935764727	20.783945171715203
55-59	21.569308927771893	26.984754651405062	29.936917327564423	21.509019093258622
60-64	21.75523213802826	26.02924943589107	28.941603088967405	23.27391533711327
65-69	20.01542710471369	26.880400040784302	29.288801606546766	23.815371247955245
70-74	20.661503610740457	26.03802692650403	29.537585835435348	23.76288362732016
75-79	20.077933477260537	26.029958728061814	30.776807919247084	23.115299875430562
80-84	22.955531813970396	25.857246084485563	27.425047766394623	23.762174335149417
85-89	20.72622652132088	28.194895756216283	26.30853322812166	24.770344494341177
90-94	19.788719594639524	30.09854728097279	29.161306338855468	20.951426785532213
95-99	22.024585839868426	29.963959091574054	26.59180678881269	21.419648279744834
100-104	22.368415219636752	28.43596643274802	26.690043754460785	22.505574593154442
105-109	23.295016779192913	28.064563319842005	23.81838573968091	24.822034161284172
110-114	21.433213492510315	28.28275932386724	28.966073668858083	21.31795351476436
115-119	23.447514595902952	27.874384356561173	26.950442642645307	21.72765840489057
120-124	18.712812033141677	28.74211466594555	27.920311024616872	24.6247622762959
125-129	20.432313578068687	24.67077760587294	31.27136188529859	23.625546930759786
130-134	24.999179880927578	28.709841872176682	25.989529074329386	20.30144917256635
135-139	24.026297007230347	27.627816665706167	24.773358986066842	23.572527340996643
140-144	20.84414634470713	29.24872659889971	26.772587630831158	23.134539425562004
145-149	20.98228099495959	27.026159581872268	29.174428244014237	22.817131179153904
150-154	23.224796854289224	25.45676199257903	25.682937533525134	25.63550361960661
155-159	21.52205233689605	26.6445603940118	30.060334165273943	21.773053103818206
160-164	24.98384143773523	28.91899440102493	26.140874291261962	19.956289869977876
165-169	20.491628135847183	25.676199257903065	28.47542080974567	25.356751796504078
170-174	22.217956617917604	28.89266192918604	26.172349131338745	22.717032321557607
175-179	20.166772321646267	28.807990176303434	28.864467565398954	22.16076993665134
180-184	20.03785846961348	29.507706902742743	26.669562943030538	23.784871684613236
185-189	22.176197041365032	27.445528577824867	27.746889088869874	22.631385291940223
190-194	20.307566817539023	27.62710737353542	27.131489469227805	24.933836339697756
195-199	21.160579314380456	29.68760112954778	27.36236407080509	21.78945548526667
200-204	22.569942857649494	27.314752833843876	27.766217300522662	22.349087007983968
205-209	21.36654002846035	27.34729161217677	28.353067910292275	22.933100449070608
210-214	23.71952814338341	26.56742487044335	27.997978517313378	21.715068468859858
215-219	23.288633149656217	26.860007890875398	28.13983695146225	21.711522008006135
220-224	19.658653142829277	26.91329346520257	28.707713995664452	24.7203393963037
225-229	22.03779640654854	28.380464320387272	26.56769085500738	23.014048418056806
230-234	21.627382224251587	26.872686488427455	28.603004738958315	22.896926548362643
235-239	22.21839992552432	26.959663440864983	26.078633903279147	24.74330273033155
240-244	23.260882093475843	31.016991980565393	23.42047283189332	22.30165309406544
245-249	21.489868204648523	29.217163097301587	24.680885019306047	24.612083678743844
250-254	22.074856922469934	27.18176055182931	25.719022772711757	25.024359752989
255-259	22.140732432827814	27.637214786968528	27.3444544434938	22.87759833670986
260-264	21.893278126759377	28.577913528418236	26.609273108517268	22.91953523630512
265-269	21.396152976588926	28.335335606023666	26.998231202649208	23.270280214738204
270-274	20.378762019177486	30.39458810073722	24.85342033983961	24.37322954024568
275-279	21.68634213594471	29.177886043346618	24.008476041440396	25.127295779268277
280-284	19.32528582257943	29.94853198686036	27.01348098432021	23.71270120624
285-289	21.598833214379127	28.50095532789247	27.326633477703844	22.57357798002456
290-294	20.98245831800228	30.756770415423556	25.099544723087902	23.16122654348626
295-299	20.452617066456245	31.868674554586683	22.95508850636368	24.723619872593396
300-301	16.232372981287984	33.35601590587693	30.197670861834318	20.213940251000768
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.25
27	0.0
28	0.75
29	1.5
30	1.25
31	2.5
32	2.0
33	9.75
34	27.5
35	95.5
36	320.5
37	903.25
38	1811.0
39	2765.5
40	3760.75
41	4975.5
42	6781.5
43	8578.0
44	9242.0
45	8329.75
46	6600.75
47	5248.25
48	4362.5
49	3555.75
50	2855.0
51	2177.75
52	1377.0
53	649.0
54	286.5
55	125.5
56	69.0
57	47.25
58	34.0
59	27.0
60	24.25
61	19.75
62	16.5
63	12.5
64	13.0
65	7.5
66	6.25
67	3.75
68	4.0
69	2.25
70	1.75
71	1.75
72	1.25
73	0.75
74	1.0
75	0.25
76	0.75
77	1.0
78	0.5
79	0.5
80	1.25
81	0.0
82	0.25
83	0.25
84	0.25
85	0.25
86	0.75
87	0.25
88	0.25
89	0.25
90	0.25
91	0.75
92	0.25
93	0.5
94	0.75
95	0.0
96	0.0
97	0.0
98	0.0
99	0.25
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	225577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.68067908564056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.83040299866828	9.478038567810376
2	11.309755671799552	4.6778685517097935
3	8.887652275526806	5.514080536047987
4	7.066720980480038	5.8457835514028345
5	5.731089924348203	5.926141576819662
6	4.408445370816988	5.47017863882663
7	3.775899047700727	5.466170952569216
8	2.7828458834336725	4.604091412807014
9	2.1297268303286114	3.9639777406294026
>10	7.982603618154298	24.7759883308197
>50	0.02915425337745392	0.4007601998278839
>100	0.017524016299946273	0.833887750605874
>500	0.015329722884448845	2.207428190307645
>1k	0.02627952494476939	11.77898899743971
>5k	0.0065698812361923474	9.056615002376263
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	8654	3.836384028513545	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	6142	2.7227953204449036	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5201	2.305642862525878	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4034	1.788302885489212	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3452	1.530297858380952	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	2721	1.2062399978721237	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2573	1.1406304720782703	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2565	1.1370840112245486	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	2083	0.9234097447878108	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2031	0.9003577492386191	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1584	0.7021992490369142	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1473	0.6529921046915245	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1318	0.5842794256506647	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	1122	0.49739113473448093	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1052	0.4663596022644153	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	973	0.43133830133391265	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	767	0.3400169343505765	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT	703	0.31164524752080225	Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	670	0.2970160964991998	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	642	0.2846034835111736	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	583	0.25844833471497536	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	536	0.23761287719935986	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	405	0.17953958071966555	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	334	0.1480647406428847	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	291	0.1290025135541301	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	7.092921707443578E-4	0.0	0.0
15-19	0.0010639382561165368	0.0	0.0
20-24	0.002216538033576118	0.0	0.0
25-29	0.002482522597605252	0.0	0.0
30-34	0.004521737588495281	0.0	0.0
35-39	0.006738275622071399	0.0	0.0
40-44	0.008511506048932294	0.0	0.0
45-49	0.010196074954450143	0.0	0.0
50-54	0.013033243637427574	0.0	0.0
55-59	0.01728899666189372	0.0	0.0
60-64	0.024736564454709477	0.0	0.0
65-69	0.03732650048542183	0.0	0.0
70-74	0.05488148171134468	0.0	0.0
75-79	0.0763375698763615	0.0	0.0
80-84	0.11020627102940458	0.0	0.0
85-89	0.1512565554112343	0.0	0.0
90-94	0.20480811430243334	0.0	0.0
95-99	0.2743187470353804	0.0	0.0
100-104	0.3596997920887325	0.0	0.0
105-109	0.47389583157857407	0.0	0.0
110-114	0.606444805986426	0.0	0.0
115-119	0.7477712710072393	0.0	0.0
120-124	0.9161395000376812	0.0	0.0
125-129	1.0944378194585442	0.0	0.0
130-134	1.2936602579163654	0.0	0.0
135-139	1.5138068154111457	0.0	0.0
140-144	1.7703932581779172	0.0	0.0
145-149	2.0669660470704017	0.0	0.0
150-154	2.3802072019753786	0.0	0.0
155-159	2.74221219361903	0.0	0.0
160-164	3.1509418070104664	0.0	0.0
165-169	3.560026066487275	0.0	0.0
170-174	4.013618409678292	0.0	0.0
175-179	4.497444331647286	0.0	0.0
180-184	4.998559250278175	0.0	0.0
185-189	5.54817202108371	0.0	0.0
190-194	6.146105321021204	0.0	0.0
195-199	6.7012151061500065	0.0	0.0
200-204	7.253221738031803	0.0	1.7732304268608945E-4
205-209	7.784126927833955	0.0	4.433076067152236E-4
210-214	8.322745669992951	0.0	4.433076067152236E-4
215-219	8.922629523399994	0.0	4.433076067152236E-4
220-224	9.496624212574863	0.0	4.433076067152236E-4
225-229	10.036306892989975	0.0	4.433076067152236E-4
230-234	10.536269211843406	0.0	4.433076067152236E-4
235-239	11.008303151473777	0.0	4.433076067152236E-4
240-244	11.440971375627834	0.0	4.433076067152236E-4
245-249	11.798631952725678	0.0	4.433076067152236E-4
250-254	12.13563439535059	0.0	4.433076067152236E-4
255-259	12.392309499638705	0.0	4.433076067152236E-4
260-264	12.624691347078826	0.0	4.433076067152236E-4
265-269	12.819392047948151	0.0	4.433076067152236E-4
270-274	12.97419506421311	0.0	4.433076067152236E-4
275-279	13.090696303257868	0.0	4.433076067152236E-4
280-284	13.170403010945265	0.0	4.433076067152236E-4
285-289	13.231047491543908	0.0	8.866152134304472E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	3680	0.0	290.59103	1
GGGTTAT	3725	0.0	287.87247	2
GTTATTA	3735	0.0	286.70682	4
GGTTATT	3815	0.0	280.69464	3
TTATTAG	3845	0.0	276.20285	5
TTAGTAG	3840	0.0	276.17838	8
GGGGAGC	2505	0.0	272.03595	3
GGGGGGA	2515	0.0	270.95428	1
GGAGCGA	1970	0.0	269.54315	5
TATTAGT	3940	0.0	269.1688	6
TAGTAGA	3935	0.0	269.13596	9
ATTAGTA	3935	0.0	268.3863	7
GGGAGCG	2005	0.0	265.57355	4
CGAAAGC	2100	0.0	256.36905	9
GCGAAAG	2145	0.0	251.67831	8
GAGCGAA	2140	0.0	248.82008	6
AGCGAAA	2190	0.0	243.81279	7
GGGGGAG	2955	0.0	230.60913	2
GGGAGCA	815	0.0	184.60123	4
GGCGCGA	20	0.006734131	147.5	5
>>END_MODULE