FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_7965_flu.3520000003b95e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_7965_flu.3520000003b95e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225577
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC86543.836384028513545No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT61422.7227953204449036No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG52012.305642862525878No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA40341.788302885489212No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA34521.530297858380952No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA27211.2062399978721237No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT25731.1406304720782703No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT25651.1370840112245486No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG20830.9234097447878108No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT20310.9003577492386191No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG15840.7021992490369142No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14730.6529921046915245No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13180.5842794256506647No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA11220.49739113473448093No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA10520.4663596022644153No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG9730.43133830133391265No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7670.3400169343505765No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT7030.31164524752080225Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6700.2970160964991998No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6420.2846034835111736No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT5830.25844833471497536No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA5360.23761287719935986No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4050.17953958071966555No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA3340.1480647406428847No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA2910.1290025135541301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA36800.0290.591031
GGGTTAT37250.0287.872472
GTTATTA37350.0286.706824
GGTTATT38150.0280.694643
TTATTAG38450.0276.202855
TTAGTAG38400.0276.178388
GGGGAGC25050.0272.035953
GGGGGGA25150.0270.954281
GGAGCGA19700.0269.543155
TATTAGT39400.0269.16886
TAGTAGA39350.0269.135969
ATTAGTA39350.0268.38637
GGGAGCG20050.0265.573554
CGAAAGC21000.0256.369059
GCGAAAG21450.0251.678318
GAGCGAA21400.0248.820086
AGCGAAA21900.0243.812797
GGGGGAG29550.0230.609132
GGGAGCA8150.0184.601234
GGCGCGA200.006734131147.55
Produced by FastQC (version 0.11.2)