##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_7765_flu.3520000003b9ba.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	311929
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.2628418646551	34.0	34.0	34.0	34.0	34.0
2	33.44593481208865	34.0	34.0	34.0	34.0	34.0
3	33.47113285395074	34.0	34.0	34.0	34.0	34.0
4	33.4706263284273	34.0	34.0	34.0	34.0	34.0
5	33.421137502444466	34.0	34.0	34.0	34.0	34.0
6	36.95509234473229	38.0	38.0	38.0	36.0	38.0
7	36.02297958830375	38.0	38.0	38.0	34.0	38.0
8	36.83178543835296	38.0	38.0	38.0	35.0	38.0
9	36.91394836645519	38.0	38.0	38.0	36.0	38.0
10-14	36.91763959106079	38.0	38.0	38.0	36.2	38.0
15-19	36.8304139724104	38.0	38.0	38.0	36.0	38.0
20-24	36.93262697601056	38.0	38.0	38.0	36.0	38.0
25-29	36.862402020972716	38.0	38.0	38.0	36.0	38.0
30-34	36.52003885499585	38.0	38.0	38.0	34.6	38.0
35-39	36.797378890709105	38.0	38.0	38.0	35.8	38.0
40-44	36.32322804227885	38.0	37.2	38.0	33.6	38.0
45-49	36.663452901140964	38.0	38.0	38.0	35.0	38.0
50-54	36.5786393698566	38.0	37.8	38.0	34.6	38.0
55-59	36.23541895751918	38.0	37.0	38.0	33.4	38.0
60-64	36.40982403046847	38.0	37.0	38.0	34.0	38.0
65-69	36.33943365317108	38.0	37.0	38.0	34.0	38.0
70-74	36.220705993992226	38.0	37.0	38.0	33.6	38.0
75-79	36.23486819115889	38.0	37.0	38.0	33.6	38.0
80-84	35.5205126807703	38.0	36.8	38.0	29.2	38.0
85-89	35.744495702547695	38.0	37.0	38.0	31.2	38.0
90-94	35.72399103642175	38.0	37.0	38.0	31.0	38.0
95-99	34.78767668283488	38.0	35.6	38.0	25.6	38.0
100-104	35.2040496395013	38.0	36.0	38.0	27.8	38.0
105-109	34.94279146857137	38.0	35.8	38.0	26.4	38.0
110-114	34.36346732750081	38.0	35.0	38.0	24.0	38.0
115-119	34.4372892549266	38.0	35.0	38.0	24.0	38.0
120-124	34.01660441959549	37.4	34.6	38.0	23.0	38.0
125-129	33.72675320345335	37.0	34.0	38.0	23.0	38.0
130-134	33.52551958939374	37.0	33.2	38.0	22.6	38.0
135-139	32.90232200276345	37.0	31.2	38.0	21.0	38.0
140-144	31.57839636583966	36.0	27.6	38.0	16.8	38.0
145-149	31.442778965726177	35.2	28.0	37.6	17.2	38.0
150-154	30.458041413270326	35.0	25.4	37.0	12.0	38.0
155-159	30.118419896835498	35.0	24.8	37.0	11.4	37.4
160-164	29.512787845952126	34.2	23.6	37.0	10.2	37.4
165-169	28.209513062267376	32.6	21.6	36.6	9.8	37.0
170-174	27.569889301732122	31.2	20.4	36.2	9.2	37.0
175-179	26.97914653655159	29.8	20.0	35.8	9.2	37.0
180-184	25.833605083208038	27.6	19.2	35.0	9.0	37.0
185-189	25.129603210987113	26.4	17.6	35.0	8.6	36.4
190-194	24.075469738305834	25.2	15.6	34.6	8.0	36.0
195-199	23.189140477480453	24.2	15.0	33.6	8.0	36.0
200-204	22.24710366782184	23.0	12.4	31.4	8.0	35.8
205-209	21.37784559947937	22.0	9.4	30.4	8.0	35.0
210-214	19.987924816224204	20.2	9.0	28.2	8.0	34.8
215-219	19.490074343841066	19.4	9.0	27.6	8.0	34.0
220-224	18.735353878606993	17.8	9.0	26.0	8.0	33.4
225-229	17.83977700053538	16.2	9.0	25.2	8.0	32.2
230-234	17.031553334252347	15.2	9.0	23.8	7.6	30.8
235-239	16.19239955246226	14.4	8.6	22.8	7.0	29.0
240-244	15.29763952694363	13.0	8.0	20.8	7.0	28.0
245-249	14.536189966306434	12.2	8.0	20.0	7.0	26.6
250-254	13.960769918795624	11.6	8.0	18.6	7.0	25.2
255-259	13.325192591903924	10.6	8.0	17.2	7.0	24.2
260-264	12.850231302636178	9.8	7.8	16.2	7.0	23.0
265-269	12.36343398658028	9.0	7.0	15.2	7.0	21.8
270-274	11.889197862334056	8.4	7.0	14.0	7.0	21.0
275-279	11.55490448146854	8.0	7.0	13.8	7.0	20.4
280-284	11.3714717131142	8.0	7.0	13.2	7.0	19.6
285-289	11.07342247755098	8.0	7.0	13.0	7.0	19.0
290-294	10.7718064046626	8.0	7.0	13.0	7.0	18.6
295-299	10.58833580718689	8.0	7.0	13.0	7.0	18.0
300-301	10.317463268884907	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.04837927444885537
1101	2	-0.030592242830500993
1101	3	0.08027837356219436
1101	4	0.07496443823630727
1101	5	0.07768024636170878
1101	6	0.06458734821880796
1101	7	1.070728758181069
1101	8	0.27667156698284145
1101	9	-0.02950585106821535
1101	10-14	0.047866905933993564
1101	15-19	0.17893628166811482
1101	20-24	0.03932796052328058
1101	25-29	0.06598570042633156
1101	30-34	0.2633037810141019
1101	35-39	0.10773659500579669
1101	40-44	0.4696265106356421
1101	45-49	0.08952458414292153
1101	50-54	0.15682904468503267
1101	55-59	0.2360325063584483
1101	60-64	0.13272815189856146
1101	65-69	0.16582045673838053
1101	70-74	0.14302186789796423
1101	75-79	0.03848125946245062
1101	80-84	0.6133146174787854
1101	85-89	0.12323410360007614
1101	90-94	0.3121872574098603
1101	95-99	0.8082586340309703
1101	100-104	0.18431724921943982
1101	105-109	0.2597365316577793
1101	110-114	0.4474771342733703
1101	115-119	0.4725201286158125
1101	120-124	0.9322475522713063
1101	125-129	0.4900236452919984
1101	130-134	0.6238719141560907
1101	135-139	0.3760668462641519
1101	140-144	0.8748614451044787
1101	145-149	1.087699354868274
1101	150-154	0.6821259833125843
1101	155-159	0.2528777727087643
1101	160-164	0.7684823464378887
1101	165-169	0.42004627406720374
1101	170-174	-2.898545538236874E-4
1101	175-179	0.6401349322488379
1101	180-184	0.2893784353160562
1101	185-189	0.6482382294095572
1101	190-194	0.537398397004786
1101	195-199	0.7597173885650363
1101	200-204	0.49476565773847625
1101	205-209	0.23262031831987073
1101	210-214	0.6357117625172997
1101	215-219	0.2238089590356509
1101	220-224	0.16194380232634842
1101	225-229	0.3961795479968089
1101	230-234	0.13489913674316867
1101	235-239	0.05627233660288411
1101	240-244	-0.1723538385498813
1101	245-249	-0.08482886809697732
1101	250-254	-0.037322170847431835
1101	255-259	-0.39198071503900245
1101	260-264	-0.12923952748458056
1101	265-269	-0.0444561178948959
1101	270-274	-0.34151425730312823
1101	275-279	0.15897704550754277
1101	280-284	-0.14910061868564206
1101	285-289	-0.17884609767375537
1101	290-294	-0.0826458003975663
1101	295-299	-0.016153748260311573
1101	300-301	-0.2444136884362429
1102	1	0.05909856527113533
1102	2	0.08847681136914076
1102	3	0.07725644583037905
1102	4	0.06196744816035249
1102	5	0.02531867135818544
1102	6	0.1314703813099669
1102	7	0.9398945920920809
1102	8	0.12395918428135388
1102	9	0.09983535668256849
1102	10-14	0.045871593373036035
1102	15-19	0.1628720499489802
1102	20-24	0.11763219992477758
1102	25-29	0.04514490230130974
1102	30-34	0.42245854152558593
1102	35-39	0.12091784164851305
1102	40-44	0.4924824274020949
1102	45-49	0.16449690064409594
1102	50-54	0.23672767356410418
1102	55-59	0.37918755495837075
1102	60-64	0.19068164362051476
1102	65-69	0.2134099667179541
1102	70-74	0.2525125815470446
1102	75-79	0.319396600472146
1102	80-84	0.7915546371648219
1102	85-89	0.293695735321009
1102	90-94	0.4594796761824256
1102	95-99	0.9312393891227799
1102	100-104	0.5423091527338144
1102	105-109	0.726068295509279
1102	110-114	0.8951086571508924
1102	115-119	0.6779241876619508
1102	120-124	1.0965921060493145
1102	125-129	0.8474765833439761
1102	130-134	0.8693361716831518
1102	135-139	0.9403180713902728
1102	140-144	1.610714351946875
1102	145-149	1.9985293796858876
1102	150-154	1.6814699819201557
1102	155-159	1.3756112518868768
1102	160-164	1.468704114520687
1102	165-169	1.2618698138939308
1102	170-174	1.3659422787110884
1102	175-179	1.3832950824149393
1102	180-184	1.4467767566006913
1102	185-189	1.1532662730189465
1102	190-194	1.5850956400584018
1102	195-199	1.2842592229081795
1102	200-204	1.368130380708429
1102	205-209	0.9530869015609298
1102	210-214	1.2189666217590833
1102	215-219	1.4597440911212054
1102	220-224	1.2303053893335587
1102	225-229	1.003266523698354
1102	230-234	0.6445263923106239
1102	235-239	0.8592360464618096
1102	240-244	0.42982483030145957
1102	245-249	0.4860720984997702
1102	250-254	0.789667214893992
1102	255-259	0.3607031589103009
1102	260-264	0.46119893712405613
1102	265-269	0.3878806094804066
1102	270-274	0.28861289173162596
1102	275-279	0.25756091075417054
1102	280-284	0.3042281532473403
1102	285-289	0.20340015192486938
1102	290-294	0.03920480773913049
1102	295-299	-0.040981870493835615
1102	300-301	-0.10968432652213522
1103	1	0.1479242640230467
1103	2	0.12384938356621689
1103	3	0.0673235995904804
1103	4	0.07289237284984296
1103	5	0.0160959603002766
1103	6	-0.043966178439390546
1103	7	0.924928983139857
1103	8	0.3099075539858731
1103	9	0.16116781754974596
1103	10-14	0.15054793154816082
1103	15-19	0.23924007788398427
1103	20-24	0.2492433172342814
1103	25-29	0.14152848988615574
1103	30-34	0.5925523517400748
1103	35-39	0.1782901303946005
1103	40-44	0.43464847540180074
1103	45-49	0.1431672139780531
1103	50-54	0.17118566401723
1103	55-59	0.2666976189760746
1103	60-64	0.1642552669986017
1103	65-69	0.31935700963062175
1103	70-74	0.22981026840607655
1103	75-79	0.4435255163927465
1103	80-84	0.7819755015697112
1103	85-89	0.5124948680816175
1103	90-94	0.39853650810503893
1103	95-99	0.9888082842971855
1103	100-104	0.658888321498722
1103	105-109	0.788560794612188
1103	110-114	0.6377619996109445
1103	115-119	0.57761691473182
1103	120-124	1.094388123864519
1103	125-129	0.9710105537384308
1103	130-134	1.157902241393998
1103	135-139	1.1305311722535905
1103	140-144	1.6608188368048644
1103	145-149	1.6471599194187867
1103	150-154	1.767273610695863
1103	155-159	1.1228827346069217
1103	160-164	0.8187642724692239
1103	165-169	1.9625042616319028
1103	170-174	1.953096822822392
1103	175-179	1.603094435302367
1103	180-184	1.0815284505218763
1103	185-189	1.3060095659353834
1103	190-194	1.6550489309544965
1103	195-199	1.5986503912991736
1103	200-204	1.8749914022778427
1103	205-209	1.6532789848352074
1103	210-214	1.9788999203143902
1103	215-219	2.2962052357204072
1103	220-224	1.9248718751745777
1103	225-229	0.9995792485368007
1103	230-234	2.001216287886077
1103	235-239	1.3088140361912366
1103	240-244	1.0574751724026754
1103	245-249	1.2369678337686079
1103	250-254	1.0270643508328625
1103	255-259	1.219202617639537
1103	260-264	0.7830786393195943
1103	265-269	0.761003425173941
1103	270-274	0.7261023000126166
1103	275-279	0.6826837143859645
1103	280-284	0.5413083668608287
1103	285-289	0.35870324847611457
1103	290-294	0.19198609514938347
1103	295-299	0.31180152772162906
1103	300-301	0.05028304646016046
2116	1	0.10427902728974203
2116	2	-0.03744640454317505
2116	3	0.04400723353178648
2116	4	0.06504060359512209
2116	5	0.1111295931554892
2116	6	0.26551401474135616
2116	7	0.835490930587163
2116	8	0.16950251159182272
2116	9	0.1864377130670576
2116	10-14	0.15503192607422278
2116	15-19	0.20831204278126592
2116	20-24	0.13202257130173223
2116	25-29	0.12218463829067616
2116	30-34	0.408107891205276
2116	35-39	0.05055363241112332
2116	40-44	0.43720355870556915
2116	45-49	0.06250368010262974
2116	50-54	0.0429692552386598
2116	55-59	0.31224801757428366
2116	60-64	0.2935989013743452
2116	65-69	0.3792638947720661
2116	70-74	0.22281347601745694
2116	75-79	0.45312604930515477
2116	80-84	0.6695031789839305
2116	85-89	0.40500817279021106
2116	90-94	0.459719197140501
2116	95-99	1.2495585612523499
2116	100-104	0.5871073369140873
2116	105-109	0.5551405251149077
2116	110-114	0.6887159562683678
2116	115-119	0.7538194793727868
2116	120-124	1.195133477172405
2116	125-129	0.8723276293815729
2116	130-134	0.7648981649036912
2116	135-139	1.1196093641165419
2116	140-144	1.7829012499712746
2116	145-149	1.589167753810461
2116	150-154	2.2133630038563332
2116	155-159	1.9476153733198167
2116	160-164	1.6422749777816925
2116	165-169	1.6995519558444485
2116	170-174	1.9412062146795535
2116	175-179	1.9810121669491103
2116	180-184	2.06721309091461
2116	185-189	1.772816797904337
2116	190-194	1.6096175687695577
2116	195-199	0.9075047020247595
2116	200-204	0.6355480640449969
2116	205-209	1.176710477351989
2116	210-214	1.756649958327845
2116	215-219	1.803603305361051
2116	220-224	1.1656066571221722
2116	225-229	1.0479022397973239
2116	230-234	0.9168820301621032
2116	235-239	0.9901022940165767
2116	240-244	0.8670849733569952
2116	245-249	1.007954518199023
2116	250-254	0.9199860286380748
2116	255-259	1.6184999842123151
2116	260-264	0.5647878689860182
2116	265-269	0.811933053221356
2116	270-274	0.351037966768585
2116	275-279	0.35717415309741796
2116	280-284	0.15488952377312515
2116	285-289	0.17570083733708586
2116	290-294	0.1701439270068299
2116	295-299	0.15100911013841412
2116	300-301	-0.024909736052203257
1117	1	0.143602801049731
1117	2	-0.007403342966568971
1117	3	0.08373842490207295
1117	4	-0.008742580641730058
1117	5	0.034807660749116565
1117	6	0.17267789521285692
1117	7	1.036260819380999
1117	8	0.2071893654869399
1117	9	0.30052914279838916
1117	10-14	0.08841958175559483
1117	15-19	0.15168331476514396
1117	20-24	0.27710035761771223
1117	25-29	0.22608540817946476
1117	30-34	0.5205806696193847
1117	35-39	0.2393161557905188
1117	40-44	0.5571464332470413
1117	45-49	0.1289350788064354
1117	50-54	0.2898086515066538
1117	55-59	0.3649066712113225
1117	60-64	0.43762059164996003
1117	65-69	0.30204129396882706
1117	70-74	0.33111812579780064
1117	75-79	0.49546007375020906
1117	80-84	0.9756066137608599
1117	85-89	0.4605201995840815
1117	90-94	0.530908247610995
1117	95-99	1.0982625092884746
1117	100-104	0.48490786177235634
1117	105-109	0.5546179866567158
1117	110-114	0.7884056809352558
1117	115-119	0.9541712214092968
1117	120-124	1.010531833003121
1117	125-129	1.0764887985686258
1117	130-134	1.063828149969872
1117	135-139	1.1967417820893047
1117	140-144	1.644151340589076
1117	145-149	2.073707052936175
1117	150-154	2.3147789612877183
1117	155-159	1.8357683651569658
1117	160-164	2.2620403864317105
1117	165-169	1.727147901966724
1117	170-174	1.5723656875014598
1117	175-179	1.9483787413064455
1117	180-184	1.3271327673003341
1117	185-189	1.6399708191596467
1117	190-194	-0.315541991045432
1117	195-199	1.2283578142023401
1117	200-204	1.6525844996483663
1117	205-209	1.7688260041676038
1117	210-214	2.3413664333680764
1117	215-219	2.0497044203224135
1117	220-224	1.85223060051867
1117	225-229	1.6494657954946454
1117	230-234	1.4645061040482297
1117	235-239	1.1779006440477495
1117	240-244	0.896575628849881
1117	245-249	1.3271654909266921
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2108	240-244	-0.6091553774825478
2108	245-249	-1.00536507916539
2108	250-254	-0.6515055401955827
2108	255-259	-0.8075995014042654
2108	260-264	-0.2395509801973379
2108	265-269	-0.35792393531054323
2108	270-274	-0.4502100032412173
2108	275-279	-0.2462297917011611
2108	280-284	-0.6116482251018862
2108	285-289	-0.8927409171467762
2108	290-294	-0.5013804078360291
2108	295-299	-0.46887599420487014
2108	300-301	2.5204354159377242
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	51.0
11	313.0
12	592.0
13	636.0
14	745.0
15	1196.0
16	1708.0
17	2474.0
18	3265.0
19	3512.0
20	4731.0
21	6308.0
22	8480.0
23	11923.0
24	16898.0
25	24138.0
26	33856.0
27	44604.0
28	51106.0
29	46805.0
30	31284.0
31	13580.0
32	3355.0
33	353.0
34	12.0
35	2.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.42326939784374	21.20546662862382	13.544428379535086	34.82683559399735
2	47.70380439138393	22.85968922415037	15.673438506839698	13.763067877625998
3	47.02480372135967	21.79790913957984	17.530912483289466	13.646374655771023
4	47.30018690150643	20.880072067682068	17.628691144459154	14.191049886352344
5	29.009806718836657	21.10480269548519	36.2566481474951	13.628742438183048
6	27.812418851725873	20.48190453596812	36.50638446569572	15.199292146610285
7	16.461117754360767	49.93892840999074	19.062671313023156	14.537282522625341
8	27.234082114840234	19.422689137592208	37.79545986426398	15.547768883303572
9	15.143830807651742	18.315065287292942	38.952453923809585	27.588649981245734
10-14	27.069878081230026	34.69186898300575	22.943746814178866	15.29450612158536
15-19	21.641463281708337	36.32974170404162	20.586864318482732	21.441930695767304
20-24	27.266781863821578	30.835991523712124	25.400972657239308	16.49625395522699
25-29	18.416306274825363	27.13476464195378	37.5060350272017	16.94289405601916
30-34	18.687137136976684	28.84912912874404	30.726607657511806	21.737126076767467
35-39	20.95579442757807	30.13384456078146	28.313109714069544	20.59725129757092
40-44	19.285350191870588	31.040845833506985	28.308942099003296	21.36486187561913
45-49	21.40762801791433	29.04410939668963	26.565211955284695	22.98305063011134
50-54	23.067557040223896	31.31424138185292	24.380227551782617	21.237974026140563
55-59	21.862923934613328	27.402261412052102	28.49161187321474	22.243202780119837
60-64	20.47363342299049	27.36154701871259	29.24229552237849	22.92252403591843
65-69	18.27992908642672	28.343565362630596	28.97274700332447	24.403758547618207
70-74	20.080402912201173	26.194037745769066	31.07360969964319	22.65194964238657
75-79	21.452317674855497	25.239140958359112	28.192890048696977	25.11565131808841
80-84	22.446005340959	28.00489855063171	25.17124089135669	24.3778552170526
85-89	21.193348486354267	26.51904760378163	26.65516832356081	25.63243558630329
90-94	22.310782261347935	29.814605246706783	27.510042349380786	20.364570142564496
95-99	24.120809543197332	27.7220136633657	27.600447537740962	20.55672925569601
100-104	22.931436320444718	26.480513193707544	27.8010701153147	22.786980370533037
105-109	24.55481856448102	28.0140031866226	24.35272129234537	23.078456956551012
110-114	21.607160603855363	26.84072336974119	29.795883037486092	21.75623298891735
115-119	25.394689176062503	26.724799553744603	27.33263018186831	20.547881088324587
120-124	20.38354882040464	27.4666991526918	27.053207620965026	25.09654440593853
125-129	19.565093338548195	24.44729409577179	30.50687816778818	25.48073439789183
130-134	24.019376204200316	28.91061748025993	26.705756758749587	20.364249556790167
135-139	23.33633615341953	26.106197243603514	25.157712171680092	25.399754431296866
140-144	21.70250281314017	27.50536179707562	26.74666350355369	24.045471886230523
145-149	22.83058003584149	25.027169644374204	29.32898191575647	22.813268404027838
150-154	21.945635064389652	27.45945391419201	25.201311836988545	25.39359918442979
155-159	21.707760419839133	26.32900435676067	30.78238958224468	21.180845641155518
160-164	23.216885893905346	30.431925213750567	24.491406698319167	21.85978219402492
165-169	21.616714059930306	28.307082701512204	26.527895771153048	23.54830746740444
170-174	22.002378746445505	30.2372655315793	26.079332155714923	21.681023566260272
175-179	19.720898024871044	28.169423170016255	28.09774019087677	24.01193861423593
180-184	21.639603884217244	28.432303504964267	27.123992959936395	22.804099650882094
185-189	21.56978030256885	26.882655989023142	28.121655889641552	23.42590781876645
190-194	20.803259716153356	25.83318639818677	27.77260209855448	25.590951787105396
195-199	20.508320803772655	31.07187853646182	26.252704942470885	22.16709571729464
200-204	22.374899416213303	26.679404608099922	25.295948757569832	25.649747218116943
205-209	22.27680016926929	26.10164492560807	28.624783203870113	22.996771701252527
210-214	24.622333928554255	25.383083971031866	27.98309871797749	22.011483382436385
215-219	23.304918747535496	26.998002750625943	28.36433932080698	21.33273918103158
220-224	21.928387549730868	26.65863064992354	26.729544223204638	24.68343757714095
225-229	24.715752623193097	26.277902984332975	26.84085160405092	22.165492788423006
230-234	23.788618563839847	26.22391633993633	27.219206934911472	22.76825816131235
235-239	23.543306329324942	26.448262264810264	25.366028807837683	24.642402598027115
240-244	24.13728765199773	29.183692442831543	24.641825543633328	22.037194361537402
245-249	24.820135351313922	27.39674733673368	24.018927384116257	23.764189927836142
250-254	22.645666161209764	27.190738918151247	23.62024691516339	26.543348005475604
255-259	23.114939617669407	28.31221207390143	23.74495478137653	24.82789352705263
260-264	21.567407967838836	27.518762282442477	25.46021690833491	25.453612841383777
265-269	21.979104219229377	28.018491387463172	24.404207367702266	25.598197025605185
270-274	20.998432335563542	29.882697665173808	23.466686329260824	25.652183670001826
275-279	21.610174110134036	28.629143170400955	23.79438910777773	25.966293611687274
280-284	19.608180066617724	29.273648811107655	26.238983871329697	24.879187250944927
285-289	20.97105431043603	29.32904603291134	23.951604371507617	25.74829528514502
290-294	21.487389758566852	29.562945413860202	23.648137877529823	25.30152695004312
295-299	20.35629902958686	29.824158702781723	22.97279188533288	26.846750382298534
300-301	16.267804532441676	30.857823414943788	28.637125756181696	24.237246296432843
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.25
26	0.25
27	0.0
28	0.5
29	2.75
30	2.75
31	5.25
32	20.25
33	68.0
34	142.75
35	235.75
36	423.25
37	831.0
38	1657.0
39	3137.75
40	4750.0
41	5690.25
42	6545.25
43	8258.25
44	9830.75
45	10160.5
46	9958.75
47	9726.0
48	8939.25
49	7608.5
50	6304.5
51	4555.5
52	2631.5
53	1208.5
54	520.25
55	219.5
56	119.0
57	74.75
58	51.25
59	39.0
60	40.75
61	34.75
62	29.5
63	24.75
64	17.5
65	14.25
66	12.5
67	6.25
68	9.5
69	7.25
70	3.75
71	3.0
72	3.0
73	2.25
74	1.5
75	2.0
76	0.75
77	1.5
78	1.5
79	1.5
80	0.5
81	1.0
82	0.25
83	1.25
84	1.75
85	1.0
86	1.25
87	0.5
88	0.5
89	0.25
90	0.5
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.5
97	0.0
98	0.5
99	0.5
100	1.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	311929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.627171876193218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.57023767308971	6.958180426356448
2	7.599566543029577	2.223203320189223
3	6.744089652976455	2.959408755076286
4	5.891822619478254	3.4472280847660546
5	4.868406518169362	3.560550945222132
6	4.100465705837556	3.598692999103312
7	3.387485342887221	3.468453123694769
8	2.7127013605899473	3.174331924010588
9	2.124777231890797	2.797153359253987
>10	14.63146001194886	37.97342464686393
>50	0.28017722749869245	2.5889574092402037
>100	0.041041863254826755	1.0772392699192828
>500	0.006824035621283613	0.7146850081587325
>1k	0.027296142484979195	8.8131175493636
>5k	0.009098714161659732	8.8104557802457
>10k+	0.004549357080829866	7.834917398535716
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	11832	3.793170881835289	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	11716	3.755982932013375	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	7506	2.4063168220973363	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	7449	2.388043432960706	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	5901	1.8917766543027421	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	5624	1.8029743948142045	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	4879	1.5641379929407013	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	3359	1.0768476159638891	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	2871	0.9204017580923864	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	2667	0.8550022601297089	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	2141	0.6863741428337858	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	2075	0.6652154817282139	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	1892	0.6065482850264002	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	1856	0.5950071971506337	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	1492	0.4783139752956602	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	1199	0.3843823434178932	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	1040	0.333409205299924	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	1017	0.32603573249040646	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT	861	0.27602435169541784	Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	785	0.2516598328465772	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	502	0.1609340587120787	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	471	0.15099589970794636	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	356	0.1141285356603586	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	3.2058577432685E-4	0.0	0.0
6	3.2058577432685E-4	0.0	0.0
7	3.2058577432685E-4	0.0	0.0
8	6.411715486537E-4	0.0	0.0
9	9.6175732298055E-4	0.0	0.0
10-14	0.00153881171676888	0.0	0.0
15-19	0.00166704602649962	0.0	0.0
20-24	0.00224410042028795	0.0	0.0
25-29	0.00224410042028795	0.0	0.0
30-34	0.005449958163556451	0.0	0.0
35-39	0.00743758996438292	0.0	0.0
40-44	0.01166932218549734	0.0	0.0
45-49	0.015067531393361953	0.0	0.0
50-54	0.02186394980909117	0.0	0.0
55-59	0.029558008392935577	0.0	0.0
60-64	0.03981675317139478	0.0	0.0
65-69	0.05616662766206413	0.0	0.0
70-74	0.07847939755521288	0.0	0.0
75-79	0.11246148963385898	0.0	0.0
80-84	0.16163934741559777	0.0	0.0
85-89	0.2321682177675048	0.0	0.0
90-94	0.3289851216142135	0.0	0.0
95-99	0.4424724857259184	0.0	0.0
100-104	0.5707709126115238	0.0	0.0
105-109	0.7365137579385053	0.0	0.0
110-114	0.9411757162687664	0.0	0.0
115-119	1.177319197637924	0.0	0.0
120-124	1.4605246706782635	0.0	0.0
125-129	1.7882274491951695	0.0	0.0
130-134	2.1670316001397754	0.0	0.0
135-139	2.5590438849866475	0.0	0.0
140-144	2.9992081531374124	0.0	0.0
145-149	3.527469392073196	0.0	0.0
150-154	4.125874798431694	0.0	0.0
155-159	4.76890574457649	0.0	0.0
160-164	5.502213644771727	0.0	0.0
165-169	6.281750013624896	0.0	0.0
170-174	7.11200305197657	0.0	0.0
175-179	7.947000759788286	0.0	0.0
180-184	8.84361505342562	0.0	0.0
185-189	9.763503874279085	0.0	0.0
190-194	10.744303992254647	0.0	0.0
195-199	11.721449432402887	0.0	0.0
200-204	12.618704897588874	0.0	0.0
205-209	13.478259475714022	0.0	0.0
210-214	14.373014371860261	0.0	0.0
215-219	15.18460931814612	0.0	0.0
220-224	15.96754389620715	0.0	0.0
225-229	16.6921318633407	0.0	0.0
230-234	17.34099747057824	0.0	0.0
235-239	17.891699713716903	0.0	0.0
240-244	18.361229638796008	0.0	0.0
245-249	18.740546726979534	0.0	0.0
250-254	19.060234861138273	0.0	0.0
255-259	19.297083631210945	0.0	0.0
260-264	19.494756819660886	0.0	0.0
265-269	19.65479323820485	0.0	0.0
270-274	19.775012903577416	0.0	0.0
275-279	19.858429322057265	0.0	0.0
280-284	19.91331360662202	0.0	3.2058577432685E-4
285-289	19.95678503762074	2.5646861946148E-4	3.2058577432685E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	5540	0.0	292.07132	1
GGGTTAT	5595	0.0	290.2547	2
GGTTATT	5755	0.0	281.92874	3
ATTAGTA	5730	0.0	281.09946	7
TTATTAG	5770	0.0	279.66205	5
TATTAGT	5835	0.0	276.2939	6
GTTATTA	5900	0.0	274.5	4
GGGGGGA	3655	0.0	272.80438	1
TTAGTAG	6125	0.0	266.34286	8
GGAGCGA	2875	0.0	265.7565	5
GAGCGAA	2865	0.0	265.65445	6
CGAAAGC	2905	0.0	265.04303	9
GCGAAAG	2910	0.0	263.57388	8
TAGTAGA	6260	0.0	260.12778	9
GGGGGAG	3890	0.0	255.94473	2
AGCGAAA	3225	0.0	234.6279	7
GGGGAGC	4410	0.0	224.7619	3
GGGAGCG	3510	0.0	221.46011	4
GGCCCGC	45	1.7833372E-8	163.88889	295
GAGCAAA	1295	0.0	148.0695	6
>>END_MODULE