FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_7765_flu.3520000003b9ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_7765_flu.3520000003b9ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311929
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA118323.793170881835289No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC117163.755982932013375No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC75062.4063168220973363No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG74492.388043432960706No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG59011.8917766543027421No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA56241.8029743948142045No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG48791.5641379929407013No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG33591.0768476159638891No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC28710.9204017580923864No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA26670.8550022601297089No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG21410.6863741428337858No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG20750.6652154817282139No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA18920.6065482850264002No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA18560.5950071971506337No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA14920.4783139752956602No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT11990.3843823434178932No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA10400.333409205299924No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC10170.32603573249040646No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT8610.27602435169541784Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA7850.2516598328465772No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT5020.1609340587120787No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA4710.15099589970794636No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA3560.1141285356603586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA55400.0292.071321
GGGTTAT55950.0290.25472
GGTTATT57550.0281.928743
ATTAGTA57300.0281.099467
TTATTAG57700.0279.662055
TATTAGT58350.0276.29396
GTTATTA59000.0274.54
GGGGGGA36550.0272.804381
TTAGTAG61250.0266.342868
GGAGCGA28750.0265.75655
GAGCGAA28650.0265.654456
CGAAAGC29050.0265.043039
GCGAAAG29100.0263.573888
TAGTAGA62600.0260.127789
GGGGGAG38900.0255.944732
AGCGAAA32250.0234.62797
GGGGAGC44100.0224.76193
GGGAGCG35100.0221.460114
GGCCCGC451.7833372E-8163.88889295
GAGCAAA12950.0148.06956
Produced by FastQC (version 0.11.2)