##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_7637_flu.3520000003b9c7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329597
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.38503991237784	34.0	34.0	34.0	34.0	34.0
2	33.55796017560839	34.0	34.0	34.0	34.0	34.0
3	33.57243239471233	34.0	34.0	34.0	34.0	34.0
4	33.58279049869993	34.0	34.0	34.0	34.0	34.0
5	33.53145204598343	34.0	34.0	34.0	34.0	34.0
6	37.11012236155062	38.0	38.0	38.0	37.0	38.0
7	36.20297211443065	38.0	38.0	38.0	34.0	38.0
8	37.00349821145217	38.0	38.0	38.0	36.0	38.0
9	37.077518909456096	38.0	38.0	38.0	37.0	38.0
10-14	37.10823945606301	38.0	38.0	38.0	37.0	38.0
15-19	37.025378871773704	38.0	38.0	38.0	36.8	38.0
20-24	37.12916136979402	38.0	38.0	38.0	36.8	38.0
25-29	37.04814910329888	38.0	38.0	38.0	37.0	38.0
30-34	36.711847498611945	38.0	38.0	38.0	34.8	38.0
35-39	37.00023786624271	38.0	38.0	38.0	36.4	38.0
40-44	36.53618570557378	38.0	38.0	38.0	34.8	38.0
45-49	36.83590323941055	38.0	38.0	38.0	35.8	38.0
50-54	36.81611240393572	38.0	38.0	38.0	35.6	38.0
55-59	36.477155435273986	38.0	37.4	38.0	34.4	38.0
60-64	36.64391120064806	38.0	37.8	38.0	34.8	38.0
65-69	36.53012497079767	38.0	37.6	38.0	34.4	38.0
70-74	36.43730616480126	38.0	37.0	38.0	34.0	38.0
75-79	36.49377633898367	38.0	37.0	38.0	34.0	38.0
80-84	35.83989296019079	38.0	37.0	38.0	31.2	38.0
85-89	36.03142929092195	38.0	37.0	38.0	32.2	38.0
90-94	36.04304650831166	38.0	37.0	38.0	32.2	38.0
95-99	35.14829321868828	38.0	36.4	38.0	27.8	38.0
100-104	35.51165392888892	38.0	36.6	38.0	29.8	38.0
105-109	35.356515987706196	38.0	36.2	38.0	28.6	38.0
110-114	34.89338980633926	38.0	35.6	38.0	26.0	38.0
115-119	34.94485265339186	38.0	35.4	38.0	26.8	38.0
120-124	34.48675200320391	38.0	34.6	38.0	24.8	38.0
125-129	34.06851822073624	37.0	34.0	38.0	23.0	38.0
130-134	33.92874935148075	37.0	34.0	38.0	23.0	38.0
135-139	33.29567866212375	37.0	32.8	38.0	22.0	38.0
140-144	32.008377503436016	36.4	28.8	38.0	17.4	38.0
145-149	31.87099397142571	35.8	28.6	37.6	18.8	38.0
150-154	30.84887787206801	35.0	27.0	37.2	14.2	38.0
155-159	30.660431375285576	35.0	26.2	37.0	15.0	37.6
160-164	29.90510107798311	35.0	24.4	37.0	11.0	37.4
165-169	28.811064421096063	33.4	23.2	36.6	10.0	37.0
170-174	28.125872504907505	32.6	21.8	36.2	9.4	37.0
175-179	27.2878387849404	30.8	20.2	36.2	9.2	37.0
180-184	26.33597999981796	28.6	20.0	35.0	9.0	37.0
185-189	25.557503253973795	27.8	18.6	35.0	9.0	36.8
190-194	24.464737846521665	25.8	16.0	34.6	8.4	36.0
195-199	23.514240117476795	24.6	15.0	33.6	8.0	36.0
200-204	22.608414518336026	23.4	14.0	32.0	8.0	36.0
205-209	21.677685779906977	22.4	10.2	31.0	8.0	35.4
210-214	20.33801035810399	20.4	9.0	28.6	8.0	34.8
215-219	19.730474488542065	19.6	9.0	27.8	8.0	34.0
220-224	18.956101542186367	18.2	9.0	26.2	8.0	33.4
225-229	18.068975749172473	16.8	9.0	25.4	8.0	32.2
230-234	17.138824685904304	15.6	8.6	24.2	7.2	31.0
235-239	16.178393614019548	14.2	8.0	22.6	7.0	29.2
240-244	15.262886494719307	13.0	8.0	20.8	7.0	28.2
245-249	14.559002054023551	12.2	8.0	19.8	7.0	26.4
250-254	13.84055194677136	11.4	8.0	18.6	7.0	25.2
255-259	13.251889428605235	10.6	8.0	17.0	7.0	24.0
260-264	12.667342239158732	9.0	7.4	15.8	7.0	22.8
265-269	12.273767054918583	9.0	7.0	15.2	7.0	21.8
270-274	11.809328968406872	8.4	7.0	14.0	7.0	21.0
275-279	11.36028119187978	8.0	7.0	13.2	7.0	19.8
280-284	11.20235985157632	8.0	7.0	13.0	7.0	19.0
285-289	10.947971007017662	8.0	7.0	13.0	7.0	19.0
290-294	10.723572726693508	8.0	7.0	13.0	7.0	18.4
295-299	10.48403717266844	8.0	7.0	13.0	7.0	17.8
300-301	10.215721016878188	8.0	7.0	12.5	7.0	15.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.15945392457206964
1101	2	0.10067628129438333
1101	3	0.21681612996199107
1101	4	0.16156393853991347
1101	5	0.196845723122955
1101	6	0.13219535320616416
1101	7	1.139628889715837
1101	8	0.4393334037059873
1101	9	0.23101608979617083
1101	10-14	0.2872254306271671
1101	15-19	0.33135281378924475
1101	20-24	0.2805167894925802
1101	25-29	0.17792764694421237
1101	30-34	0.546089957718408
1101	35-39	0.2832508360281025
1101	40-44	0.47642888602286604
1101	45-49	0.14531848271582248
1101	50-54	0.1633243528570958
1101	55-59	0.3377811266055275
1101	60-64	0.2060980413041662
1101	65-69	0.21768752067069386
1101	70-74	0.07445116336660362
1101	75-79	0.371602669066597
1101	80-84	0.6551906196717141
1101	85-89	0.21781156129143397
1101	90-94	0.28083928569955674
1101	95-99	0.7966727836365877
1101	100-104	0.3953167479817594
1101	105-109	0.5384046928164992
1101	110-114	0.3468299552134084
1101	115-119	0.2795513946486139
1101	120-124	0.8607700457014218
1101	125-129	0.6579179129691184
1101	130-134	0.6290152269376605
1101	135-139	0.47606389093545687
1101	140-144	1.1772618749854793
1101	145-149	1.2051975708804186
1101	150-154	0.8246478657852379
1101	155-159	0.43155520430304506
1101	160-164	0.6629723871465032
1101	165-169	0.5521094021564892
1101	170-174	0.13064041759091793
1101	175-179	0.558966897955667
1101	180-184	0.5950344212148266
1101	185-189	0.6227908353685265
1101	190-194	0.6876553110988972
1101	195-199	0.36450677905779116
1101	200-204	0.6789499213165406
1101	205-209	0.5623606349418964
1101	210-214	0.832882281487386
1101	215-219	0.8510263836273815
1101	220-224	0.2302240567861915
1101	225-229	0.4098486319909931
1101	230-234	0.364695642002129
1101	235-239	0.3074637425054405
1101	240-244	0.1520210353368885
1101	245-249	0.009318120756912407
1101	250-254	0.1108724934673706
1101	255-259	0.024506179436995623
1101	260-264	-0.010176176045840535
1101	265-269	0.04219606511613172
1101	270-274	0.08165232879025908
1101	275-279	0.2407568016813979
1101	280-284	0.10543347514434309
1101	285-289	0.2616340285265153
1101	290-294	0.18813153139505623
1101	295-299	0.08542691266706193
1101	300-301	0.021119605164512834
1102	1	-0.05511005423963411
1102	2	-0.018202188992759716
1102	3	0.011923775820910976
1102	4	-0.018836886050635826
1102	5	-0.03511094799852543
1102	6	-0.16425414603362753
1102	7	0.9379099106065496
1102	8	0.08312543807475237
1102	9	0.064858930629633
1102	10-14	0.004861063327275872
1102	15-19	0.033425652620742596
1102	20-24	0.020480754236466225
1102	25-29	-0.11942760332766511
1102	30-34	0.35614141346701444
1102	35-39	0.14196720176972377
1102	40-44	0.3527581595282925
1102	45-49	0.10518353987985307
1102	50-54	0.04114427396909548
1102	55-59	0.10941012578851428
1102	60-64	0.1186768346100493
1102	65-69	0.10535816059407921
1102	70-74	0.004411286514113044
1102	75-79	0.2337532055374183
1102	80-84	0.6933977304124994
1102	85-89	0.25993493605268725
1102	90-94	0.30060511064178996
1102	95-99	0.7781557480504162
1102	100-104	0.6323264710080707
1102	105-109	0.8215108669652622
1102	110-114	0.6259554780222061
1102	115-119	0.24826997877686807
1102	120-124	0.8405107649997632
1102	125-129	0.8093136028062062
1102	130-134	1.0583368875894337
1102	135-139	0.8223210398216665
1102	140-144	1.5642653757078087
1102	145-149	1.5389148834943818
1102	150-154	1.4946520320235628
1102	155-159	1.0819017595814486
1102	160-164	1.4784242616832088
1102	165-169	1.47094783327017
1102	170-174	1.3633237456037328
1102	175-179	1.1662686364132888
1102	180-184	1.3132343898327754
1102	185-189	1.3201276892634155
1102	190-194	1.4899597278525896
1102	195-199	1.281717671877253
1102	200-204	1.2999012304351858
1102	205-209	1.4059871019176953
1102	210-214	1.5558945258994896
1102	215-219	1.6755963683151016
1102	220-224	1.5322476582557378
1102	225-229	1.5093481423227182
1102	230-234	1.158194687013733
1102	235-239	0.991809507821781
1102	240-244	0.9449032606492214
1102	245-249	0.7755228940756727
1102	250-254	1.1035220587176955
1102	255-259	0.9395603675886495
1102	260-264	0.7124259373732347
1102	265-269	0.4192552419323672
1102	270-274	0.3985867348848693
1102	275-279	0.15626278320927156
1102	280-284	0.4366207448514636
1102	285-289	0.32721007860794593
1102	290-294	0.06906368663883633
1102	295-299	-0.08330357499924901
1102	300-301	-0.21609897084769436
1103	1	0.03160458943865052
1103	2	0.06595778752604531
1103	3	0.04436261328429225
1103	4	0.09750374348458024
1103	5	0.1533283677855124
1103	6	0.16656981565723328
1103	7	1.0838384777644023
1103	8	0.46426003642127966
1103	9	0.36298858814117096
1103	10-14	0.2659502212111349
1103	15-19	0.3302779765525372
1103	20-24	0.21566167359051747
1103	25-29	0.2275369628952717
1103	30-34	0.6198330705763269
1103	35-39	0.35025302010570414
1103	40-44	0.6303151367591511
1103	45-49	0.28966203447428995
1103	50-54	0.2808840240465571
1103	55-59	0.41722577665790794
1103	60-64	0.3613473858202596
1103	65-69	0.46729579410261834
1103	70-74	0.5095168160096435
1103	75-79	0.4179452512822621
1103	80-84	0.9633994154057248
1103	85-89	0.49671670768719167
1103	90-94	0.6081267030147472
1103	95-99	1.2512632560541377
1103	100-104	0.6854466979929725
1103	105-109	0.9118569428334453
1103	110-114	0.7866244746902709
1103	115-119	0.8661597149938842
1103	120-124	1.2797303413598513
1103	125-129	1.3722676677164927
1103	130-134	1.2426039427104527
1103	135-139	1.3816001888457237
1103	140-144	1.8007117793343461
1103	145-149	1.9717358339392739
1103	150-154	2.42855627005331
1103	155-159	1.2036365416466026
1103	160-164	1.3880944922011622
1103	165-169	2.1771872001475536
1103	170-174	2.315679746625598
1103	175-179	1.8696391664251948
1103	180-184	1.4887954654020028
1103	185-189	1.5915116122537931
1103	190-194	1.5330075001284733
1103	195-199	1.6571194884086644
1103	200-204	1.5457743558802122
1103	205-209	1.4781162267539543
1103	210-214	1.7859939405002265
1103	215-219	1.787244019923726
1103	220-224	1.5942342109226502
1103	225-229	0.8338479439283546
1103	230-234	1.3555329412520365
1103	235-239	1.131341079703045
1103	240-244	0.7665146577260291
1103	245-249	0.9635953161719133
1103	250-254	0.49424535565155203
1103	255-259	0.5977931901697335
1103	260-264	0.4028203888535842
1103	265-269	0.5090767957135487
1103	270-274	0.2520640717258864
1103	275-279	0.4426708668284185
1103	280-284	0.23788448977609988
1103	285-289	0.25393189709612685
1103	290-294	-0.04556477235332146
1103	295-299	-0.09597037574064693
1103	300-301	-0.20895133826318535
2116	1	-0.0029720177106895562
2116	2	0.012630888497930925
2116	3	0.03623151950429104
2116	4	0.0164263086967793
2116	5	0.1855465945117487
2116	6	0.2013072804371987
2116	7	0.9692896834182818
2116	8	0.3012541780901543
2116	9	0.15352860277402414
2116	10-14	0.10377252022991001
2116	15-19	0.19500293594896334
2116	20-24	0.2055546254318159
2116	25-29	0.15235106855279668
2116	30-34	0.5358275080156787
2116	35-39	0.11799016835936271
2116	40-44	0.3842558892615173
2116	45-49	0.3025109268916708
2116	50-54	0.2438831367351142
2116	55-59	0.38422713097538264
2116	60-64	0.3069053801850501
2116	65-69	0.44109156717919973
2116	70-74	0.3226967638087501
2116	75-79	0.5197847361227232
2116	80-84	0.7352648969148845
2116	85-89	0.4786243754072075
2116	90-94	0.5125295320334686
2116	95-99	1.2353212270686242
2116	100-104	0.4620133734808647
2116	105-109	0.6209474745457513
2116	110-114	0.7452648644846391
2116	115-119	0.868105054533217
2116	120-124	1.0517322924072445
2116	125-129	0.9430180426185473
2116	130-134	0.9745066809224454
2116	135-139	1.5383782623939837
2116	140-144	1.9973353257780992
2116	145-149	2.0662469564660846
2116	150-154	2.36340529738354
2116	155-159	2.3510573711530753
2116	160-164	2.0085996889404143
2116	165-169	1.7782794753490165
2116	170-174	1.6878690189783256
2116	175-179	2.161770114914429
2116	180-184	2.0914662209658594
2116	185-189	1.5982307911534193
2116	190-194	1.90643330065463
2116	195-199	1.0924619854379891
2116	200-204	1.2023007650708948
2116	205-209	1.116135706094127
2116	210-214	2.094991589903234
2116	215-219	2.314456979859795
2116	220-224	1.1520552645920148
2116	225-229	0.7985386562163193
2116	230-234	1.1597162774392977
2116	235-239	0.7711045670964758
2116	240-244	0.7859581941032037
2116	245-249	0.9346715574998683
2116	250-254	0.8890268227769216
2116	255-259	1.536917917100931
2116	260-264	0.36243267007423263
2116	265-269	0.3668516676760625
2116	270-274	0.0982737989424809
2116	275-279	0.2647104378055527
2116	280-284	0.20910446518044878
2116	285-289	0.04772434924098157
2116	290-294	0.09975349225229202
2116	295-299	0.08830812589646797
2116	300-301	-0.21915023094001462
1117	1	0.1045559229384736
1117	2	0.06695670197105841
1117	3	0.13176253072095534
1117	4	0.09023103474459049
1117	5	0.06117851023275733
1117	6	0.23440426087747568
1117	7	1.1438650815062488
1117	8	0.3603474516282219
1117	9	0.3426866713466694
1117	10-14	0.14688318691677438
1117	15-19	0.24682835802573777
1117	20-24	0.2600455829135484
1117	25-29	0.19330324749415695
1117	30-34	0.47876545528069414
1117	35-39	0.24182595360144887
1117	40-44	0.42043454780733924
1117	45-49	0.2393259201495539
1117	50-54	0.255823503082091
1117	55-59	0.41843466699647536
1117	60-64	0.38422762311340364
1117	65-69	0.3742708671100061
1117	70-74	0.2904457045134663
1117	75-79	0.5166838669703182
1117	80-84	0.8397424711665593
1117	85-89	0.4939635771606632
1117	90-94	0.49459650878704764
1117	95-99	1.158409571537824
1117	100-104	0.5255306883503863
1117	105-109	0.7502239215759374
1117	110-114	0.7398120820353569
1117	115-119	0.8752325083358201
1117	120-124	1.0112639298580604
1117	125-129	1.104790487305408
1117	130-134	1.0234665766847613
1117	135-139	1.0573627529979461
1117	140-144	1.2667094451363106
1117	145-149	1.9564534622309715
1117	150-154	2.2100824686232308
1117	155-159	1.5558364468516643
1117	160-164	2.0204516754662833
1117	165-169	1.2701606524672258
1117	170-174	1.268923415495653
1117	175-179	1.1979002908155252
1117	180-184	1.6857518342460622
1117	185-189	1.0845839367175074
1117	190-194	-1.071129840414713
1117	195-199	0.974175442907093
1117	200-204	1.3898376952104314
1117	205-209	1.6649754052997174
1117	210-214	1.9481406891866015
1117	215-219	1.4288982426306802
1117	220-224	1.3895510349862903
1117	225-229	1.2208987509594884
1117	230-234	1.179440633402912
1117	235-239	1.0961675692832493
1117	240-244	0.6407324720400638
1117	245-249	0.7422898980803492
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2108	240-244	-0.893987999063329
2108	245-249	-1.1231869305281101
2108	250-254	-0.6167758598780626
2108	255-259	-0.6319825018237015
2108	260-264	-0.35755272521382686
2108	265-269	-0.468757694712961
2108	270-274	-0.25596886987645995
2108	275-279	-0.09746643294274726
2108	280-284	-0.5948872331938162
2108	285-289	-0.8792920618432252
2108	290-294	-0.585610280375402
2108	295-299	-0.5838983386102363
2108	300-301	2.537383455981084
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	20.0
11	236.0
12	479.0
13	534.0
14	606.0
15	957.0
16	1537.0
17	2336.0
18	2245.0
19	2862.0
20	4107.0
21	5876.0
22	8345.0
23	12130.0
24	17366.0
25	24508.0
26	34883.0
27	47206.0
28	55900.0
29	52865.0
30	35546.0
31	15157.0
32	3517.0
33	363.0
34	8.0
35	5.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.47084773223057	22.952878818678567	14.387266874395094	32.18900657469577
2	44.16696753914629	24.087294483869695	17.681896376484012	14.063841600500005
3	43.297724190450765	22.972599871964857	19.063583709803183	14.6660922277812
4	43.56259310612657	22.015370285530512	19.044772858976263	15.377263749366652
5	28.929874968522164	22.1206503699973	34.08708210329584	14.862392558184693
6	27.992366435374112	21.44861755416463	34.48817798705692	16.070838023404338
7	17.18856664350707	46.9203299787316	20.375185453751097	15.51591792401023
8	27.20686171294035	20.903102880183983	35.384120607893884	16.505914798981784
9	16.001966037312233	19.402179024687722	36.78037118056293	27.815483757437114
10-14	26.285858184388815	33.92731123159495	23.5900205402355	16.196810043780737
15-19	21.808693647090234	34.358989917990755	22.297168966950547	21.535147467968457
20-24	25.122740801645644	30.97776982193406	26.49314162446867	17.406347751951625
25-29	19.528939887195577	25.979787437385653	36.739108669071626	17.752164006347147
30-34	20.07870217265327	28.119491378865707	31.05131418065092	20.750492267830108
35-39	20.76784679472204	29.632915348137274	27.689572417224674	21.90966543991602
40-44	20.237380801402928	28.675018279899394	29.486190711687303	21.601410207010378
45-49	21.498496648937945	29.968901415971626	25.91862183211619	22.61398010297424
50-54	22.238673288895228	29.41416335706939	27.587811782267437	20.759351571767947
55-59	21.890733228761185	26.88028107051945	29.794992066068566	21.4339936346508
60-64	22.320773550730134	25.57286625788463	29.535159604001247	22.571200587383988
65-69	20.161227195635885	26.839321959847933	29.046441563485104	23.953009281031076
70-74	20.879012855092736	26.25333361650743	29.41616580248	23.451487725919836
75-79	20.174880232526387	26.07754318152168	30.447425188942862	23.300151397009074
80-84	23.089409187583627	26.18603931467823	27.454618822380056	23.26993267535809
85-89	20.922459852486522	27.51008049223749	25.97802771263088	25.589431942645106
90-94	20.258376138132324	30.17048092063945	28.541582599356186	21.029560341872045
95-99	22.764102828605843	29.445231601015788	26.54726833071903	21.24339723965934
100-104	22.994444730989663	28.117185532635308	26.627062746323542	22.261306990051487
105-109	23.6635042187884	27.481257414357536	23.791600044903323	25.063638321950748
110-114	22.00080704618064	28.11445492525721	28.748744679108125	21.13599334945403
115-119	23.660348850262594	28.229140435137456	26.74193029669566	21.36858041790429
120-124	19.336523087285382	28.31803687533564	27.77968246070201	24.565757576676972
125-129	21.096611923045415	24.765638036753977	30.69445413641508	23.443295903785533
130-134	25.256297842516773	28.51852413705222	26.30205978816555	19.923118232265463
135-139	24.278801081320523	27.78653931922924	24.70993364624071	23.22472595320953
140-144	21.446918509573813	29.110762537280376	26.337497003916905	23.104821949228906
145-149	21.50341174221853	26.79545020130644	28.844497977833537	22.856640078641494
150-154	23.567811600226943	25.473229428665917	25.921049038674504	25.03790993243264
155-159	22.523505978513153	26.549816897605254	29.59201691762971	21.334660206251876
160-164	25.219829063978132	29.205726993874336	25.793620694363117	19.780823247784415
165-169	21.233991814245883	25.539795568527623	28.160632530029094	25.065580087197397
170-174	23.049845720683138	29.056393110374184	25.708486424330317	22.18527474461236
175-179	21.19400358619769	29.167316450089046	27.966516685528088	21.672163278185177
180-184	21.03848032597384	29.12860250548397	26.430216294444424	23.40270087409776
185-189	23.04784327527253	27.436293412864803	27.27773614444306	22.238127167419606
190-194	21.56239286158551	27.689451056896758	25.6624908600503	25.08566522146743
195-199	22.75421196188072	29.378119339678456	26.56832434761239	21.299344350828438
200-204	24.136688137331348	27.5921807540724	26.32293376456703	21.948197344029225
205-209	23.32436278242824	27.406378092033606	27.00461472646899	22.264644399069166
210-214	24.98675655421621	27.328464761511782	26.319535675385396	21.365243008886612
215-219	25.20186773544662	26.845147255587886	26.878339305272803	21.074645703692692
220-224	22.388917374854746	27.158074861118276	26.90570605921777	23.547301704809207
225-229	24.361022703483346	28.240244905141733	25.03590748702203	22.36282490435289
230-234	24.74482474051645	26.555884914001037	26.554914031377713	22.1443763141048
235-239	24.745795623139774	27.375491888579084	24.07443028911064	23.8042821991705
240-244	25.79981007108681	30.310166658070308	22.29486312072015	21.595160150122723
245-249	24.659508429991778	29.32004842277084	22.61094609477635	23.409497052461038
250-254	25.097679893931073	27.680409712467043	23.45112364493609	23.770786748665795
255-259	25.006598967830413	28.126408917556894	24.679472203933894	22.187519910678798
260-264	24.635357724736572	29.135884125158906	23.986565411699743	22.24219273840478
265-269	23.772728513912444	29.07544668185694	24.367940242174534	22.783884562056087
270-274	23.124496354333537	30.551449528636198	22.636263458868534	23.68779065816173
275-279	23.781223736866536	30.11125708061663	22.14935208754934	23.958167094967493
280-284	21.835150198575835	30.71478199134094	24.07364144697919	23.376426363104034
285-289	23.089894628895287	30.362048198254232	23.988628537274305	22.55942863557617
290-294	22.402084970433588	31.904720006553454	22.29079754973498	23.402397473277972
295-299	21.510693361893463	33.11880872702118	20.68526109157547	24.685236819509885
300-301	17.74652075109907	35.604844704290386	27.039081059597024	19.609553485013517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.25
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.25
19	0.25
20	0.0
21	0.25
22	0.25
23	0.25
24	0.25
25	0.0
26	0.0
27	0.0
28	0.5
29	0.5
30	1.75
31	2.0
32	3.75
33	9.25
34	21.25
35	94.5
36	311.0
37	824.5
38	1506.0
39	2289.25
40	3438.0
41	5059.5
42	7513.5
43	10741.75
44	13347.75
45	13527.75
46	11859.5
47	9809.75
48	8269.0
49	6903.25
50	5654.5
51	4144.75
52	2383.75
53	1059.0
54	422.0
55	171.0
56	94.75
57	61.5
58	46.75
59	35.25
60	32.25
61	22.25
62	17.75
63	15.0
64	11.5
65	9.0
66	4.5
67	5.5
68	5.0
69	4.25
70	4.25
71	3.25
72	2.0
73	2.5
74	1.75
75	1.0
76	0.0
77	0.5
78	0.25
79	0.75
80	0.5
81	0.75
82	0.5
83	0.25
84	0.75
85	1.25
86	0.75
87	0.75
88	0.25
89	0.5
90	0.5
91	0.75
92	0.0
93	0.25
94	0.25
95	0.25
96	0.25
97	0.25
98	0.75
99	0.25
100	1.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	6.068016395780302E-5
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	329597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.165611124916566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.37733188322753	6.4279763734407895
2	7.013317446179148	1.9869585527633353
3	6.200813923412331	2.6351495609108175
4	5.606768209582642	3.1769319249796966
5	4.818043102635441	3.412526248751006
6	4.426495175627088	3.7622405582551552
7	3.764602576808733	3.732952730005195
8	3.4077308281843903	3.861807178435995
9	2.6218594428152873	3.34262171620006
>10	16.263992813656447	39.94801900541295
>50	0.3971256099430766	3.5812452601070874
>100	0.05325332042743877	1.6048612327688097
>500	0.011060378977730892	1.1337149709239407
>1k	0.02875698534093303	10.675032590756897
>5k	0.0066362273863691595	7.015683936785046
>10k+	0.0022120757954563865	3.702278159503119
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	11815	3.584680685807213	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	8912	2.7039081059597025	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	8317	2.5233846181852386	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5160	1.5655482301113177	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4652	1.4114206136584981	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	4110	1.2469773693328519	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	3889	1.1799257881594796	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3688	1.1189422233818875	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2551	0.7739754912817775	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2450	0.7433320084830869	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2377	0.7211837486384889	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2331	0.7072273109281941	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	2311	0.7011592945324138	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	1746	0.5297378313516202	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1478	0.44842641164816427	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	1465	0.44448220099090713	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1019	0.3091654353650064	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT	911	0.27639814682779273	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	769	0.2333152304177526	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	753	0.22846081730112833	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	674	0.20449215253779615	No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC	511	0.1550378189121867	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	497	0.1507902074351405	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	491	0.1489698025164064	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	486	0.14745279841746134	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	416	0.12621474103223027	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	6.068016395780301E-4	0.0	0.0
9	9.102024593670452E-4	0.0	0.0
10-14	0.0020631255745653023	0.0	0.0
15-19	0.002851967706016742	0.0	0.0
20-24	0.0035801296735103773	0.0	0.0
25-29	0.004672372624750831	0.0	0.0
30-34	0.011650591479898179	0.0	0.0
35-39	0.016201603776733402	0.0	0.0
40-44	0.02245166066438712	0.0	0.0
45-49	0.033313410012833854	0.0	0.0
50-54	0.04666304608355051	0.0	0.0
55-59	0.06941810756772665	0.0	0.0
60-64	0.08756147659110976	0.0	0.0
65-69	0.11741611725834881	0.0	0.0
70-74	0.1589213494054861	0.0	0.0
75-79	0.21420097877104466	0.0	0.0
80-84	0.2811918797804592	0.0	0.0
85-89	0.37227280588112144	0.0	0.0
90-94	0.4829534249401542	0.0	0.0
95-99	0.6193624335172954	0.0	0.0
100-104	0.7755531755446803	0.0	0.0
105-109	0.9707612629969326	0.0	0.0
110-114	1.1981298373468205	0.0	0.0
115-119	1.4562025746593568	0.0	0.0
120-124	1.747467361656811	0.0	0.0
125-129	2.096499664742094	0.0	0.0
130-134	2.505848050801433	0.0	0.0
135-139	2.91719888227138	0.0	0.0
140-144	3.3788535696623456	0.0	0.0
145-149	3.9289192559398303	0.0	0.0
150-154	4.595248136360465	0.0	0.0
155-159	5.345679724026614	0.0	0.0
160-164	6.150784139418744	0.0	0.0
165-169	7.0049181272887795	0.0	0.0
170-174	7.964574920281436	0.0	0.0
175-179	8.985154597887723	0.0	0.0
180-184	10.063077030434137	0.0	0.0
185-189	11.227650737112292	0.0	0.0
190-194	12.462734794309412	0.0	0.0
195-199	13.674639028874656	0.0	0.0
200-204	14.838666614077193	0.0	0.0
205-209	16.00336168108326	0.0	0.0
210-214	17.18795984186749	0.0	0.0
215-219	18.362303054942856	0.0	0.0
220-224	19.512010121451347	0.0	0.0
225-229	20.583197055798443	0.0	0.0
230-234	21.49619080270755	0.0	0.0
235-239	22.29462040006432	0.0	0.0
240-244	22.944201555232603	0.0	0.0
245-249	23.47211898166549	2.4272065583121204E-4	0.0
250-254	23.938142640861418	3.0340081978901505E-4	0.0
255-259	24.30550035346196	3.0340081978901505E-4	0.0
260-264	24.604350160954134	3.0340081978901505E-4	0.0
265-269	24.831172613828404	3.0340081978901505E-4	0.0
270-274	24.976865687491088	3.0340081978901505E-4	0.0
275-279	25.08123556949851	3.0340081978901505E-4	0.0
280-284	25.149804154770823	3.0340081978901505E-4	0.0
285-289	25.20192841561058	3.0340081978901505E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4705	0.0	291.23804	1
GGGTTAT	4730	0.0	290.32242	2
GTTATTA	4815	0.0	283.66565	4
TTATTAG	4790	0.0	283.60648	5
GGTTATT	4865	0.0	281.65982	3
TTAGTAG	5065	0.0	274.615	8
TAGTAGA	5135	0.0	270.58423	9
TATTAGT	5040	0.0	270.124	6
GGGGAGC	3840	0.0	268.4961	3
ATTAGTA	5095	0.0	266.91855	7
GGGGGGA	3920	0.0	266.0268	1
GGAGCGA	2865	0.0	262.0506	5
GGGAGCG	2910	0.0	259.01202	4
CGAAAGC	3080	0.0	249.02596	9
GAGCGAA	3165	0.0	237.2117	6
AGCGAAA	3230	0.0	231.98141	7
GCGAAAG	3330	0.0	229.8874	8
GGGGGAG	4625	0.0	224.2	2
GGGAGCA	1245	0.0	202.59036	4
GCAAAAG	1600	0.0	145.65625	8
>>END_MODULE