FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n02_7331_flu.3520000003bab9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n02_7331_flu.3520000003bab9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505680
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA224774.444905869324474No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC180863.576570162948901No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG99351.9646812213257396No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC97891.9358092074038917No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA85211.6850577440278438No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG84731.6755655750672362No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG74301.4693086536940358No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA48720.9634551495016611No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG45980.9092706850181933No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC44890.8877155513368138No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG42430.8390681854137003No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA38590.7631308337288404No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC30110.5954358487581078No Hit
GGGGGGAGCGAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT29290.5792200601170701No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT26030.5147524125929442No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG23390.4625454833096029No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA21830.4316959341876285No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC21370.4225992722670464No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA20290.40124189210567945No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG20260.40064863154564156No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT14610.2889178927384908Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC12100.23928175921531405No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT11520.22781205505457996No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA9700.19182091441227653No Hit
GGGGGGAGCAAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT9030.17857142857142858No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA7980.15780730897009967No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTCAACTGTCATACTCCT7420.14673311184939092No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG6270.12399145704793545No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT5450.10777566840689765No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG5060.10006328112640406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA89800.0288.92261
GGGTTAT90950.0285.431552
ATTAGTA92100.0278.504337
GGTTATT93350.0276.987153
TTATTAG93350.0276.03915
TATTAGT93350.0275.407076
GGGGGGA71750.0271.770051
GTTATTA96150.0268.307344
TAGTAGA96800.0266.35339
TTAGTAG97450.0265.33358
GGAGCGA54300.0264.848055
GCGAAAG55450.0261.48338
GAGCGAA55050.0260.168036
CGAAAGC55850.0259.346479
GGGGGAG76350.0258.294042
GGGGAGC81350.0241.874623
GGGAGCG61250.0234.79594
AGCGAAA62650.0228.371927
GAGCAAA28950.0166.096716
GCAAAAG30550.0158.363338
Produced by FastQC (version 0.11.2)