##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n02_7331_flu.3520000003bab9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505680
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.102993988292994	34.0	34.0	34.0	34.0	34.0
2	33.34354730264199	34.0	34.0	34.0	34.0	34.0
3	33.35238688498655	34.0	34.0	34.0	34.0	34.0
4	33.450199731055214	34.0	34.0	34.0	34.0	34.0
5	33.36538720139218	34.0	34.0	34.0	34.0	34.0
6	36.92674814111691	38.0	38.0	38.0	36.0	38.0
7	36.14928808732795	38.0	38.0	38.0	34.0	38.0
8	36.84625850340136	38.0	38.0	38.0	35.0	38.0
9	36.893834045246	38.0	38.0	38.0	36.0	38.0
10-14	36.90177701313083	38.0	38.0	38.0	36.0	38.0
15-19	36.790888308811894	38.0	38.0	38.0	35.8	38.0
20-24	36.883598718557195	38.0	38.0	38.0	36.0	38.0
25-29	36.798622053472556	38.0	38.0	38.0	36.0	38.0
30-34	36.43001661129568	38.0	37.6	38.0	33.8	38.0
35-39	36.721401676949846	38.0	38.0	38.0	35.6	38.0
40-44	36.22133325423193	38.0	37.2	38.0	33.2	38.0
45-49	36.55586220534725	38.0	38.0	38.0	34.2	38.0
50-54	36.482928334124345	38.0	37.6	38.0	34.2	38.0
55-59	36.118065575067234	38.0	37.0	38.0	33.0	38.0
60-64	36.28749446290145	38.0	37.0	38.0	33.8	38.0
65-69	36.190554500870114	38.0	37.0	38.0	33.6	38.0
70-74	36.07083333333334	38.0	37.0	38.0	32.4	38.0
75-79	36.09917774086379	38.0	37.0	38.0	32.2	38.0
80-84	35.32691860465117	38.0	36.6	38.0	29.0	38.0
85-89	35.5412707641196	38.0	37.0	38.0	30.0	38.0
90-94	35.51841520329062	38.0	37.0	38.0	29.8	38.0
95-99	34.58851328903654	38.0	35.4	38.0	24.4	38.0
100-104	34.943357854769815	38.0	36.0	38.0	26.0	38.0
105-109	34.61966619205822	38.0	35.4	38.0	24.2	38.0
110-114	34.09598995412118	37.2	34.2	38.0	23.6	38.0
115-119	34.118059246954594	37.2	34.2	38.0	23.4	38.0
120-124	33.61547500395507	37.0	33.2	38.0	22.2	38.0
125-129	33.29677780414491	37.0	32.0	38.0	22.6	38.0
130-134	33.10992801771871	37.0	31.8	38.0	21.6	38.0
135-139	32.41622567631704	37.0	30.2	38.0	19.2	38.0
140-144	31.09503203607024	35.8	26.8	37.8	14.6	38.0
145-149	30.868398591994936	35.0	26.8	37.6	15.0	38.0
150-154	29.86886964087961	35.0	24.0	37.0	10.2	38.0
155-159	29.55080722986869	34.6	23.8	37.0	10.0	37.0
160-164	28.85306834361652	33.4	22.8	37.0	10.2	37.4
165-169	27.493543743078625	31.0	20.6	36.6	9.4	37.0
170-174	26.906264831514	29.6	20.0	35.8	9.2	37.0
175-179	26.351478800822655	28.8	19.8	35.4	9.0	37.0
180-184	25.19954121183357	26.8	17.2	35.0	8.6	36.6
185-189	24.518335310868533	26.0	16.2	35.0	8.4	36.2
190-194	23.516152112007592	24.6	15.2	34.0	8.0	36.0
195-199	22.67718596741022	23.2	13.6	33.0	8.0	36.0
200-204	21.786370036386646	22.4	11.0	31.0	8.0	35.4
205-209	20.927126245847177	21.2	9.0	29.4	8.0	35.0
210-214	19.64403021673786	19.2	9.0	27.6	8.0	34.4
215-219	19.214634155987977	18.6	9.0	26.6	8.0	34.0
220-224	18.502571982281285	17.8	9.0	25.8	8.0	33.0
225-229	17.63360504666983	16.0	9.0	25.0	8.0	31.6
230-234	16.85735405790223	15.2	8.8	23.8	7.6	30.8
235-239	16.10019023888625	14.0	8.6	22.6	7.0	28.8
240-244	15.253965353583293	13.0	8.0	20.8	7.0	27.8
245-249	14.54157490903338	12.2	8.0	19.8	7.0	26.4
250-254	13.967221167536781	11.6	8.0	18.6	7.0	25.2
255-259	13.334052760639139	10.8	8.0	17.6	7.0	24.2
260-264	12.905582581869956	10.2	7.8	16.6	7.0	22.8
265-269	12.384800268944788	9.0	7.0	15.4	7.0	22.0
270-274	11.885814744502452	8.6	7.0	14.0	7.0	21.0
275-279	11.557729789590253	8.0	7.0	13.8	7.0	20.4
280-284	11.387668485999049	8.0	7.0	13.2	7.0	19.6
285-289	11.112044771396933	8.0	7.0	13.0	7.0	19.0
290-294	10.784642066128777	8.0	7.0	13.0	7.0	18.6
295-299	10.588298133206772	8.0	7.0	13.0	7.0	18.0
300-301	10.351008542952064	8.0	7.0	12.5	7.0	16.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.13608521050269928
1101	2	8.897951336663823E-4
1101	3	0.169941201946898
1101	4	0.12088817476702474
1101	5	0.17059424944559964
1101	6	0.19931270197078987
1101	7	1.065699173958933
1101	8	0.2919932908558991
1101	9	0.027369431856214987
1101	10-14	0.099991459344416
1101	15-19	0.1864841597859126
1101	20-24	0.2050150418644634
1101	25-29	0.09346299680175463
1101	30-34	0.490197681729974
1101	35-39	0.2109469520279177
1101	40-44	0.5244584173829239
1101	45-49	0.21234077018554132
1101	50-54	0.25966316653352806
1101	55-59	0.38324388800146636
1101	60-64	0.2734006932916131
1101	65-69	0.371348149755228
1101	70-74	0.28156411227340783
1101	75-79	0.42595921944984383
1101	80-84	0.7931401112611596
1101	85-89	0.40588376116458136
1101	90-94	0.5212465978340148
1101	95-99	1.090814268078553
1101	100-104	0.5744794635028185
1101	105-109	0.6846356343897781
1101	110-114	0.6362007604031916
1101	115-119	0.5692147502112803
1101	120-124	0.9056245028733514
1101	125-129	0.8087816280331239
1101	130-134	0.8306579133111072
1101	135-139	0.822873790585561
1101	140-144	1.095120856424117
1101	145-149	1.6536266389839618
1101	150-154	1.3156039870277958
1101	155-159	0.8171915321691507
1101	160-164	1.2056589527734864
1101	165-169	0.7926968377188679
1101	170-174	0.6357138948997196
1101	175-179	0.9771748228017572
1101	180-184	0.7271336887973554
1101	185-189	0.7810007713992171
1101	190-194	0.8283167196957741
1101	195-199	0.96961776847888
1101	200-204	1.2288892010134802
1101	205-209	0.864664854212208
1101	210-214	1.0756118115769482
1101	215-219	1.0233023744887646
1101	220-224	0.6572378023329755
1101	225-229	0.4379144074716166
1101	230-234	0.4801848138684335
1101	235-239	0.2309217543383717
1101	240-244	0.26974724688700036
1101	245-249	0.27842586330718966
1101	250-254	0.4902977259116117
1101	255-259	0.2563382649189627
1101	260-264	-0.10704608711496277
1101	265-269	0.037700494901381276
1101	270-274	0.1886469282905221
1101	275-279	0.10456221917519137
1101	280-284	-0.03240527411450422
1101	285-289	-0.04925699045037568
1101	290-294	-0.04677199591457004
1101	295-299	0.07407288878181895
1101	300-301	-0.18528219571993176
1102	1	-0.08777831296814753
1102	2	0.09291872970089798
1102	3	5.051541145775218E-4
1102	4	0.07432527299082636
1102	5	0.07500165261409109
1102	6	-0.04295126350107381
1102	7	0.892805781142151
1102	8	0.1374393383222099
1102	9	0.08987643979166648
1102	10-14	-0.01578747740145303
1102	15-19	0.06438776893803322
1102	20-24	0.06784448745180782
1102	25-29	0.010509374955461226
1102	30-34	0.37156293503525006
1102	35-39	0.06171502175346433
1102	40-44	0.3336322624025314
1102	45-49	-0.014396573265173629
1102	50-54	-0.0632220636724341
1102	55-59	0.21783709078336955
1102	60-64	0.08806143949949075
1102	65-69	-0.030485039013711912
1102	70-74	-0.09164974290153793
1102	75-79	0.1265878857257121
1102	80-84	0.5810839641180436
1102	85-89	0.22303883856994844
1102	90-94	0.049625352271895906
1102	95-99	0.7020456719953287
1102	100-104	0.5457457952603733
1102	105-109	0.5663278569031291
1102	110-114	0.5586934881269627
1102	115-119	0.47063887888887734
1102	120-124	0.7864819894980002
1102	125-129	0.6863755808456702
1102	130-134	0.7251661900822981
1102	135-139	0.6416655731312773
1102	140-144	1.3346363749706711
1102	145-149	1.4993010071659363
1102	150-154	1.434538165012217
1102	155-159	1.032578373110944
1102	160-164	1.2553273648639127
1102	165-169	1.175019120833067
1102	170-174	1.1959715661562313
1102	175-179	1.2688340288614945
1102	180-184	1.032907530447357
1102	185-189	1.0450084215956146
1102	190-194	1.0964031190432308
1102	195-199	1.2055404601302975
1102	200-204	1.0521187713929088
1102	205-209	0.9663413557113998
1102	210-214	1.1959677362671322
1102	215-219	1.229664214602682
1102	220-224	0.928703929240946
1102	225-229	0.7043221557355395
1102	230-234	0.7637787794622994
1102	235-239	0.5229344217808212
1102	240-244	0.5310221363141263
1102	245-249	0.5642630654082055
1102	250-254	0.5738041976129171
1102	255-259	0.5183845646419023
1102	260-264	0.31076316109405866
1102	265-269	0.2338925933779077
1102	270-274	0.1585463567915717
1102	275-279	0.2011668635555104
1102	280-284	0.1265207005912785
1102	285-289	0.08143378719371519
1102	290-294	-0.03064546557027903
1102	295-299	0.021728534665102828
1102	300-301	-0.07919149371796941
1103	1	-0.16133646235933696
1103	2	0.014888122949543003
1103	3	0.0708835002961834
1103	4	0.08290134886625111
1103	5	-0.07639638406361371
1103	6	0.0721041195987766
1103	7	0.9717241211382586
1103	8	0.12426079512110277
1103	9	0.21217515343756332
1103	10-14	0.024217791724900906
1103	15-19	0.06731087405709957
1103	20-24	0.10473856655742964
1103	25-29	-0.03818365261581391
1103	30-34	0.47634363628723975
1103	35-39	0.20834060450739145
1103	40-44	0.45513350522907814
1103	45-49	0.14238788510299827
1103	50-54	0.052319453606862965
1103	55-59	0.32430425610794344
1103	60-64	0.0830376711577756
1103	65-69	0.2958416926388452
1103	70-74	0.2874868076534369
1103	75-79	0.31577414934238845
1103	80-84	0.8299839767181325
1103	85-89	0.4539970177431485
1103	90-94	0.42458520381998
1103	95-99	1.160737325011901
1103	100-104	0.7123218940450329
1103	105-109	0.7954211089850105
1103	110-114	0.6365344063295026
1103	115-119	0.8363226625797893
1103	120-124	0.9913795278996176
1103	125-129	0.9448930090538212
1103	130-134	0.9767051186169056
1103	135-139	0.8984447623731029
1103	140-144	1.613740852582616
1103	145-149	1.830311533964025
1103	150-154	1.9615636286380642
1103	155-159	0.9022275180233734
1103	160-164	1.003119944559451
1103	165-169	1.6789177016053394
1103	170-174	1.7860165828227075
1103	175-179	1.5825808358854658
1103	180-184	1.1378947585432506
1103	185-189	1.2738930850015358
1103	190-194	1.3500325161864488
1103	195-199	1.2552339344318533
1103	200-204	1.3071937418972546
1103	205-209	0.917135619236447
1103	210-214	1.5087854588590837
1103	215-219	1.3326099998743288
1103	220-224	1.1092574166007694
1103	225-229	0.5728540167680549
1103	230-234	1.2913483376575101
1103	235-239	0.6305918369307051
1103	240-244	0.5142587544513759
1103	245-249	0.9062752989450473
1103	250-254	0.7533669746715965
1103	255-259	0.6957047557050018
1103	260-264	0.38100932093186124
1103	265-269	0.414942294984991
1103	270-274	0.32830695518105024
1103	275-279	0.3816417722048939
1103	280-284	0.2611465353468958
1103	285-289	0.30854103682430356
1103	290-294	-0.042544985621596254
1103	295-299	0.057003617424749464
1103	300-301	-0.19417471269598963
2116	1	-0.05372630953066704
2116	2	-0.13154140605215758
2116	3	-0.019944545105552436
2116	4	0.011152309631285107
2116	5	0.07338688521871717
2116	6	0.04291920584419273
2116	7	0.8238579132555159
2116	8	0.20727759733756557
2116	9	0.2566317626072845
2116	10-14	0.05916059269013374
2116	15-19	0.12244448742738001
2116	20-24	0.1008566289401287
2116	25-29	0.11455117623395239
2116	30-34	0.46221681530234804
2116	35-39	0.0027690610271804417
2116	40-44	0.39696744928127004
2116	45-49	0.2057282193598624
2116	50-54	0.1698424672810006
2116	55-59	0.26156540545105145
2116	60-64	0.37253807333848954
2116	65-69	0.43458957001841014
2116	70-74	0.3631369998064429
2116	75-79	0.42271623531969027
2116	80-84	0.8188319524085443
2116	85-89	0.5194858907434252
2116	90-94	0.5290824779284762
2116	95-99	1.0860023174151578
2116	100-104	0.5129774486075505
2116	105-109	0.4921674670152143
2116	110-114	0.608376393995961
2116	115-119	0.7614330388421848
2116	120-124	1.1007110141807885
2116	125-129	0.8147790578517586
2116	130-134	1.0365808894632806
2116	135-139	1.4869919962635336
2116	140-144	1.8677202073060002
2116	145-149	1.8893092102532343
2116	150-154	2.0173684369319247
2116	155-159	1.880933389861788
2116	160-164	1.6752211153521905
2116	165-169	1.530517487108959
2116	170-174	1.689097287085822
2116	175-179	1.9828185693737197
2116	180-184	1.7003726386040263
2116	185-189	1.1740883660811647
2116	190-194	1.3180144627411003
2116	195-199	0.6302419780461186
2116	200-204	0.5828886207961723
2116	205-209	0.854133116778673
2116	210-214	1.723176825379774
2116	215-219	1.5739202152644438
2116	220-224	1.1853812882604373
2116	225-229	1.0261072118703893
2116	230-234	1.1231720037581425
2116	235-239	0.7015476864257444
2116	240-244	0.6154800432171257
2116	245-249	0.8117056323081737
2116	250-254	0.83896979887394
2116	255-259	1.1411896068981395
2116	260-264	0.38516608203651614
2116	265-269	0.6036696164614419
2116	270-274	0.5100921906802007
2116	275-279	0.43752388856918145
2116	280-284	0.12957043381860878
2116	285-289	0.09317683984512648
2116	290-294	0.10139861970506203
2116	295-299	0.21886124545227936
2116	300-301	-0.11806424706009722
1117	1	0.22798360397565887
1117	2	0.11827673544263462
1117	3	0.2552284422530633
1117	4	0.15745082371422825
1117	5	0.16376945341566085
1117	6	0.16616160992492013
1117	7	0.9568153317518551
1117	8	0.44220441037814595
1117	9	0.3084825055658058
1117	10-14	0.1836185656331466
1117	15-19	0.25305437875583436
1117	20-24	0.37271313814394347
1117	25-29	0.32416128934107036
1117	30-34	0.5524055489461688
1117	35-39	0.3366435343028513
1117	40-44	0.699633228833278
1117	45-49	0.3367162433119546
1117	50-54	0.4000773308871217
1117	55-59	0.46945074588130353
1117	60-64	0.5644218613180811
1117	65-69	0.5055321293442034
1117	70-74	0.4609152211415548
1117	75-79	0.6578581736652183
1117	80-84	1.0414267627877862
1117	85-89	0.6926393615795305
1117	90-94	0.5855326886182155
1117	95-99	1.1420817141771877
1117	100-104	0.46766296646073613
1117	105-109	0.5575449343028609
1117	110-114	0.9898290442510032
1117	115-119	0.9830866205676188
1117	120-124	1.088440887766943
1117	125-129	1.0225082661019158
1117	130-134	1.0949383966710826
1117	135-139	1.1518908651923425
1117	140-144	1.253599337933636
1117	145-149	1.998420986700335
1117	150-154	2.3059428436739964
1117	155-159	1.8584663239935217
1117	160-164	1.9198474183018455
1117	165-169	1.370144898951942
1117	170-174	1.1183946922754444
1117	175-179	1.3262148878478754
1117	180-184	1.370134670096597
1117	185-189	1.1885951303304516
1117	190-194	-0.7295672626969143
1117	195-199	1.134695515415821
1117	200-204	1.4409560415102973
1117	205-209	1.4239714401319645
1117	210-214	1.8993283002078947
1117	215-219	1.4737388003165606
1117	220-224	1.5846250230131531
1117	225-229	1.4588794451815446
1117	230-234	1.0617994156011257
1117	235-239	0.9324492166985365
1117	240-244	0.9123483066901805
1117	245-249	0.738692103809619
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2108	255-259	-0.5232537246206395
2108	260-264	-0.34254888802336225
2108	265-269	-0.4375830781493377
2108	270-274	-0.31719806517173055
2108	275-279	-0.1841850934667466
2108	280-284	-0.7060355542053465
2108	285-289	-0.9729444448581948
2108	290-294	-0.6610546192715301
2108	295-299	-0.49769868922382443
2108	300-301	2.6912934012795855
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	113.0
11	581.0
12	987.0
13	1046.0
14	1339.0
15	2049.0
16	3062.0
17	4599.0
18	5762.0
19	6820.0
20	8799.0
21	11969.0
22	15602.0
23	21522.0
24	30050.0
25	41851.0
26	56439.0
27	71050.0
28	79539.0
29	71596.0
30	46764.0
31	19499.0
32	4233.0
33	392.0
34	12.0
35	1.0
36	0.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.0390365448505	18.872607182407847	13.87280493592786	35.215551336813796
2	49.96044929599746	22.370866951431736	14.123951906343931	13.544731846226862
3	49.43640246796393	21.631466540104412	16.291330485682646	12.640800506249011
4	50.072971048884675	20.15622528081	16.57372251226072	13.197081158044613
5	30.804065812371462	20.542042398354692	35.80525233349154	12.848639455782312
6	29.683989875019773	19.924260401835152	36.02713178294574	14.364617940199336
7	16.202736908716975	51.695934187628545	18.111058376839107	13.990270526815376
8	28.857973421926907	18.513486790064864	37.667299477930705	14.96124031007752
9	14.812727416548013	17.434939091915837	38.52416548014554	29.228168011390604
10-14	27.534962822338237	35.393806359753206	22.158835627274165	14.912395190634392
15-19	21.941820914412276	36.68185413700364	19.528001898433793	21.84832305015029
20-24	27.461279860781524	30.028436956177817	25.328112640405	17.182170542635657
25-29	18.837288403733588	26.16686442018668	37.66907925961082	17.326767916468913
30-34	19.351763961398515	28.21278278753362	30.33376839107736	22.10168485999051
35-39	20.81624742920424	30.059721563043823	28.277566840689765	20.846464167062173
40-44	20.598995412118335	30.58887043189369	27.90891472868217	20.903219427305807
45-49	21.76115329852871	28.783736750514162	26.23868849865528	23.21642145230185
50-54	23.109792754311027	30.633681379528554	24.69747666508464	21.559049201075776
55-59	22.510204081632654	27.346266413542157	27.53796867584243	22.605560828982757
60-64	20.828547698149027	27.1218557190318	28.861493434583135	23.18810314823604
65-69	18.069174181300426	29.035239677266254	28.50549754785635	24.390088593576966
70-74	20.431142224331595	26.177899066603384	30.56296472077203	22.82799398829299
75-79	21.395071982281287	25.166745768074673	27.67627748773928	25.761904761904763
80-84	23.576174655908876	27.534488213890207	24.717212466381902	24.172124663819016
85-89	21.80505457997152	27.587169751621577	25.80683436165164	24.800941306755263
90-94	22.800585350419237	29.213376048093654	27.663344407530456	20.322694193956654
95-99	23.88348362600854	27.32700522069293	27.392659389337133	21.3968517639614
100-104	22.791963296946687	26.662236987818382	27.372884037335865	23.172915677899066
105-109	25.08558772346148	27.949770605916786	24.32277329536466	22.64186837525708
110-114	20.617386489479514	27.051455465907292	30.32676791646891	22.00439012814428
115-119	25.66583610188261	25.721167536782154	26.906502135738013	21.706494225597215
120-124	20.337683910773613	27.100340136054424	26.89329220060117	25.668683752570796
125-129	19.83875177978168	24.151320993513682	30.769973105521274	25.239954121183356
130-134	24.21468122132574	27.941781363708273	26.5609080841639	21.282629330802088
135-139	23.472591362126245	25.415559246954594	25.53057269419396	25.5812766967252
140-144	21.50980857459263	26.769063439329223	27.038838791330488	24.682289194747668
145-149	22.93545325106787	24.251779781680114	29.25794969150451	23.55481727574751
150-154	21.527725043505775	26.865211200759376	25.55798133206771	26.04908242366714
155-159	21.562569213732004	25.71543268470179	30.85738016136687	21.864617940199334
160-164	23.308851447555767	29.494621104255657	24.46468122132574	22.731846226862835
165-169	21.613391868375256	28.30841638981174	26.301969625059325	23.77622211675368
170-174	21.80066445182724	29.851645309286507	26.094447081158044	22.253243157728207
175-179	19.753678215472238	27.68402942572378	28.085389969941467	24.476902388862523
180-184	21.4875019775352	28.135619364024677	27.24952539155197	23.12735326688815
185-189	20.782550229394083	26.365804461319414	28.838474924853664	24.013170384432843
190-194	20.812925170068027	24.96938775510204	27.675526024363233	26.5421610504667
195-199	19.50735643094447	30.718438538205977	26.898868849865526	22.87533618098402
200-204	21.371460211991774	26.64345040341718	24.968161683277962	27.016927701313083
205-209	21.976032273374464	25.520210409745292	28.085073564309443	24.418683752570796
210-214	23.68339661445974	25.055647840531563	27.991892105679483	23.269063439329223
215-219	23.04560196171492	26.212189526973578	28.60647049517481	22.13573801613669
220-224	21.142778041449137	26.042081949058694	27.31767125454833	25.497468754943835
225-229	24.626522702104097	25.92548647365923	26.52772504350577	22.920265780730897
230-234	23.744937509887677	25.889732637240943	27.26807467172916	23.097255181142224
235-239	23.007277329536464	26.226546432526497	25.167576332858726	25.598599905078313
240-244	23.685651004587882	27.940238886252178	25.020922322417338	23.353187786742605
245-249	24.096068660022148	26.459500079101407	24.644755576649263	24.79967568422718
250-254	22.228168011390604	26.17916468913147	23.93311975953172	27.65954753994621
255-259	22.92406264831514	27.14621895269736	24.050980857459262	25.878737541528242
260-264	21.62138111058377	26.33345198544534	25.873912355639934	26.171254548330957
265-269	22.302997943363394	27.022029742129412	24.26748141116912	26.407490903338076
270-274	21.515859832305015	28.625968992248062	23.633206771080527	26.224964404366396
275-279	21.98485208036703	27.517916468913146	23.94205821863629	26.55517323208353
280-284	20.021159626641357	28.35892263882297	26.236315456415127	25.38360227812055
285-289	21.140404999208986	28.384432842904605	24.091203923429838	26.38395823445657
290-294	21.846661920582186	28.769261192849232	23.47745609871856	25.90662078785002
295-299	20.638664768232875	29.041607340610664	22.912118335706374	27.407609555450087
300-301	16.733803986710964	29.045740389178924	28.692255972156307	25.528199651953802
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.25
20	0.0
21	0.0
22	0.0
23	0.0
24	0.25
25	0.5
26	0.5
27	1.25
28	1.25
29	2.25
30	4.0
31	8.5
32	35.0
33	100.5
34	223.5
35	434.75
36	817.5
37	1644.25
38	3262.5
39	5740.0
40	8396.25
41	9722.25
42	10765.0
43	12809.25
44	14988.5
45	15352.0
46	14778.25
47	14248.0
48	12876.5
49	10872.0
50	8632.5
51	6123.5
52	3599.25
53	1868.0
54	1035.0
55	770.5
56	671.25
57	667.75
58	733.0
59	878.75
60	1106.0
61	1320.0
62	1350.75
63	1100.25
64	695.25
65	374.75
66	209.25
67	121.5
68	65.25
69	44.25
70	26.5
71	25.75
72	21.75
73	21.0
74	18.75
75	15.0
76	12.5
77	8.25
78	11.0
79	10.0
80	5.75
81	4.0
82	3.5
83	2.0
84	1.5
85	0.75
86	1.0
87	0.25
88	0.25
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	1.25
95	0.5
96	0.75
97	1.0
98	1.0
99	0.25
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	505680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.611872333690453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.67842235584301	8.123350661837932
2	5.69273497018747	1.5497756328745391
3	3.3538576185062916	1.3695684518544824
4	3.653173212471655	1.9890610952408798
5	3.2640416162765447	2.221485888630448
6	2.985395700897958	2.4382095087702798
7	2.4247835880634656	2.3104091246283422
8	1.9099096538632891	2.079795710181601
9	1.7038205359726788	2.0872968853662126
>10	14.222095431829333	36.728060674834815
>50	0.9148332156366713	8.200773180059835
>100	0.15188298723850552	3.319739255658212
>500	0.010511460058618154	1.0406051329986292
>1k	0.024026193498801855	9.226862115086844
>5k	0.00750818546837558	9.023891997449192
>10k+	0.003003274187350232	8.291114684527761
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	22477	4.444905869324474	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	18086	3.576570162948901	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	9935	1.9646812213257396	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	9789	1.9358092074038917	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	8521	1.6850577440278438	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	8473	1.6755655750672362	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	7430	1.4693086536940358	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	4872	0.9634551495016611	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	4598	0.9092706850181933	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	4489	0.8877155513368138	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	4243	0.8390681854137003	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	3859	0.7631308337288404	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	3011	0.5954358487581078	No Hit
GGGGGGAGCGAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT	2929	0.5792200601170701	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	2603	0.5147524125929442	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	2339	0.4625454833096029	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	2183	0.4316959341876285	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	2137	0.4225992722670464	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	2029	0.40124189210567945	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	2026	0.40064863154564156	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT	1461	0.2889178927384908	Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	1210	0.23928175921531405	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	1152	0.22781205505457996	No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA	970	0.19182091441227653	No Hit
GGGGGGAGCAAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT	903	0.17857142857142858	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	798	0.15780730897009967	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTCAACTGTCATACTCCT	742	0.14673311184939092	No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG	627	0.12399145704793545	No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT	545	0.10777566840689765	No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	506	0.10006328112640406	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	0.0010283183040658123	0.0	0.0
15-19	0.001779781680113906	0.0	0.0
20-24	0.0023334915361493433	0.0	0.0
25-29	0.0024916943521594683	0.0	0.0
30-34	0.006802721088435373	0.0	0.0
35-39	0.007712387280493593	0.0	0.0
40-44	0.008701154880556875	0.0	0.0
45-49	0.009769023888625217	0.0	0.0
50-54	0.011588356272741653	0.0	0.0
55-59	0.01716500553709856	0.0	0.0
60-64	0.025747508305647843	0.0	0.0
65-69	0.036742604018351534	0.0	0.0
70-74	0.05299794336339188	0.0	0.0
75-79	0.07649106154089542	0.0	0.0
80-84	0.10908084163898117	0.0	0.0
85-89	0.14511153298528714	0.0	0.0
90-94	0.19190001582028163	0.0	0.0
95-99	0.25482518588830877	0.0	0.0
100-104	0.33266097136529027	0.0	0.0
105-109	0.41943521594684385	0.0	0.0
110-114	0.5133681379528555	0.0	0.0
115-119	0.6178610979275432	0.0	0.0
120-124	0.7369087169751621	0.0	0.0
125-129	0.8865290302167377	0.0	0.0
130-134	1.0530770447713969	0.0	0.0
135-139	1.2206929283341243	0.0	0.0
140-144	1.4082423667141275	0.0	0.0
145-149	1.6516373991457047	0.0	0.0
150-154	1.9260797342192688	0.0	0.0
155-159	2.2542714760322733	0.0	0.0
160-164	2.613945578231293	0.0	0.0
165-169	3.0372567631703844	0.0	0.0
170-174	3.4905078310393924	0.0	0.0
175-179	3.9886093972472714	0.0	0.0
180-184	4.513447239360859	0.0	0.0
185-189	5.081316247429204	0.0	0.0
190-194	5.698979591836735	0.0	0.0
195-199	6.33467805726942	0.0	0.0
200-204	6.996915045087803	1.5820281601012498E-4	0.0
205-209	7.690120234140167	1.9775352001265623E-4	0.0
210-214	8.438973263724094	1.9775352001265623E-4	0.0
215-219	9.080406581237146	1.9775352001265623E-4	0.0
220-224	9.723501028318305	1.9775352001265623E-4	0.0
225-229	10.315100458788166	1.9775352001265623E-4	0.0
230-234	10.884867900648633	1.9775352001265623E-4	0.0
235-239	11.367386489479513	1.9775352001265623E-4	0.0
240-244	11.771792437905395	1.9775352001265623E-4	0.0
245-249	12.09045246005379	1.9775352001265623E-4	0.0
250-254	12.363668723303274	1.9775352001265623E-4	1.5820281601012498E-4
255-259	12.579338712229077	1.9775352001265623E-4	1.9775352001265623E-4
260-264	12.760995095712705	1.9775352001265623E-4	1.9775352001265623E-4
265-269	12.90393133997785	1.9775352001265623E-4	1.9775352001265623E-4
270-274	13.014871064704952	1.9775352001265623E-4	1.9775352001265623E-4
275-279	13.086853345989558	1.9775352001265623E-4	1.9775352001265623E-4
280-284	13.138387913304857	1.9775352001265623E-4	1.9775352001265623E-4
285-289	13.17845277645942	1.9775352001265623E-4	1.9775352001265623E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	8980	0.0	288.9226	1
GGGTTAT	9095	0.0	285.43155	2
ATTAGTA	9210	0.0	278.50433	7
GGTTATT	9335	0.0	276.98715	3
TTATTAG	9335	0.0	276.0391	5
TATTAGT	9335	0.0	275.40707	6
GGGGGGA	7175	0.0	271.77005	1
GTTATTA	9615	0.0	268.30734	4
TAGTAGA	9680	0.0	266.3533	9
TTAGTAG	9745	0.0	265.3335	8
GGAGCGA	5430	0.0	264.84805	5
GCGAAAG	5545	0.0	261.4833	8
GAGCGAA	5505	0.0	260.16803	6
CGAAAGC	5585	0.0	259.34647	9
GGGGGAG	7635	0.0	258.29404	2
GGGGAGC	8135	0.0	241.87462	3
GGGAGCG	6125	0.0	234.7959	4
AGCGAAA	6265	0.0	228.37192	7
GAGCAAA	2895	0.0	166.09671	6
GCAAAAG	3055	0.0	158.36333	8
>>END_MODULE