##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJTU1_l01n02_7153_flu.3520000003ba26.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 279927 Sequences flagged as poor quality 0 Sequence length 301 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.103973535957586 34.0 34.0 34.0 34.0 34.0 2 33.44157583941528 34.0 34.0 34.0 34.0 34.0 3 33.428422410128356 34.0 34.0 34.0 34.0 34.0 4 33.451614170837395 34.0 34.0 34.0 34.0 34.0 5 33.459630546535344 34.0 34.0 34.0 34.0 34.0 6 36.9756543670314 38.0 38.0 38.0 36.0 38.0 7 36.12724746094518 38.0 38.0 38.0 34.0 38.0 8 36.90019898044847 38.0 38.0 38.0 36.0 38.0 9 37.01358211247933 38.0 38.0 38.0 36.0 38.0 10-14 36.99368978340782 38.0 38.0 38.0 36.4 38.0 15-19 36.91743418819907 38.0 38.0 38.0 36.6 38.0 20-24 37.030314331950834 38.0 38.0 38.0 36.8 38.0 25-29 36.941879847245886 38.0 38.0 38.0 36.0 38.0 30-34 36.58831838300701 38.0 38.0 38.0 34.6 38.0 35-39 36.88805795796761 38.0 38.0 38.0 36.0 38.0 40-44 36.40249350723582 38.0 37.6 38.0 33.8 38.0 45-49 36.72377012578279 38.0 38.0 38.0 35.0 38.0 50-54 36.68841805184923 38.0 38.0 38.0 34.8 38.0 55-59 36.337664462520586 38.0 37.4 38.0 33.8 38.0 60-64 36.52420595369507 38.0 37.4 38.0 34.2 38.0 65-69 36.39171641177879 38.0 37.0 38.0 34.0 38.0 70-74 36.25724135220968 38.0 37.0 38.0 34.0 38.0 75-79 36.33420284574193 38.0 37.0 38.0 34.0 38.0 80-84 35.6000335801834 38.0 36.8 38.0 30.0 38.0 85-89 35.834438264261756 38.0 37.0 38.0 31.4 38.0 90-94 35.81526183612156 38.0 37.0 38.0 31.2 38.0 95-99 34.929903153322115 38.0 36.0 38.0 26.8 38.0 100-104 35.24028050170223 38.0 36.0 38.0 28.2 38.0 105-109 35.00679105623966 38.0 35.8 38.0 27.2 38.0 110-114 34.58694659679131 38.0 35.0 38.0 24.0 38.0 115-119 34.619243588507004 38.0 35.0 38.0 24.4 38.0 120-124 34.127070271892315 37.6 34.6 38.0 23.2 38.0 125-129 33.63408745851597 37.0 34.0 38.0 22.8 38.0 130-134 33.504913781092924 37.0 33.6 38.0 22.4 38.0 135-139 32.77526855215824 37.0 30.8 38.0 20.8 38.0 140-144 31.56454575657225 36.0 27.4 38.0 17.0 38.0 145-149 31.34716408206425 35.0 27.8 37.6 16.8 38.0 150-154 30.28022948840233 35.0 25.0 37.0 12.4 38.0 155-159 30.172641438660794 35.0 25.0 37.0 13.0 37.4 160-164 29.340751695977882 34.0 23.4 37.0 10.6 37.4 165-169 28.068308523293574 32.0 21.6 36.6 9.6 37.0 170-174 27.47785672693238 31.2 20.4 36.2 9.2 37.0 175-179 26.697814072954735 29.4 20.0 35.4 9.0 37.0 180-184 25.73155286914088 27.6 18.8 35.0 8.8 37.0 185-189 24.99272524622491 26.4 17.2 35.0 8.4 36.6 190-194 24.011980980755695 25.2 15.8 34.2 8.0 36.0 195-199 23.171557584656 24.2 14.6 33.0 8.0 36.0 200-204 22.32133091841802 23.0 12.8 31.4 8.0 36.0 205-209 21.363088233718074 21.8 10.4 30.4 8.0 35.2 210-214 20.055926009280988 20.2 9.0 28.2 8.0 34.4 215-219 19.641264329628797 19.4 9.0 27.6 8.0 34.0 220-224 18.959817380960036 18.6 9.0 26.2 8.0 33.2 225-229 18.20223915520832 16.8 9.0 25.4 7.4 32.4 230-234 17.333807742732926 15.8 9.0 24.6 7.0 31.4 235-239 16.362465928617105 14.6 8.2 22.8 7.0 29.4 240-244 15.606214477345882 13.4 8.0 21.4 7.0 28.8 245-249 14.855382296098625 12.8 8.0 20.4 7.0 26.8 250-254 14.189796625548803 11.8 8.0 19.2 7.0 25.6 255-259 13.570057907954574 11.2 8.0 18.2 7.0 24.4 260-264 13.026444037195409 10.6 7.8 17.2 7.0 23.2 265-269 12.558313417426687 9.8 7.2 16.0 7.0 22.4 270-274 12.139381338706162 9.0 7.4 14.6 7.0 21.4 275-279 11.730744801323201 8.0 7.0 14.0 7.0 20.8 280-284 11.552656228230932 8.0 7.0 13.4 7.0 20.2 285-289 11.204516177431973 8.0 7.0 13.0 7.0 19.0 290-294 10.988805652902363 8.0 7.0 13.0 7.0 19.0 295-299 10.723782271806579 8.0 7.0 13.0 7.0 18.4 300-301 10.460234275364648 8.0 7.0 12.5 7.0 16.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.18914380499167294 1101 2 0.06668260624444855 1101 3 0.14342049439737536 1101 4 0.2214514768767586 1101 5 0.11938207593595251 1101 6 0.1607544391296969 1101 7 0.9468153969357971 1101 8 0.34516839034547075 1101 9 0.14292122798107698 1101 10-14 0.14434990211400844 1101 15-19 0.21167152944037326 1101 20-24 0.20306366135663012 1101 25-29 0.1232718129335737 1101 30-34 0.4834282783043662 1101 35-39 0.11033708749709348 1101 40-44 0.5431394775614606 1101 45-49 0.19648522417463 1101 50-54 0.212960900159878 1101 55-59 0.3591222494172115 1101 60-64 0.32931766003705576 1101 65-69 0.26415633926686155 1101 70-74 0.3233032672093472 1101 75-79 0.20421930880943506 1101 80-84 0.8148435152219164 1101 85-89 0.36147544492872896 1101 90-94 0.43332958197437677 1101 95-99 0.9966076480858348 1101 100-104 0.5904932285146813 1101 105-109 0.5720115289371961 1101 110-114 0.5577484078937047 1101 115-119 0.548871161704902 1101 120-124 1.0979612658564406 1101 125-129 0.6654581925311192 1101 130-134 0.6908223746830302 1101 135-139 0.48623958373428877 1101 140-144 1.1968532211563918 1101 145-149 1.2802568181322833 1101 150-154 0.9061718573715218 1101 155-159 0.9958740000181905 1101 160-164 1.0520899343882846 1101 165-169 0.5514129067670694 1101 170-174 0.44607777551349415 1101 175-179 0.7330544891143269 1101 180-184 0.4893068028337666 1101 185-189 -0.0508789320819254 1101 190-194 0.5740881038754821 1101 195-199 0.631736185320193 1101 200-204 0.6590371707258242 1101 205-209 0.3078407966023633 1101 210-214 0.8437316390963936 1101 215-219 0.497455276556952 1101 220-224 0.4403684590890258 1101 225-229 0.21504570448851723 1101 230-234 0.25873194669144084 1101 235-239 0.062193203432414634 1101 240-244 0.17385844152913066 1101 245-249 -0.21976997736941684 1101 250-254 -0.23675536049415413 1101 255-259 -0.11734835688733725 1101 260-264 -0.18311585025986687 1101 265-269 -0.1255186651327147 1101 270-274 -0.02465406962781813 1101 275-279 0.040262853982588 1101 280-284 0.3525285479547975 1101 285-289 0.17965191604694297 1101 290-294 -0.11312838064916875 1101 295-299 -0.06680754297590319 1101 300-301 -0.38621230235574977 1102 1 -0.19892336922712417 1102 2 -0.07466760472601663 1102 3 0.04772513269004719 1102 4 -0.09760121987613246 1102 5 -0.08132077675163174 1102 6 -0.2693539507681848 1102 7 0.8963793148713464 1102 8 0.09123562571512167 1102 9 0.11173046787590124 1102 10-14 0.039179596971230524 1102 15-19 0.08205616834454332 1102 20-24 0.07682229891548786 1102 25-29 -0.023553693089780836 1102 30-34 0.30608084969799165 1102 35-39 0.18810798580321375 1102 40-44 0.3469296842513714 1102 45-49 0.020991430986839532 1102 50-54 0.03009919282789042 1102 55-59 0.13125771385798402 1102 60-64 0.11721284746444383 1102 65-69 0.05608824778517629 1102 70-74 -0.018169627672321553 1102 75-79 0.10425982125363475 1102 80-84 0.5918442948953668 1102 85-89 0.06785653708306683 1102 90-94 0.3169973340688017 1102 95-99 0.7921669491315058 1102 100-104 0.5847740944108466 1102 105-109 0.7543771499241672 1102 110-114 0.5323956438748709 1102 115-119 0.5621481598092117 1102 120-124 0.846096164651776 1102 125-129 0.6176187288241621 1102 130-134 0.8853814769884139 1102 135-139 0.7604251307287271 1102 140-144 1.4672061380523793 1102 145-149 1.4374844674932596 1102 150-154 1.3391982898303958 1102 155-159 0.7543774091785913 1102 160-164 1.2892237936190085 1102 165-169 1.4148962890210903 1102 170-174 1.2625208593513229 1102 175-179 1.2936427603795373 1102 180-184 1.1571529166966563 1102 185-189 1.1159073609529884 1102 190-194 0.8708077424739038 1102 195-199 1.4078709159506602 1102 200-204 1.7388428638708078 1102 205-209 1.2227142143238794 1102 210-214 0.9871972876012514 1102 215-219 1.214433048219643 1102 220-224 0.9912906904838756 1102 225-229 1.0335545407876658 1102 230-234 0.9922066913866026 1102 235-239 0.7914546539307956 1102 240-244 0.8423772148428945 1102 245-249 0.5252389659437391 1102 250-254 0.7017410163174418 1102 255-259 0.35753125235867067 1102 260-264 0.39318131234026943 1102 265-269 0.3326606444740836 1102 270-274 0.3044338653076686 1102 275-279 0.38637939223245077 1102 280-284 0.25625951474988184 1102 285-289 0.2713854664658282 1102 290-294 0.0059548915333049734 1102 295-299 -0.08207347277471655 1102 300-301 0.06758440161293855 1103 1 -8.025796327260082E-4 1103 2 0.05455322619054215 1103 3 0.08095345466926318 1103 4 0.07574143937529243 1103 5 -0.06314092300932117 1103 6 -0.061302574816160416 1103 7 0.8329156840563883 1103 8 0.2608706638717777 1103 9 0.07128024591146698 1103 10-14 0.07073569843418426 1103 15-19 0.16836905668751712 1103 20-24 0.16748121672988248 1103 25-29 -0.021517534306553898 1103 30-34 0.49029426916338537 1103 35-39 0.14615538118043503 1103 40-44 0.48169640361326316 1103 45-49 0.06626724690989505 1103 50-54 0.08160176503741212 1103 55-59 0.39655999112586926 1103 60-64 0.16647340830847668 1103 65-69 0.31307377744803944 1103 70-74 0.10860371839885374 1103 75-79 0.18319651495029632 1103 80-84 0.869545114893775 1103 85-89 0.5388866719297738 1103 90-94 0.49106938626361085 1103 95-99 1.2076127166431903 1103 100-104 0.5646494455544158 1103 105-109 0.7945036184719427 1103 110-114 0.5677837120071416 1103 115-119 0.8072086455909826 1103 120-124 1.0294683559771585 1103 125-129 0.8735920551820087 1103 130-134 1.0860790253334471 1103 135-139 0.9616856460804826 1103 140-144 1.5830135692168632 1103 145-149 1.5569612304140144 1103 150-154 2.2505766681063086 1103 155-159 1.2369534855449693 1103 160-164 1.1288331665457925 1103 165-169 1.8186247607457418 1103 170-174 2.255051026421736 1103 175-179 1.8715520866767257 1103 180-184 1.3777794970796258 1103 185-189 1.5816865489915521 1103 190-194 1.2104974851013104 1103 195-199 1.9819361443029635 1103 200-204 1.9979346862537746 1103 205-209 1.2495523868988592 1103 210-214 1.9215200780979131 1103 215-219 1.8200953210899407 1103 220-224 1.4521294165482992 1103 225-229 0.6864010308684527 1103 230-234 1.4112061644343186 1103 235-239 0.7716579286169925 1103 240-244 0.7242788681082928 1103 245-249 1.1854047853166243 1103 250-254 0.9419925886444851 1103 255-259 0.5259812165335003 1103 260-264 0.7244591526699367 1103 265-269 0.6586757858223997 1103 270-274 0.49553519557785464 1103 275-279 0.35739853311067904 1103 280-284 0.29616648303038495 1103 285-289 0.30921874482931777 1103 290-294 0.2754856765405638 1103 295-299 0.07925846546192616 1103 300-301 -0.06833611578596255 2116 1 -0.08462790499783779 2116 2 -0.07004661627566122 2116 3 0.09035851164880171 2116 4 -0.031713956157396694 2116 5 -0.08265560328074173 2116 6 -0.10637313984221208 2116 7 0.8049173027523295 2116 8 0.02937546576218608 2116 9 0.10548242392466278 2116 10-14 0.125623943377569 2116 15-19 0.12344446334874704 2116 20-24 0.14255835770818948 2116 25-29 0.1056808932459603 2116 30-34 0.42465831677038324 2116 35-39 0.0456748308238204 2116 40-44 0.32153366686091545 2116 45-49 0.10590939948644262 2116 50-54 0.10851836606499177 2116 55-59 0.3231499624373768 2116 60-64 0.2897501113717098 2116 65-69 0.40101232481296023 2116 70-74 0.43021330917228084 2116 75-79 0.34559796599558723 2116 80-84 0.8032898667780373 2116 85-89 0.4505709106007103 2116 90-94 0.4880278276906651 2116 95-99 1.174547238947234 2116 100-104 0.46194969197195945 2116 105-109 0.4562142332150856 2116 110-114 0.6373869147126783 2116 115-119 0.717160735081741 2116 120-124 0.6628814197205344 2116 125-129 0.8347953821802605 2116 130-134 0.7080708884958327 2116 135-139 1.0973677746656278 2116 140-144 1.663008221739208 2116 145-149 1.6831046150783102 2116 150-154 1.974063657115078 2116 155-159 1.7575383839790284 2116 160-164 1.4151450690193599 2116 165-169 1.4240473921383234 2116 170-174 1.5079592593694215 2116 175-179 1.8443261991038327 2116 180-184 1.7664442897203472 2116 185-189 1.1215151406538268 2116 190-194 1.0782341290532393 2116 195-199 0.6643732933747444 2116 200-204 0.6806233113016482 2116 205-209 0.5717147912404279 2116 210-214 1.7120940647870349 2116 215-219 1.566532383445864 2116 220-224 0.9284082947342007 2116 225-229 0.49946405161522023 2116 230-234 1.2393750869175726 2116 235-239 0.682887631687457 2116 240-244 0.6461818475205057 2116 245-249 0.7204437713192409 2116 250-254 0.6289974953165487 2116 255-259 1.5590380226440796 2116 260-264 0.6896738105397624 2116 265-269 0.5587357642879631 2116 270-274 0.8163209857399423 2116 275-279 0.5865422863173588 2116 280-284 0.31378714452782397 2116 285-289 0.11106073409100325 2116 290-294 0.08855086518367905 2116 295-299 0.1606745353549055 2116 300-301 -0.24085008065158853 1117 1 0.04295544782797123 1117 2 0.13446784114377408 1117 3 0.1259361786693347 1117 4 -0.00562942478650541 1117 5 0.06683429361905269 1117 6 0.1578692625904452 1117 7 0.663902134607234 1117 8 0.29641218958678905 1117 9 0.28018778765426333 1117 10-14 0.03370210520710515 1117 15-19 0.08751629162303232 1117 20-24 0.1296364655585549 1117 25-29 0.1328683988687942 1117 30-34 0.46120257068837134 1117 35-39 0.19646562557704073 1117 40-44 0.5195339187636776 1117 45-49 0.26650856820731406 1117 50-54 0.2671854405742309 1117 55-59 0.36162990917793536 1117 60-64 0.45623624772308347 1117 65-69 0.39374708919390855 1117 70-74 0.3659064916038659 1117 75-79 0.49657049060108704 1117 80-84 0.938042012449813 1117 85-89 0.6791381236564789 1117 90-94 0.6100603231474224 1117 95-99 1.1220420659440222 1117 100-104 0.5447695635015535 1117 105-109 0.6721909861884328 1117 110-114 0.9434790031665301 1117 115-119 0.8797834027320306 1117 120-124 1.097514811231413 1117 125-129 1.0349252920350622 1117 130-134 1.0284339883392946 1117 135-139 1.168885848689058 1117 140-144 1.421143781413182 1117 145-149 1.8235749535276717 1117 150-154 2.386067903475979 1117 155-159 1.9881175804092024 1117 160-164 1.9925130449806367 1117 165-169 1.5154488711674148 1117 170-174 1.4451210349240569 1117 175-179 1.6665415039961466 1117 180-184 1.7628237272574552 1117 185-189 1.4954907060855689 1117 190-194 -0.7766184178636557 1117 195-199 1.4357261911561991 1117 200-204 1.7692290294944293 1117 205-209 1.6900220046560541 1117 210-214 2.301555172799336 1117 215-219 1.9840302692619431 1117 220-224 1.9274690572798612 1117 225-229 1.73466782295948 1117 230-234 1.423569267963689 1117 235-239 1.273782786157831 1117 240-244 0.9168355351970625 1117 245-249 1.3078266815159054 1117 250-254 0.8575351997626317 1117 255-259 0.9310084420621809 1117 260-264 0.7282008990835838 1117 265-269 0.38549935354251197 1117 270-274 0.37329311449824054 1117 275-279 0.23391655984777593 1117 280-284 0.34335944961805964 1117 285-289 0.3675819568990004 1117 290-294 0.2210024188839501 1117 295-299 0.24053670855604992 1117 300-301 -0.15930465718504827 2117 1 0.19191979233492873 2117 2 0.17180390004498491 2117 3 0.062085530140961964 2117 4 0.0622643504265028 2117 5 0.11475665111466782 2117 6 0.13950605052076526 2117 7 0.8760423686925094 2117 8 0.13688144554293302 2117 9 0.2572640734047269 2117 10-14 0.10281117013229846 2117 15-19 0.2112261995446616 2117 20-24 0.22893674233635863 2117 25-29 0.2423638673106936 2117 30-34 0.47277405352996027 2117 35-39 0.18119581244728522 2117 40-44 0.6062247055000469 2117 45-49 0.19456913370234474 2117 50-54 0.24224866530695266 2117 55-59 0.5461556784574029 2117 60-64 0.3131041360712814 2117 65-69 0.43787162111380695 2117 70-74 0.3420268989918398 2117 75-79 0.409611609289918 2117 80-84 0.8730491580204571 2117 85-89 0.39333411128287565 2117 90-94 0.25980253990242375 2117 95-99 1.0076127166431945 2117 100-104 0.43553893342502903 2117 105-109 0.3839914890918976 2117 110-114 0.7214225260233107 2117 115-119 0.745483578205544 2117 120-124 0.9723254988342944 2117 125-129 0.8328912465566631 2117 130-134 0.7577771385409946 2117 135-139 1.3118204169699652 2117 140-144 2.056868016656214 2117 145-149 1.8992792897132027 2117 150-154 2.085617099373156 2117 155-159 2.112964267216121 2117 160-164 1.8091566166805642 2117 165-169 1.6302150572416956 2117 170-174 1.4388785196831861 2117 175-179 2.062926749749508 2117 180-184 1.5405827315809795 2117 185-189 1.2097188940050323 2117 190-194 1.6571282128641123 2117 195-199 2.154442882847441 2117 200-204 1.9340532846365228 2117 205-209 1.6279890445807972 2117 210-214 2.1641076791760803 2117 215-219 1.7599875043783477 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2110 145-149 -0.587323880154937 2110 150-154 -0.9746334785968607 2110 155-159 -1.0597995836445229 2110 160-164 -1.0773567059279188 2110 165-169 -1.764652640005 2110 170-174 -1.8290786803494825 2110 175-179 -0.8199099377631462 2110 180-184 -1.4100179665997032 2110 185-189 -1.6850110137683565 2110 190-194 -1.3085288262957953 2110 195-199 -1.558489440503262 2110 200-204 -1.418383777890778 2110 205-209 -0.9452883206571912 2110 210-214 -1.4780651329721195 2110 215-219 -1.11524235190155 2110 220-224 -1.4356279177225737 2110 225-229 -1.161074190109641 2110 230-234 -1.1036033629264779 2110 235-239 -1.3324214210735121 2110 240-244 -1.0203279879833875 2110 245-249 -0.7825911236144751 2110 250-254 -0.7525409336166824 2110 255-259 -0.7460127178886555 2110 260-264 -0.5105038608000516 2110 265-269 -0.6315216295595949 2110 270-274 -0.447282528125843 2110 275-279 -0.4580161315246585 2110 280-284 -0.30703996324414895 2110 285-289 -0.3550895200531894 2110 290-294 -0.45267877590608485 2110 295-299 -0.17801472347655967 2110 300-301 -0.40062271078789813 2109 1 -0.08724589445390762 2109 2 -0.027948266238773556 2109 3 -0.10225174079375421 2109 4 -0.037844189887174196 2109 5 -0.0043066459782608035 2109 6 -0.15756697902029515 2109 7 0.787392067493137 2109 8 0.1299692365666516 2109 9 -0.04867127274842176 2109 10-14 -0.06857977398752979 2109 15-19 -0.19973203052462196 2109 20-24 -0.15895413846829598 2109 25-29 -0.21451415146677988 2109 30-34 -4.153562529982771 2109 35-39 -0.12568202542966134 2109 40-44 0.021815797958311123 2109 45-49 -0.3756390827993954 2109 50-54 -0.25012999566737903 2109 55-59 -0.1253499565938725 2109 60-64 -0.13195167174000488 2109 65-69 -0.34236029335642115 2109 70-74 -0.25771766946996166 2109 75-79 -0.5544362208083129 2109 80-84 -2.1302110724263912E-4 2109 85-89 -0.5648887219811058 2109 90-94 -0.23960410638277807 2109 95-99 -0.03473645754591814 2109 100-104 -0.7564384901897299 2109 105-109 -0.33666807421424494 2109 110-114 -0.5128968357596406 2109 115-119 -0.6262568657243435 2109 120-124 -0.14033753776187297 2109 125-129 -0.8737995401797107 2109 130-134 -0.6824384948821773 2109 135-139 -1.3076242184250297 2109 140-144 -0.3318763528150086 2109 145-149 -0.6789753820427968 2109 150-154 -0.6313817609535484 2109 155-159 -1.4752381242887829 2109 160-164 -1.674869505354934 2109 165-169 -1.1210611959466803 2109 170-174 -1.3293756093712403 2109 175-179 -1.5941296369434532 2109 180-184 -1.4433507693868357 2109 185-189 -1.1925629851895998 2109 190-194 -1.357826471554926 2109 195-199 -1.9476295135417878 2109 200-204 -1.9365427825861126 2109 205-209 -1.3787028275587083 2109 210-214 -1.6332099278717997 2109 215-219 -0.7577146971810116 2109 220-224 -1.0639403386932962 2109 225-229 -0.9641241233492437 2109 230-234 -1.3189810676067815 2109 235-239 -1.1246064755871341 2109 240-244 -1.0807483574203864 2109 245-249 -0.7702941238532226 2109 250-254 -1.0549480217138765 2109 255-259 -0.7841593431727176 2109 260-264 -0.8696514499097052 2109 265-269 -0.4950375592091678 2109 270-274 -0.33167332845478015 2109 275-279 -0.21413713142739255 2109 280-284 -0.5173116913215363 2109 285-289 -0.9298677075305299 2109 290-294 -0.3502727489012827 2109 295-299 -0.27060423104126485 2109 300-301 -0.3787677444333699 2108 1 0.1600485407063701 2108 2 0.04870657428705982 2108 3 0.019084464176799543 2108 4 -0.042585965953094274 2108 5 0.06801035417366563 2108 6 -0.03753552208144839 2108 7 0.9770631101298051 2108 8 0.2934076944617203 2108 9 -0.020719280326687795 2108 10-14 -0.14254004172897083 2108 15-19 0.0020495761027916615 2108 20-24 -0.10626448083060325 2108 25-29 -0.08110134170082972 2108 30-34 0.2769521305593301 2108 35-39 -0.07802118842486294 2108 40-44 0.08617955456024617 2108 45-49 -0.14606384084012802 2108 50-54 -0.019890225874966916 2108 55-59 0.056122263890664215 2108 60-64 0.04053458819082323 2108 65-69 -0.003431080405462694 2108 70-74 -0.09502917359421303 2108 75-79 -0.4325280714950921 2108 80-84 -0.07520779317847825 2108 85-89 -0.32947285588757325 2108 90-94 -0.11885771901560105 2108 95-99 -0.35783389362666895 2108 100-104 -0.8146202433778669 2108 105-109 -0.5346158664197205 2108 110-114 -0.9002868928647061 2108 115-119 -0.5099623501343942 2108 120-124 -0.5270112345777562 2108 125-129 -0.378898245261972 2108 130-134 -0.48201239634690296 2108 135-139 -0.4581646164625468 2108 140-144 -0.07954165813498548 2108 145-149 -0.5699606455393393 2108 150-154 -0.6300852346217667 2108 155-159 -1.815707297382378 2108 160-164 -1.206020990176068 2108 165-169 -1.2953540850699028 2108 170-174 -1.9002000135214168 2108 175-179 -1.276515359782799 2108 180-184 -1.8864364091155252 2108 185-189 -1.2719056066607521 2108 190-194 -1.288575808010119 2108 195-199 -1.374094808137805 2108 200-204 -1.176974958030037 2108 205-209 -1.296852945075397 2108 210-214 -1.0502796067786733 2108 215-219 -0.9985536680386637 2108 220-224 -0.7151354342705574 2108 225-229 -1.2844694347461285 2108 230-234 -1.125635997498918 2108 235-239 -0.65357635020621 2108 240-244 -0.746341436893843 2108 245-249 -1.109917534250096 2108 250-254 -0.5698080293993826 2108 255-259 -0.6281654558278813 2108 260-264 -0.697694238495842 2108 265-269 -0.5891928242249396 2108 270-274 -0.5686606116297863 2108 275-279 -0.2785298427116736 2108 280-284 -0.7585921317186806 2108 285-289 -1.1261450647902027 2108 290-294 -0.794248119548028 2108 295-299 -0.7369108110821969 2108 300-301 2.4618706884391326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 25.0 11 252.0 12 480.0 13 572.0 14 742.0 15 1020.0 16 1400.0 17 2008.0 18 2745.0 19 3465.0 20 4547.0 21 6013.0 22 7860.0 23 10736.0 24 15148.0 25 21327.0 26 29248.0 27 36909.0 28 43054.0 29 42211.0 30 31214.0 31 14779.0 32 3735.0 33 411.0 34 21.0 35 3.0 36 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.299203006498125 19.541166089730535 12.139593536886402 33.020037366884935 2 51.972478539047685 20.508561160588297 14.759562314460556 12.759397985903467 3 50.801816187791815 19.686561139154136 16.163857005576453 13.347765667477592 4 51.161552833417275 18.840626306144102 16.24780746408885 13.750013396349765 5 33.834535432452036 19.05568237433331 33.80631378895212 13.303468404262539 6 33.02539590678998 18.673082625112976 33.907054339167 14.39446712893004 7 15.585134695831412 52.70374061808971 17.401679723642236 14.309444962436634 8 32.24662144058987 17.68175274267934 35.04949504692295 15.022130769807843 9 14.263004283259564 16.68863668027736 36.06368803295145 32.98467100351163 10-14 27.701079209936875 36.53738296055757 20.95910719580462 14.802430633700931 15-19 22.50879693634412 35.437024652855925 19.395485251512 22.658693159287957 20-24 24.914781353710076 30.00689465467783 26.033430144287617 19.04489384732448 25-29 19.311606240198337 24.562189427958003 38.633643771411826 17.492560560431826 30-34 21.402079827955145 26.904871627245676 29.685167918778827 22.007880626020356 35-39 20.706398453882617 29.230763734830866 27.65013735723957 22.41270045404695 40-44 22.513441004261825 26.995966805631465 29.2114729911727 21.27911919893401 45-49 22.13462795657439 29.13395278054636 25.53665777149042 23.194761491388828 50-54 22.688272299563813 28.078391866450897 27.43693891621744 21.796396917767847 55-59 22.11740918167951 26.650876835746462 27.48037881304769 23.751335169526342 60-64 22.84424153440004 25.61889349723321 27.89327217453122 23.643592793835534 65-69 19.22801301767961 28.862810661350995 27.705151700264715 24.204024620704683 70-74 21.53975857991548 25.676122703419107 28.629964240677037 24.15415447598838 75-79 20.687536393416856 24.68893675851204 27.911991340599513 26.71153550747159 80-84 26.786555066142242 23.10223736902835 26.849928731419265 23.26127883341014 85-89 21.262043318436593 28.327456801237467 24.952719816237806 25.457780064088137 90-94 21.594987264536826 29.73239451714197 27.642206718180095 21.030411500141106 95-99 21.75702951126544 29.65716061687511 25.89996677705259 22.68584309480686 100-104 22.79187073772805 28.571377537715193 24.4090066338724 24.22774509068436 105-109 25.481214745272872 25.17477770990294 23.33808457204914 26.005922972775046 110-114 20.427968720416395 27.657567865907893 29.513051617028722 22.401411796646983 115-119 25.144698439235945 26.613581398007334 24.600199337684465 23.641520825072252 120-124 18.689586928020518 26.83006641017122 28.272585352609784 26.207761309198467 125-129 21.7563150392781 23.360018862060468 30.88348033594473 24.000185762716708 130-134 26.243842144559114 27.689576210940707 24.306051220496773 21.760530424003402 135-139 25.229077580940746 26.92916367481522 23.527848331886528 24.313910412357504 140-144 21.633640199051897 26.757976186648662 26.243627802962916 25.364755811336526 145-149 19.90590403926738 26.260775130659063 28.82337180764985 25.009949022423704 150-154 22.879036320183477 24.140079377837793 26.46711464060273 26.513769661376003 155-159 23.487695006198045 24.82332893933061 29.15174313303111 22.537232921440236 160-164 26.001564693652274 27.240101883705393 24.640781346565355 22.117552076076976 165-169 21.965583884369856 24.330128926470117 27.80703540565933 25.897251783500696 170-174 24.051199062612753 28.135621072636795 25.494932607429792 22.31824725732066 175-179 22.26537633025753 28.603314435549272 26.206546706819996 22.924762527373208 180-184 21.189667306119095 28.300521207314766 26.551636676704998 23.958174809861145 185-189 22.710992508761212 26.074297941963444 27.657925101901565 23.55678444737378 190-194 21.241466525201215 26.218835625002235 25.881247610984293 26.658450238812257 195-199 21.510036545242155 28.208425768146693 28.39090191371322 21.890635772897934 200-204 23.316650412428956 27.405287807178304 25.07067914134757 24.20738263904518 205-209 22.812376083764697 26.453968356035677 27.4549436102984 23.278711949901222 210-214 24.967223597580798 26.677526640874227 26.01285335105224 22.342396410492736 215-219 24.59012528266298 25.916685421556334 27.786315718026483 21.7068735777542 220-224 20.683535350287755 27.06076941488317 27.87369564207811 24.381999592750965 225-229 23.93016750795743 27.398000192907435 25.428200923812277 23.243631375322853 230-234 22.950197730122497 26.609794696474438 27.465017665319884 22.97498990808318 235-239 23.03114740628807 26.574714121896065 24.729590214591664 25.664548257224208 240-244 24.366209761830763 29.549418241184313 23.43346658235897 22.650905414625953 245-249 23.372236333043972 28.76842891182344 22.86931950115566 24.99001525397693 250-254 23.350587831827582 27.00082521514538 23.83564286403241 25.812944088994634 255-259 23.73583112740107 27.522032529909584 25.795510972503543 22.9466253701858 260-264 23.930953427143507 28.975554340953174 24.284617060876585 22.80887517102673 265-269 22.846456397560793 28.858523829426957 25.39519231799719 22.899827455015057 270-274 22.24872913295252 30.213805742211363 23.638305701129223 23.899159423706895 275-279 22.864889774834154 30.187798961872204 22.254373461652502 24.692937801641143 280-284 20.79184930356843 30.645704058558128 24.9445033883834 23.617943249490043 285-289 22.436706712821557 30.02647118713093 24.890989436531665 22.645832663515844 290-294 21.455665227005614 32.09086654734985 23.590078841983804 22.86338938366074 295-299 20.753696499444498 33.24323841572981 21.691798218821333 24.311266866004352 300-301 16.32747109067721 35.46924733948494 27.89030711578376 20.312974454054093 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.25 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.25 26 0.25 27 0.5 28 0.75 29 1.0 30 1.0 31 2.0 32 2.5 33 9.75 34 26.25 35 91.25 36 245.5 37 697.25 38 1493.75 39 2572.25 40 3941.25 41 5938.75 42 8352.5 43 10071.0 44 10473.25 45 9031.75 46 6831.75 47 5325.5 48 4458.75 49 4082.0 50 3621.0 51 2840.25 52 1712.0 53 914.5 54 579.25 55 551.75 56 612.25 57 696.25 58 815.75 59 1030.5 60 1267.25 61 1355.0 62 1276.0 63 975.75 64 619.75 65 349.0 66 197.25 67 112.75 68 56.0 69 33.75 70 28.75 71 16.75 72 11.25 73 8.75 74 3.5 75 3.0 76 3.5 77 2.5 78 1.75 79 2.0 80 0.75 81 1.0 82 0.5 83 0.5 84 0.25 85 0.5 86 0.5 87 0.25 88 0.25 89 1.25 90 0.5 91 0.25 92 0.25 93 0.25 94 1.0 95 0.25 96 0.5 97 0.5 98 0.75 99 0.75 100 1.6666666666666665 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260-264 0.0 265-269 0.0 270-274 0.0 275-279 0.0 280-284 0.0 285-289 0.0 290-294 0.0 295-299 0.0 300-301 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 301 279927.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.349902329153107 #Duplication Level Percentage of deduplicated Percentage of total 1 49.85559825664142 8.151341620575831 2 9.457509072555832 3.092586992267345 3 7.776345693589398 3.8142747770375114 4 6.5936883653688465 4.312246430506154 5 4.748554206679153 3.881919874194664 6 3.8928564399407337 3.818869354466741 7 2.959546208823769 3.3871804017018796 8 2.4254034727130964 3.172408791011831 9 1.933542467953842 2.845190745028347 >10 9.945814405723805 29.10181057804357 >50 0.28720061737509806 3.065594793134088 >100 0.07212654046332019 2.3712501279195846 >500 0.013516761436084537 1.5333559116991193 >1k 0.024780729299488217 10.994268702451807 >5k 0.011263967863403735 11.966658711312164 >10k+ 0.002252793572680747 4.491042188649365 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC 12193 4.355778470815606 No Hit GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 7831 2.797515066428033 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT 7358 2.628542441422228 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA 6008 2.1462738499680274 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG 5761 2.058036559531592 No Hit GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG 5531 1.9758722809875433 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA 3924 1.4017940391602095 No Hit GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC 3656 1.306054792856709 No Hit GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC 3637 1.2992673089769833 No Hit GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA 3223 1.151371607597695 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT 3126 1.1167197162117266 No Hit GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 2847 1.0170508739778585 No Hit GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA 2659 0.9498905071679402 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 2003 0.7155436953205657 No Hit GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 1790 0.639452428668903 No Hit GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 1598 0.5708631178843055 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 1386 0.4951290872263126 No Hit GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 961 0.34330378991665683 No Hit GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA 731 0.2611395113726079 No Hit GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 685 0.24470665566379804 No Hit GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG 626 0.22362973203728115 No Hit GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA 624 0.2229152600499416 No Hit CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT 536 0.19147849260700112 No Hit GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 470 0.16790091702479573 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 456 0.16289961311341886 Illumina Single End PCR Primer 1 (96% over 32bp) GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 359 0.12824772172745036 No Hit GGGGGGAGCAAAACCAGAAGAAGTGTCATTCCGGGGGAGGGGAGTTTTCG 347 0.12396088980341302 No Hit CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT 321 0.1146727539679988 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 289 0.10324120217056588 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-14 5.715775898716451E-4 0.0 0.0 15-19 0.0010002607822753788 0.0 0.0 20-24 0.0017147327696149354 0.0 0.0 25-29 0.0017861799683488908 0.0 0.0 30-34 0.002572099154422403 0.0 0.0 35-39 0.003072229545560092 0.0 0.0 40-44 0.004001043129101515 0.0 0.0 45-49 0.007430508668331387 0.0 0.0 50-54 0.016647197305011664 0.0 0.0 55-59 0.022648761998663936 0.0 0.0 60-64 0.02650691073029754 0.0 0.0 65-69 0.035223468975840125 0.0 0.0 70-74 0.04701225676694281 0.0 0.0 75-79 0.06065867172512833 0.0 0.0 80-84 0.08187848974911317 0.0 0.0 85-89 0.10881408367181444 0.0 0.0 90-94 0.14725267659068259 0.0 0.0 95-99 0.19798018769179107 0.0 0.0 100-104 0.2633543745333605 0.0 0.0 105-109 0.3443754978976662 0.0 0.0 110-114 0.4418294769707817 0.0 0.0 115-119 0.557359597323588 0.0 0.0 120-124 0.7012542555737745 0.0 0.0 125-129 0.8774430476517093 0.0 0.0 130-134 1.0812104584409508 0.0 0.0 135-139 1.3137710903199762 0.0 0.0 140-144 1.560906950740729 0.0 0.0 145-149 1.8599134774423334 0.0 0.0 150-154 2.2243656381842407 0.0 0.0 155-159 2.640759912405734 0.0 0.0 160-164 3.104166443394171 0.0 0.0 165-169 3.5918650219521515 0.0 0.0 170-174 4.140579508228932 0.0 0.0 175-179 4.7592408020662536 0.0 0.0 180-184 5.462066896012175 0.0 0.0 185-189 6.357371743347373 0.0 0.0 190-194 7.224955077573796 0.0 0.0 195-199 8.037881304768742 0.0 0.0 200-204 8.828301664362495 0.0 0.0 205-209 9.650230238597919 0.0 0.0 210-214 10.493592972453532 0.0 0.0 215-219 11.303732758897855 0.0 0.0 220-224 12.111514787783957 0.0 0.0 225-229 12.89493332190178 0.0 0.0 230-234 13.63548353677923 0.0 0.0 235-239 14.27900845577597 0.0 0.0 240-244 14.844584480953964 0.0 0.0 245-249 15.303204049627226 0.0 0.0 250-254 15.709881504820899 0.0 0.0 255-259 16.06411671614385 0.0 0.0 260-264 16.351977479842958 0.0 0.0 265-269 16.56581894565369 0.0 0.0 270-274 16.725074751631677 0.0 0.0 275-279 16.83260278572628 0.0 0.0 280-284 16.914338381077926 0.0 0.0 285-289 16.968852593711933 2.1434159620186693E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTTAT 4650 0.0 289.2903 2 CGGGTTA 4695 0.0 286.2034 1 GGTTATT 4690 0.0 285.25052 3 GTTATTA 4685 0.0 284.61047 4 TTATTAG 4700 0.0 283.3883 5 GGGGGGA 5185 0.0 282.19864 1 TTAGTAG 4805 0.0 282.10718 8 TATTAGT 4880 0.0 273.84222 6 GGGGAGC 5360 0.0 273.81064 3 GGAGCGA 4050 0.0 272.05557 5 GGGAGCG 4105 0.0 270.20706 4 CGAAAGC 4115 0.0 267.7582 9 ATTAGTA 5020 0.0 265.91135 7 TAGTAGA 5160 0.0 262.41278 9 GCGAAAG 4220 0.0 261.44553 8 GAGCGAA 4240 0.0 259.5165 6 AGCGAAA 4260 0.0 257.60565 7 GGGGGAG 5860 0.0 252.2099 2 GGGAGCA 1500 0.0 236.0 4 CAAAAGC 1790 0.0 185.40501 9 >>END_MODULE